scholarly journals Meiotic recombination between dispersed repeated genes is associated with heteroduplex formation

1990 ◽  
Vol 10 (8) ◽  
pp. 4420-4423
Author(s):  
D K Nag ◽  
T D Petes
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Meiotic Recombination ◽  
Homologous Chromosomes ◽  
Ectopic Recombination ◽  
Heteroduplex Formation ◽  
Repeated Genes

In Saccharomyces cerevisiae, recombination events occurring between allelic genes located on homologous chromosomes are often associated with heteroduplex formation. We found that recombination events between repeated genes on nonhomologous chromosomes (ectopic events) are also associated with the formation of heteroduplexes, indicating that classical and ectopic recombination events involve similar mechanisms.

scholarly journals Meiotic recombination between dispersed repeated genes is associated with heteroduplex formation.

1990 ◽  
Vol 10 (8) ◽  
pp. 4420-4423 ◽  
Author(s):  
D K Nag ◽  
T D Petes
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Meiotic Recombination ◽  
Homologous Chromosomes ◽  
Ectopic Recombination ◽  
Heteroduplex Formation ◽  
Repeated Genes

In Saccharomyces cerevisiae, recombination events occurring between allelic genes located on homologous chromosomes are often associated with heteroduplex formation. We found that recombination events between repeated genes on nonhomologous chromosomes (ectopic events) are also associated with the formation of heteroduplexes, indicating that classical and ectopic recombination events involve similar mechanisms.

Analysis of a gene conversion gradient at the HIS4 locus in Saccharomyces cerevisiae.

Genetics ◽  
1992 ◽  
Vol 132 (1) ◽  
pp. 113-123 ◽  
Author(s):  
P Detloff ◽  
M A White ◽  
T D Petes
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gene Conversion ◽  
Mismatch Repair ◽  
Meiotic Recombination ◽  
Present Evidence ◽  
Homologous Chromosomes ◽  
A Site ◽  
His4 Gene ◽  
Heteroduplex Formation ◽  
Recombination Gene

Abstract Heteroduplexes formed between genes on homologous chromosomes are intermediates in meiotic recombination. In the HIS4 gene of Saccharomyces cerevisiae, most mutant alleles at the 5' end of the gene have a higher rate of meiotic recombination (gene conversion) than mutant alleles at the 3' end of the gene. Such gradients are usually interpreted as indicating a higher frequency of heteroduplex formation at the high conversion end of the gene. We present evidence indicating that the gradient of conversion at HIS4 primarily reflects the direction of mismatch repair rather than the frequency of heteroduplex formation. We also identify a site located between the 5' end of HIS4 and the 3' end of BIK1 that stimulates heteroduplex formation at HIS4 and BIK1.

scholarly journals CHROMOSOMAL TRANSLOCATIONS GENERATED BY HIGH-FREQUENCY MEIOTIC RECOMBINATION BETWEEN REPEATED YEAST GENES

Genetics ◽  
1986 ◽  
Vol 114 (3) ◽  
pp. 731-752
Author(s):  
Sue Jinks-Robertson ◽  
Thomas D Petes
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gene Conversion ◽  
Meiotic Recombination ◽  
High Frequency ◽  
Mitotic Recombination ◽  
Chromosomal Translocations ◽  
Yeast Saccharomyces Cerevisiae ◽  
Homologous Chromosomes ◽  
Yeast Genes ◽  
Repeated Genes

ABSTRACT We have examined meiotic and mitotic recombination between repeated genes on nonhomologous chromosomes in the yeast Saccharomyces cerevisiae . The results of these experiments can be summarized in three statements. First, gene conversion events between repeats on nonhomologous chromosomes occur frequently in meiosis. The frequency of such conversion events is only 17-fold less than the analogous frequency of conversion between genes at allelic positions on homologous chromosomes. Second, meiotic and mitotic conversion events between repeated genes on nonhomologous chromosomes are associated with reciprocal recombination to the same extent as conversion between allelic sequences. The reciprocal exchanges between the repeated genes result in chromosomal translocations. Finally, recombination between repeated genes on nonhomologous chromosomes occurs much more frequently in meiosis than in mitosis.

scholarly journals The Efficiency of Meiotic Recombination Between Dispersed Sequences in Saccharomyces cerevisiae Depends Upon Their Chromosomal Location

Genetics ◽  
1996 ◽  
Vol 144 (1) ◽  
pp. 43-55 ◽  
Author(s):  
Alastair S H Goldman ◽  
Michael Lichten
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Meiotic Recombination ◽  
Chromosomal Location ◽  
Physical Distance ◽  
Special Relationship ◽  
Homologous Chromosomes ◽  
Ectopic Recombination ◽  
End To End

