scholarly journals Characterization of the Human Cytomegalovirus UL34 Gene

2004 ◽  
Vol 78 (17) ◽  
pp. 9579-9583 ◽  
Author(s):  
B. J. Biegalke ◽  
E. Lester ◽  
A. Branda ◽  
R. Rana
Keyword(s):  
Tissue Culture ◽  
Viral Replication ◽  
Human Cytomegalovirus ◽  
Immune Evasion ◽  
Transcriptional Repressor ◽  
Replication Cycle ◽  
Differentially Expressed ◽  
Minor Protein

ABSTRACT UL34 encodes the transcriptional repressor of the human cytomegalovirus immune evasion gene, US3, and is essential for viral replication in tissue culture. Two different monocistronic transcripts originate from UL34 at early and late times postinfection and encode two predominant proteins and a third, minor protein. The UL34 proteins are differentially expressed throughout the viral replication cycle, with both proteins localizing to the nucleus and repressing expression of the US3 gene.

Wedelolactone inhibits human cytomegalovirus replication by targeting distinct steps of the viral replication cycle

2020 ◽  
Vol 174 ◽  
pp. 104677 ◽  
Author(s):  
Adriana Svrlanska ◽  
Anna Ruhland ◽  
Manfred Marschall ◽  
Nina Reuter ◽  
Thomas Stamminger
Keyword(s):  
Viral Replication ◽  
Human Cytomegalovirus ◽  
Replication Cycle

scholarly journals Transcriptomic Characterization of Postmolar Gestational Choriocarcinoma

Biomedicines ◽  
2021 ◽  
Vol 9 (10) ◽  
pp. 1474
Author(s):  
Constance Collet ◽  
Jonathan Lopez ◽  
Christophe Battail ◽  
Fabienne Allias ◽  
Mojgan Devouassoux-Shisheboran ◽  
...  
Keyword(s):  
Immune Evasion ◽  
Differentially Expressed ◽  
Tissue Invasion ◽  
Gestational Choriocarcinoma ◽  
Upstream Regulator ◽  
Term Delivery ◽  
Canonical Pathways ◽  
Placental Diseases ◽  
Hydatidiform Moles

The human placenta shares properties with solid tumors, such as rapid growth, tissue invasion, cell migration, angiogenesis, and immune evasion. However, the mechanisms that drive the evolution from premalignant proliferative placental diseases—called hydatidiform moles—to their malignant counterparts, gestational choriocarcinoma, as well as the factors underlying the increased aggressiveness of choriocarcinoma arising after term delivery compared to those developing from hydatidiform moles, are unknown. Using a 730-gene panel covering 13 cancer-associated canonical pathways, we compared the transcriptomic profiles of complete moles to those of postmolar choriocarcinoma samples and those of postmolar to post-term delivery choriocarcinoma. We identified 33 genes differentially expressed between complete moles and postmolar choriocarcinoma, which revealed TGF-β pathway dysregulation. We found the strong expression of SALL4, an upstream regulator of TGF-β, in postmolar choriocarcinoma, compared to moles, in which its expression was almost null. Finally, there were no differentially expressed genes between postmolar and post-term delivery choriocarcinoma samples. To conclude, the TGF-β pathway appears to be a crucial step in the progression of placental malignancies. Further studies should investigate the value of TGF- β family members as biomarkers and new therapeutic targets.

scholarly journals Inhibition of Human Cytomegalovirus Replication by Benzimidazole Nucleosides Involves Three Distinct Mechanisms

2004 ◽  
Vol 48 (10) ◽  
pp. 3918-3927 ◽  
Author(s):  
David L. Evers ◽  
Gloria Komazin ◽  
Roger G. Ptak ◽  
Dongjin Shin ◽  
Brian T. Emmer ◽  
...  
Keyword(s):  
Viral Replication ◽  
Dna Synthesis ◽  
Human Cytomegalovirus ◽  
Cellular Response ◽  
Replication Cycle ◽  
Host Cells ◽  
Viral Dna ◽  
Marked Contrast ◽  
Nuclear Egress ◽  
Hcmv Replication

