Molecular and developmental characterization of the heat shock cognate 4 gene of Drosophila melanogaster

The Drosophila heat shock cognate gene 4 (hsc4), a member of the hsp70 gene family, encodes an abundant protein, hsc70, that is more similar to the constitutively expressed human protein than the Drosophila heat-inducible hsp70. Developmental expression revealed that hsc4 transcripts are enriched in cells active in endocytosis and those undergoing rapid growth and changes in shape.

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Identification and sequence analysis of a new member of the mouse HSP70 gene family and characterization of its unique cellular and developmental pattern of expression in the male germ line.

Molecular and Cellular Biology ◽

10.1128/mcb.8.7.2925 ◽

1988 ◽

Vol 8 (7) ◽

pp. 2925-2932 ◽

Cited By ~ 119

Author(s):

Z F Zakeri ◽

D J Wolgemuth ◽

C R Hunt

Keyword(s):

Heat Shock ◽

Gene Family ◽

Developmental Stages ◽

Germ Line ◽

Cell Lineage ◽

Hsp70 Gene ◽

Coding Region ◽

Male Germ Line ◽

Sequence Organization

A unique member of the mouse HSP70 gene family has been isolated and characterized with respect to its DNA sequence organization and expression. The gene contains extensive similarity to a heat shock-inducible HSP70 gene within the coding region but diverges in both 3' and 5' nontranslated regions. The gene does not yield transcripts in response to heat shock in mouse L cells. Rather, the gene appears to be activated uniquely in the male germ line. Analysis of RNA from different developmental stages and from enriched populations of spermatogenic cells revealed that this gene is expressed during the prophase stage of meiosis. A transcript different in size from the major heat-inducible mouse transcripts is most abundant in meiotic prophase spermatocytes and decreases in abundance in postmeiotic stages of spermatogenesis. This pattern of expression is distinct from that observed for another member of this gene family, which was previously shown to be expressed abundantly in postmeiotic germ cells. These observations suggest that specific HSP70 gene family members play distinct roles in the differentiation of the germ cell lineage in mammals.

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Heat shock and developmental regulation of the Drosophila melanogaster hsp83 gene.

Molecular and Cellular Biology ◽

10.1128/mcb.9.4.1746 ◽

1989 ◽

Vol 9 (4) ◽

pp. 1746-1753 ◽

Cited By ~ 63

Author(s):

H Xiao ◽

J T Lis

Keyword(s):

Drosophila Melanogaster ◽

Heat Shock ◽

Fusion Gene ◽

Developmental Expression ◽

Regulatory Sequence ◽

Mrna Levels ◽

Hsp70 Gene ◽

High Level ◽

Hsp83 Gene

In contrast to the hsp70 gene, whose expression is normally at a very low level and increases by more than 2 orders of magnitude during heat shock, the hsp83 gene in Drosophila melanogaster is expressed at high levels during normal development and increases only severalfold in response to heat shock. Developmental expression of the hsp83 gene consists of a high level of tissue-general, basal expression and a very high level of expression in ovaries. We identified regions upstream of the hsp83 gene that were required for its developmental and heat shock-induced expression by assaying beta-galactosidase activity and mRNA levels in transgenic animals containing a series of 5' deletion and insertion mutations of an hsp83-lacZ fusion gene. Deletion of sequences upstream of the overlapping array of a previously defined heat shock consensus (HSC) sequence eliminated both forms of developmental expression of the hsp83 gene. As a result, the hsp83 gene with this deletion mutation was regulated like that of the hsp70 gene. Moreover, an in vivo polymer competition assay revealed that the overlapping HSC sequences of the hsp83 gene and the nonoverlapping HSC sequences of the hsp70 gene had similar affinities for the factor required for heat induction of the two heat shock genes. We discuss the functional similarity of hsp70 and hsp83 heat shock regulation in terms of a revised view of the heat shock-regulatory sequence.

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Heat shock and developmental regulation of the Drosophila melanogaster hsp83 gene

Molecular and Cellular Biology ◽

10.1128/mcb.9.4.1746-1753.1989 ◽

1989 ◽

Vol 9 (4) ◽

pp. 1746-1753

Author(s):

H Xiao ◽

J T Lis

Keyword(s):

Drosophila Melanogaster ◽

Heat Shock ◽

Fusion Gene ◽

Developmental Expression ◽

Regulatory Sequence ◽

Mrna Levels ◽

Hsp70 Gene ◽

High Level ◽

Hsp83 Gene

In contrast to the hsp70 gene, whose expression is normally at a very low level and increases by more than 2 orders of magnitude during heat shock, the hsp83 gene in Drosophila melanogaster is expressed at high levels during normal development and increases only severalfold in response to heat shock. Developmental expression of the hsp83 gene consists of a high level of tissue-general, basal expression and a very high level of expression in ovaries. We identified regions upstream of the hsp83 gene that were required for its developmental and heat shock-induced expression by assaying beta-galactosidase activity and mRNA levels in transgenic animals containing a series of 5' deletion and insertion mutations of an hsp83-lacZ fusion gene. Deletion of sequences upstream of the overlapping array of a previously defined heat shock consensus (HSC) sequence eliminated both forms of developmental expression of the hsp83 gene. As a result, the hsp83 gene with this deletion mutation was regulated like that of the hsp70 gene. Moreover, an in vivo polymer competition assay revealed that the overlapping HSC sequences of the hsp83 gene and the nonoverlapping HSC sequences of the hsp70 gene had similar affinities for the factor required for heat induction of the two heat shock genes. We discuss the functional similarity of hsp70 and hsp83 heat shock regulation in terms of a revised view of the heat shock-regulatory sequence.

Download Full-text

Identification and sequence analysis of a new member of the mouse HSP70 gene family and characterization of its unique cellular and developmental pattern of expression in the male germ line

Molecular and Cellular Biology ◽

10.1128/mcb.8.7.2925-2932.1988 ◽

1988 ◽

Vol 8 (7) ◽

pp. 2925-2932

Author(s):

Z F Zakeri ◽

D J Wolgemuth ◽

C R Hunt

Keyword(s):

Heat Shock ◽

Gene Family ◽

Developmental Stages ◽

Germ Line ◽

Cell Lineage ◽

Hsp70 Gene ◽

Coding Region ◽

Male Germ Line ◽

Sequence Organization

A unique member of the mouse HSP70 gene family has been isolated and characterized with respect to its DNA sequence organization and expression. The gene contains extensive similarity to a heat shock-inducible HSP70 gene within the coding region but diverges in both 3' and 5' nontranslated regions. The gene does not yield transcripts in response to heat shock in mouse L cells. Rather, the gene appears to be activated uniquely in the male germ line. Analysis of RNA from different developmental stages and from enriched populations of spermatogenic cells revealed that this gene is expressed during the prophase stage of meiosis. A transcript different in size from the major heat-inducible mouse transcripts is most abundant in meiotic prophase spermatocytes and decreases in abundance in postmeiotic stages of spermatogenesis. This pattern of expression is distinct from that observed for another member of this gene family, which was previously shown to be expressed abundantly in postmeiotic germ cells. These observations suggest that specific HSP70 gene family members play distinct roles in the differentiation of the germ cell lineage in mammals.

Download Full-text