Abstract To examine constraints imposed on meiotic recombination by homologue pairing, we measured the frequency of recombination between mutant alleles of the ARG4 gene contained in pBR322-based inserts. Inserts were located at identical loci on homologues (allelic recombination) or at different loci on either homologous or heterologous chromosomes (ectopic recombination). Ectopic recombination between interstitially located inserts on heterologous chromosomes had an efficiency of 6-12% compared to allelic recombination. By contrast, ectopic recombination between interstitial inserts located on homologues had relative efficiencies of 47-99%. These findings suggest that when meiotic ectopic recombination occurs, homologous chromosomes are already colocalized. The efficiency of ectopic recombination between inserts on homologues decreased as the physical distance between insert sites was increased. This result is consistent with the suggestion that during meiotic recombination, homologues are not only close to each other, but also are aligned end to end. Finally, the efficiency of ectopic recombination between inserts near telomeres (within 16 kb) was significantly greater than that observed with inserts >50 kb from the nearest telomere. Thus, at the time of recombination, there may be a special relationship between the ends of chromosomes not shared with interstitial regions.

scholarly journals Physical detection of heteroduplexes during meiotic recombination in the yeast Saccharomyces cerevisiae.

1993 ◽  
Vol 13 (4) ◽  
pp. 2324-2331 ◽  
Author(s):  
D K Nag ◽  
T D Petes
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Meiotic Recombination ◽  
Physical Method ◽  
Yeast Saccharomyces Cerevisiae ◽  
Heteroduplex Dna ◽  
Dna Strands ◽  
General Physical ◽  
Palindromic Sequences ◽  
Heteroduplex Formation

We describe a general physical method for detecting the heteroduplex DNA that is formed as an intermediate in meiotic recombination in the yeast Saccharomyces cerevisiae. We use this method to study the kinetic relationship between the formation of heteroduplex DNA and other meiotic events. We show that strains with the rad50, but not the rad52, mutation are defective in heteroduplex formation. We also demonstrate that, although cruciform structures can be formed in vivo as a consequence of heteroduplex formation between DNA strands that contain different palindromic insertions, small palindromic sequences in homoduplex DNA are rarely extruded into the cruciform conformation.

scholarly journals RAD10, an excision repair gene of Saccharomyces cerevisiae, is involved in the RAD1 pathway of mitotic recombination.

1990 ◽  
Vol 10 (6) ◽  
pp. 2485-2491 ◽  
Author(s):  
R H Schiestl ◽  
S Prakash
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Meiotic Recombination ◽  
Excision Repair ◽  
Mitotic Recombination ◽  
The Other ◽  
Gene Products ◽  
Repair Gene ◽  
Homologous Chromosomes ◽  
Intrachromosomal Recombination ◽  
Recombination Pathway

The RAD10 gene of Saccharomyces cerevisiae is required for the incision step of excision repair of UV-damaged DNA. We show that the RAD10 gene is also required for mitotic recombination. The rad10 delta mutation lowered the rate of intrachromosomal recombination of a his3 duplication in which one his3 allele has a deletion at the 3' end and the other his3 allele has a deletion at the 5' end (his3 delta 3' his3 delta 5'). The rate of formation of HIS3+ recombinants in the rad10 delta mutant was not affected by the rad1 delta mutation but decreased synergistically in the presence of the rad10 delta mutation in combination with the rad52 delta mutation. These observations indicate that the RAD1 and RAD10 genes function together in a mitotic recombination pathway that is distinct from the RAD52 recombination pathway. The rad10 delta mutation also lowered the efficiency of integration of linear DNA molecules and circular plasmids into homologous genomic sequences. We suggest that the RAD1 and RAD10 gene products act in recombination after the formation of the recombinogenic substrate. The rad1 delta and rad10 delta mutations did not affect meiotic intrachromosomal recombination of the his3 delta 3' his3 delta 5' duplication or mitotic and meiotic recombination of ade2 heteroalleles located on homologous chromosomes.

scholarly journals Genome Duplication Increases Meiotic Recombination Frequency: A Saccharomyces cerevisiae Model

2020 ◽  
Author(s):  
Ou Fang ◽  
Lin Wang ◽  
Yuxin Zhang ◽  
Jixuan Yang ◽  
Qin Tao ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Meiotic Recombination ◽  
Genome Stability ◽  
Genome Duplication ◽  
Genetic Recombination ◽  
Recombination Frequency ◽  
Abiotic Factors ◽  
Strand Break ◽  
Homologous Chromosomes ◽  
Almost All