ABSTRACT The benzimidazole nucleosides 2-bromo-5,6-dichloro-1-(β-d-ribofuranosyl)benzimidazole (BDCRB) and 2-isopropylamino-5,6-dichloro-1-(β-l-ribofuranosyl)benzimidazole (1263W94, or maribavir) are potent and selective inhibitors of human cytomegalovirus (HCMV) replication. These inhibitors act by two different mechanisms: BDCRB blocks the processing and maturation of viral DNA, whereas maribavir prevents viral DNA synthesis and capsid nuclear egress. In order to determine by which of these two mechanisms other benzimidazole nucleosides acted, we performed time-of-addition studies and other experiments with selected new analogs. We found that the erythrofuranosyl analog and the α-lyxofuranosyl analog acted late in the viral replication cycle, similar to BDCRB. In marked contrast, the α-5′-deoxylyxofuranosyl analog of 2,5,6-trichloro-1-(β-d-ribofuranosyl)benzimidazole (compound UMJD1311) acted early in the replication cycle, too early to be consistent with either mechanism. Similar to other reports on early acting inhibitors of herpesviruses, compound 1311 was multiplicity of infection dependent, an observation that could not be reproduced with UV-inactivated virus. HCMV isolates resistant to BDCRB and maribavir were sensitive to compound 1311, as were viruses resistant to ganciclovir, cidofovir, and foscarnet. The preincubation of host cells with compound 1311 and removal prior to the addition of HCMV did not produce an antiviral cellular response. We conclude that this newly discovered early mode of action occurs at a stage of viral replication after entry to cells but prior to viral DNA synthesis, thereby strongly suggesting that the trisubstituted benzimidazole nucleoside series possesses three distinct biochemical modes of action for inhibition of HCMV replication.

scholarly journals Genome-Wide Identification and Characterization of Long Noncoding RNAs Involved in Chinese Wheat Mosaic Virus Infection of Nicotiana benthamiana

Biology ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 232
Author(s):  
Weiran Zheng ◽  
Haichao Hu ◽  
Qisen Lu ◽  
Peng Jin ◽  
Linna Cai ◽  
...  
Keyword(s):  
Virus Infection ◽  
Mosaic Virus ◽  
Nicotiana Benthamiana ◽  
Noncoding Rnas ◽  
Long Noncoding Rnas ◽  
Differentially Expressed ◽  
Genome Wide ◽  
Chinese Wheat

Recent studies have shown that a large number of long noncoding RNAs (lncRNAs) can regulate various biological processes in animals and plants. Although lncRNAs have been identified in many plants, they have not been reported in the model plant Nicotiana benthamiana. Particularly, the role of lncRNAs in plant virus infection remains unknown. In this study, we identified lncRNAs in N. benthamiana response to Chinese wheat mosaic virus (CWMV) infection by RNA sequencing. A total of 1175 lncRNAs, including 65 differentially expressed lncRNAs, were identified during CWMV infection. We then analyzed the functions of some of these differentially expressed lncRNAs. Interestingly, one differentially expressed lncRNA, XLOC_006393, was found to participate in CWMV infection as a precursor to microRNAs in N. benthamiana. These results suggest that lncRNAs play an important role in the regulatory network of N. benthamiana in response to CWMV infection.

scholarly journals Functional and structural characterization of AntR, an Sb(III) responsive transcriptional repressor

2021 ◽  
Author(s):  
Thiruselvam Viswanathan ◽  
Jian Chen ◽  
Minghan Wu ◽  
Lijin An ◽  
Palani Kandavelu ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Transcriptional Repressor

scholarly journals Biochemical and structural characterization of the capsid-bound tegument proteins of human cytomegalovirus

2011 ◽  
Vol 174 (3) ◽  
pp. 451-460 ◽  
Author(s):  
Xuekui Yu ◽  
Sanket Shah ◽  
Manfred Lee ◽  
Wei Dai ◽  
Pierrette Lo ◽  
...  
Keyword(s):  
Human Cytomegalovirus ◽  
Structural Characterization ◽  
Tegument Proteins

scholarly journals Transcriptome-(phospho)proteome characterization of brain of a germline model of cytoplasmic-predominant Pten expression with autism-like phenotypes

2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Stetson Thacker ◽  
Charis Eng
Keyword(s):  
Autism Spectrum ◽  
Pten Expression ◽  
Differentially Expressed ◽  
Risk Genes ◽  
Animal Modeling ◽  
Network Analyses ◽  
Phosphorylated Proteins ◽  
Mass Spectrometry Technology ◽  
Neurological Processes

AbstractPTEN has a strong Mendelian association with autism spectrum disorder (ASD), representing a special case in autism’s complex genetic architecture. Animal modeling for constitutional Pten mutation creates an opportunity to study how disruption of Pten affects neurobiology and glean potential insight into ASD pathogenesis. Subsequently, we comprehensively characterized the neural (phospho)proteome of Ptenm3m4/m3m4 mice, which exhibits cytoplasmic-predominant Pten expression, by applying mass spectrometry technology to their brains at two-weeks- (P14) and six-weeks-of-age (P40). The differentially expressed/phosphorylated proteins were subjected to gene enrichment, pathway, and network analyses to assess the affected biology. We identified numerous differentially expressed/phosphorylated proteins, finding greater dysregulation at P40 consistent with prior transcriptomic data. The affected pathways were largely related to PTEN function or neurological processes, while scant direct overlap was found across datasets. Network analysis pointed to ASD risk genes like Pten and Psd-95 as major regulatory hubs, suggesting they likely contribute to initiation or maintenance of cellular and perhaps organismal phenotypes related to ASD.

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