Open reading frames E6 and E7 of bovine papillomavirus type 1 are both required for full transformation of mouse C127 cells.

1989 ◽  
Vol 63 (1) ◽  
pp. 259-266 ◽  
Author(s):  
K Neary ◽  
D DiMaio
Keyword(s):  
Open Reading Frames ◽  
Bovine Papillomavirus ◽  
Full Transformation ◽  
E6 And E7 ◽  
Reading Frames

scholarly journals The First Complete Papillomavirus Genome Characterized from a Marsupial Host: a Novel Isolate from Bettongia penicillata

2010 ◽  
Vol 84 (10) ◽  
pp. 5448-5453 ◽  
Author(s):  
Mark D. Bennett ◽  
Andrea Reiss ◽  
Hans Stevens ◽  
Elisabeth Heylen ◽  
Marc Van Ranst ◽  
...  
Keyword(s):  
Open Reading Frames ◽  
Double Stranded Dna ◽  
Bettongia Penicillata ◽  
European Hedgehog ◽  
Reading Frames

ABSTRACT The first fully sequenced papillomavirus (PV) of marsupials, tentatively named Bettongia penicillata papillomavirus type 1 (BpPV1), was detected in papillomas from a woylie (Bettongia penicillata ogilbyi). The circular, double-stranded DNA genome contains 7,737 bp and encodes 7 open reading frames (ORFs), E6, E7, E1, E2, E4, L2, and L1, in typical PV conformation. BpPV1 is a close-to-root PV with L1 and L2 ORFs most similar to European hedgehog PV and bandicoot papillomatosis carcinomatosis virus types 1 and 2 (BPCV1 and -2). It appears that the BPCVs arose by recombination between an ancient PV and an ancient polyomavirus more than 10 million years ago.

scholarly journals Opposing effects of bovine papillomavirus type 1 E6 and E7 genes on Fas-mediated apoptosis

Oncogene ◽  
2005 ◽  
Vol 24 (24) ◽  
pp. 3942-3953 ◽  
Author(s):  
Yun Liu ◽  
Zhiguo Liu ◽  
Hua Gao ◽  
You Zhou ◽  
Elliot J Androphy ◽  
...  
Keyword(s):  
Bovine Papillomavirus ◽  
E6 And E7 ◽  
Opposing Effects

scholarly journals Mutagenic Potential ofBos taurusPapillomavirus Type 1 E6 Recombinant Protein: First Description

2015 ◽  
Vol 2015 ◽  
pp. 1-15 ◽  
Author(s):  
Rodrigo Pinheiro Araldi ◽  
Jacqueline Mazzuchelli-de-Souza ◽  
Diego Grando Modolo ◽  
Edislane Barreiros de Souza ◽  
Thatiana Corrêa de Melo ◽  
...  
Keyword(s):  
Bos Taurus ◽  
Micronucleus Assay ◽  
Positive Control ◽  
Bovine Papillomavirus ◽  
Mutagenic Potential ◽  
E6 Oncoprotein ◽  
Per Se ◽  
E6 And E7 ◽  
Negative Controls

Bovine papillomavirus (BPV) is considered a useful model to study HPV oncogenic process. BPV interacts with the host chromatin, resulting in DNA damage, which is attributed to E5, E6, and E7 viral oncoproteins activity. However, the oncogenic mechanisms of BPV E6 oncoproteinper seremain unknown. This study aimed to evaluate the mutagenic potential ofBos tauruspapillomavirus type 1 (BPV-1) E6 recombinant oncoprotein by the cytokinesis-block micronucleus assay (CBMNA) and comet assay (CA). Peripheral blood samples of five calves were collected. Samples were subjected to molecular diagnosis, which did not reveal presence of BPV sequences. Samples were treated with 1 μg/mL of BPV-1 E6 oncoprotein and 50 μg/mL of cyclophosphamide (positive control). Negative controls were not submitted to any treatment. The samples were submitted to the CBMNA and CA. The results showed that BPV E6 oncoprotein induces clastogenesisper se, which is indicative of genomic instability. These results allowed better understanding the mechanism of cancer promotion associated with the BPV E6 oncoprotein and revealed that this oncoprotein can induce carcinogenesisper se. E6 recombinant oncoprotein has been suggested as a possible vaccine candidate. Results pointed out that BPV E6 recombinant oncoprotein modifications are required to use it as vaccine.

scholarly journals Control of the Papillomavirus Early-to-Late Switch by Differentially Expressed SRp20

2008 ◽  
Vol 83 (1) ◽  
pp. 167-180 ◽  
Author(s):  
Rong Jia ◽  
Xuefeng Liu ◽  
Mingfang Tao ◽  
Michael Kruhlak ◽  
Ming Guo ◽  
...  
Keyword(s):  
Keratinocyte Differentiation ◽  
Bovine Papillomavirus ◽  
Cellular Transcription Factor ◽  
Monolayer Cultures ◽  
Papillomavirus Infection ◽  
Alternative Mrna Splicing ◽  
E6 And E7 ◽  
Cellular Transcription ◽  
Selection Of

ABSTRACT The viral early-to-late switch of papillomavirus infection is tightly linked to keratinocyte differentiation and is mediated in part by alternative mRNA splicing. Here, we report that SRp20, a cellular splicing factor, controls the early-to-late switch via interactions with A/C-rich RNA elements. An A/C-rich SE4 element regulates the selection of a bovine papillomavirus type 1 (BPV-1) late-specific splice site, and binding of SRp20 to SE4 suppresses this selection. Expression of late BPV-1 L1 or human papillomavirus (HPV) L1, the major capsid protein, inversely correlates with SRp20 levels in the terminally differentiated keratinocytes. In HPV type 16, a similar SRp20-interacting element also controls the viral early-to-late switch. Keratinocytes in raft cultures, which support L1 expression, make considerably less SRp20 than keratinocytes in monolayer cultures, which do not support L1 expression. Conversely, abundant SRp20 in cancer cells or undifferentiated keratinocytes is important for the expression of the viral early E6 and E7 by promoting the expression of cellular transcription factor SP1 for transactivation of viral early promoters.

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