scholarly journals TFIIA induces conformational changes in TFIID via interactions with the basic repeat.

1992 ◽  
Vol 12 (11) ◽  
pp. 5189-5196 ◽  
Author(s):  
D K Lee ◽  
J DeJong ◽  
S Hashimoto ◽  
M Horikoshi ◽  
R G Roeder
Keyword(s):  
Dna Binding ◽  
Conformational Changes ◽  
Structural Changes ◽  
Inhibitory Effect ◽  
Binding Studies ◽  
Binding Interaction ◽  
Contact Points ◽  
Tata Element ◽  
N Terminus ◽  
Dna Binding Studies

DNA-binding studies with Saccharomyces cerevisiae TFIID point mutants indicated that TFIIA interacts with the basic repeat region of TFIID and induces structural changes. The latter was shown by the ability of TFIIA to compensate for TFIID point mutants defective for DNA binding. Interaction with TFIIA also rendered TFIID binding temperature independent, thus mimicking the effect of removing the nonconserved N terminus of TFIID. In addition, N-terminal truncation of the TFIID point mutants defective for DNA binding mimicked the ability of TFIIA to restore DNA binding of those mutants. Taken together, these results suggest that TFIIA enhances TFIID binding to DNA by eliminating an otherwise inhibitory effect of the nonconserved N terminus of TFIID. Furthermore, analyses of TFIID contact points on DNA and binding studies with TATA-containing oligonucleotide probes showed that TFIIA decreases the effect of sequences flanking the adenovirus major late TATA element on TFIID binding to DNA, suggesting a possible role of TFIIA in allowing TFIID to recognize a wider variety of promoters.

TFIIA induces conformational changes in TFIID via interactions with the basic repeat

1992 ◽  
Vol 12 (11) ◽  
pp. 5189-5196 ◽  
Author(s):  
D K Lee ◽  
J DeJong ◽  
S Hashimoto ◽  
M Horikoshi ◽  
R G Roeder
Keyword(s):  
Dna Binding ◽  
Conformational Changes ◽  
Structural Changes ◽  
Inhibitory Effect ◽  
Binding Studies ◽  
Binding Interaction ◽  
Contact Points ◽  
Tata Element ◽  
N Terminus ◽  
Dna Binding Studies

DNA-binding studies with Saccharomyces cerevisiae TFIID point mutants indicated that TFIIA interacts with the basic repeat region of TFIID and induces structural changes. The latter was shown by the ability of TFIIA to compensate for TFIID point mutants defective for DNA binding. Interaction with TFIIA also rendered TFIID binding temperature independent, thus mimicking the effect of removing the nonconserved N terminus of TFIID. In addition, N-terminal truncation of the TFIID point mutants defective for DNA binding mimicked the ability of TFIIA to restore DNA binding of those mutants. Taken together, these results suggest that TFIIA enhances TFIID binding to DNA by eliminating an otherwise inhibitory effect of the nonconserved N terminus of TFIID. Furthermore, analyses of TFIID contact points on DNA and binding studies with TATA-containing oligonucleotide probes showed that TFIIA decreases the effect of sequences flanking the adenovirus major late TATA element on TFIID binding to DNA, suggesting a possible role of TFIIA in allowing TFIID to recognize a wider variety of promoters.

scholarly journals Synthesis and DNA-Binding Studies of a Dinuclear Gadolinium(III)–Platinum(II) Complex

2015 ◽  
Vol 68 (4) ◽  
pp. 576 ◽  
Author(s):  
Jacob M. Fenton ◽  
Madleen Busse ◽  
Louis M. Rendina
Keyword(s):  
Dna Binding ◽  
Linear Dichroism ◽  
Binding Mode ◽  
In Vitro Cytotoxicity ◽  
Binding Studies ◽  
Binding Interaction ◽  
Absorption Region ◽  
Synthetic Strategy ◽  
Dna Binding Studies

The synthesis and characterisation of a new dinuclear GdIII–PtII complex (1·PF6) containing a functionalised macrocyclic 1,4,7,10-tetraazacyclododecane-1,4,7-triacetic acid derivative linked to a PtII-terpy (terpy = 2,2′ : 6′,2″-terpyridine) unit by means of a short thiolato linker are reported. The complex was synthesised in six steps from cyclen by means of a modular synthetic strategy. A preliminary DNA-binding study with calf-thymus DNA (ct-DNA) was performed on 1·PF6 by means of linear dichroism (LD). The observed changes in the DNA LD signal in the presence of the metal complex are fully consistent with an intercalative binding mode. Furthermore, an induced negative LD signal in the ultraviolet absorption region of the complex provides strong evidence of a strong DNA-binding interaction. The in vitro cytotoxicity of 1·PF6 towards a human glioblastoma cell line (T98G) was also determined.

Towards understanding the mechanism of action of mithramycin and its analogues: dimerization and DNA binding studies

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
S Weidenbach ◽  
C Hou ◽  
JM Chen ◽  
OV Tsodikov ◽  
J Rohr
Keyword(s):  
Dna Binding ◽  
Mechanism Of Action ◽  
Binding Studies ◽  
Dna Binding Studies

Metal Based Pharmacological Active Agents: Structural, Electrochemical, DNA Binding Studies and Antibacterial Properties

2015 ◽  
Vol 9 (4) ◽  
pp. 210-218 ◽  
Author(s):  
Harun Muslu ◽  
Derya Kılıcaslan ◽  
Mustafa Cesme ◽  
Aysegul Golcu ◽  
Sibel Ozkan
Keyword(s):  
Dna Binding ◽  
Antibacterial Properties ◽  
Binding Studies ◽  
Dna Binding Studies

Arene ruthenium, rhodium and iridium complexes containing benzamide derivative ligands: Study of interesting bonding modes, antibacterial, antioxidant and DNA binding studies

2021 ◽  
Vol 937 ◽  
pp. 121731
Author(s):  
Lathewdeipor Shadap ◽  
Nipanshu Agarwal ◽  
Vivek Chetry ◽  
Krishna Mohan Poluri ◽  
Werner Kaminsky ◽  
...  
Keyword(s):  
Dna Binding ◽  
Iridium Complexes ◽  
Binding Studies ◽  
Dna Binding Studies

DNA-binding studies and antioxidant activities of two-, three- and four-coordinate silver(i) complexes containing bis(2-benzimidazolyl)aniline derivatives

2014 ◽  
Vol 38 (8) ◽  
pp. 3688-3698 ◽  
Author(s):  
Huilu Wu ◽  
Yanhui Zhang ◽  
Chengyong Chen ◽  
Jiawen Zhang ◽  
Yuchen Bai ◽  
...  
Keyword(s):  
Dna Binding ◽  
Hydroxyl Radical ◽  
Superoxide Radical ◽  
Antioxidant Activities ◽  
Binding Studies ◽  
Aniline Derivatives ◽  
Novel Structure ◽  
Dna Binding Studies

Three novel structure Ag(i) complexes have been synthesized and characterized. The three ligands and Ag(i) complexes bind to DNA in an intercalation mode. The Ag(i) complexes have stronger ability of antioxidation for hydroxyl radical and superoxide radical.

DNA binding studies of a solvatochromic fluorescence probe 3-methoxybenzanthrone

1999 ◽  
Vol 55 (13) ◽  
pp. 2719-2727 ◽  
Author(s):  
Xin Yang ◽  
Wan-Hui Liu ◽  
Wei-Jun Jin ◽  
Guo-Li Shen ◽  
Ru-Qin Yu
Keyword(s):  
Dna Binding ◽  
Fluorescence Probe ◽  
Binding Studies ◽  
Dna Binding Studies
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