Abstract Genetic recombination characterized by reciprocal exchange of genes on paired homologous chromosomes is the most prominent event in meiosis of almost all sexually reproductive organisms. It contributes to genome stability by ensuring the balanced segregation of paired homologs in meiosis, and it is also the major driving factor in generating genetic variation for natural and artificial selection. Meiotic recombination is subjected to the control of a highly stringent and complex regulating process and meiotic recombination frequency (MRF) may be affected by biological and abiotic factors such as sex, gene density, nucleotide content, and chemical/temperature treatments, having motivated tremendous researches for artificially manipulating MRF. Whether genome polyploidization would lead to a significant change in MRF has attracted both historical and recent research interests; however, tackling this fundamental question is methodologically challenging due to the lack of appropriate methods for tetrasomic genetic analysis, thus has led to controversial conclusions in the literature. This article presents a comprehensive and rigorous survey of genome duplication-mediated change in MRF using Saccharomyces cerevisiae as a eukaryotic model. It demonstrates that genome duplication can lead to consistently significant increase in MRF and rate of crossovers across all 16 chromosomes of S. cerevisiae, including both cold and hot spots of MRF. This ploidy-driven change in MRF is associated with weakened recombination interference, enhanced double-strand break density, and loosened chromatin histone occupation. The study illuminates a significant evolutionary feature of genome duplication and opens an opportunity to accelerate response to artificial and natural selection through polyploidization.

scholarly journals Measurements of excision repair tracts formed during meiotic recombination in Saccharomyces cerevisiae

1992 ◽  
Vol 12 (4) ◽  
pp. 1805-1814
Author(s):  
P Detloff ◽  
T D Petes
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Meiotic Recombination ◽  
High Frequency ◽  
Excision Repair ◽  
Yeast Saccharomyces Cerevisiae ◽  
Heteroduplex Dna ◽  
Homologous Chromosomes ◽  
His4 Gene

During meiotic recombination in the yeast Saccharomyces cerevisiae, heteroduplexes are formed at a high frequency between HIS4 genes located on homologous chromosomes. Using mutant alleles of the HIS4 gene that result in poorly repaired mismatches in heteroduplex DNA, we find that heteroduplexes often span a distance of 1.8 kb. In addition, we show that about one-third of the repair tracts initiated at well-repaired mismatches extend 900 bp.

scholarly journals Physical detection of heteroduplexes during meiotic recombination in the yeast Saccharomyces cerevisiae

1993 ◽  
Vol 13 (4) ◽  
pp. 2324-2331 ◽  
Author(s):  
D K Nag ◽  
T D Petes
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Meiotic Recombination ◽  
Physical Method ◽  
Yeast Saccharomyces Cerevisiae ◽  
Heteroduplex Dna ◽  
Dna Strands ◽  
General Physical ◽  
Palindromic Sequences ◽  
Heteroduplex Formation

We describe a general physical method for detecting the heteroduplex DNA that is formed as an intermediate in meiotic recombination in the yeast Saccharomyces cerevisiae. We use this method to study the kinetic relationship between the formation of heteroduplex DNA and other meiotic events. We show that strains with the rad50, but not the rad52, mutation are defective in heteroduplex formation. We also demonstrate that, although cruciform structures can be formed in vivo as a consequence of heteroduplex formation between DNA strands that contain different palindromic insertions, small palindromic sequences in homoduplex DNA are rarely extruded into the cruciform conformation.

RAD10, an excision repair gene of Saccharomyces cerevisiae, is involved in the RAD1 pathway of mitotic recombination

1990 ◽  
Vol 10 (6) ◽  
pp. 2485-2491
Author(s):  
R H Schiestl ◽  
S Prakash
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Meiotic Recombination ◽  
Excision Repair ◽  
Mitotic Recombination ◽  
The Other ◽  
Gene Products ◽  
Repair Gene ◽  
Homologous Chromosomes ◽  
Intrachromosomal Recombination ◽  
Recombination Pathway

The RAD10 gene of Saccharomyces cerevisiae is required for the incision step of excision repair of UV-damaged DNA. We show that the RAD10 gene is also required for mitotic recombination. The rad10 delta mutation lowered the rate of intrachromosomal recombination of a his3 duplication in which one his3 allele has a deletion at the 3' end and the other his3 allele has a deletion at the 5' end (his3 delta 3' his3 delta 5'). The rate of formation of HIS3+ recombinants in the rad10 delta mutant was not affected by the rad1 delta mutation but decreased synergistically in the presence of the rad10 delta mutation in combination with the rad52 delta mutation. These observations indicate that the RAD1 and RAD10 genes function together in a mitotic recombination pathway that is distinct from the RAD52 recombination pathway. The rad10 delta mutation also lowered the efficiency of integration of linear DNA molecules and circular plasmids into homologous genomic sequences. We suggest that the RAD1 and RAD10 gene products act in recombination after the formation of the recombinogenic substrate. The rad1 delta and rad10 delta mutations did not affect meiotic intrachromosomal recombination of the his3 delta 3' his3 delta 5' duplication or mitotic and meiotic recombination of ade2 heteroalleles located on homologous chromosomes.

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