Synthesis and DNA-Binding Studies of a Dinuclear Gadolinium(III)–Platinum(II) Complex

The synthesis and characterisation of a new dinuclear GdIII–PtII complex (1·PF6) containing a functionalised macrocyclic 1,4,7,10-tetraazacyclododecane-1,4,7-triacetic acid derivative linked to a PtII-terpy (terpy = 2,2′ : 6′,2″-terpyridine) unit by means of a short thiolato linker are reported. The complex was synthesised in six steps from cyclen by means of a modular synthetic strategy. A preliminary DNA-binding study with calf-thymus DNA (ct-DNA) was performed on 1·PF6 by means of linear dichroism (LD). The observed changes in the DNA LD signal in the presence of the metal complex are fully consistent with an intercalative binding mode. Furthermore, an induced negative LD signal in the ultraviolet absorption region of the complex provides strong evidence of a strong DNA-binding interaction. The in vitro cytotoxicity of 1·PF6 towards a human glioblastoma cell line (T98G) was also determined.

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Synthesis, characterization, DNA binding studies and in vitro cytotoxicity of platinum(II)-dihalogenido complexes containing bidentate chelating N-donor ligands

Journal of Coordination Chemistry ◽

10.1080/00958972.2016.1199862 ◽

2016 ◽

Vol 69 (16) ◽

pp. 2422-2436 ◽

Cited By ~ 11

Author(s):

Radka Křikavová ◽

Ján Vančo ◽

Tomáš Šilha ◽

Jaromír Marek ◽

Zdeněk Trávníček

Keyword(s):

Dna Binding ◽

In Vitro Cytotoxicity ◽

Donor Ligands ◽

Binding Studies ◽

Dna Binding Studies

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IN VITRO DNA BINDING STUDIES OF SELECTED HETEROCYCLIC COMPOUNDS

INDIAN DRUGS ◽

10.53879/id.55.12.10994 ◽

2018 ◽

Vol 55 (12) ◽

pp. 24-26

Author(s):

C Akhila ◽

◽

P Lalitha

Keyword(s):

Dna Binding ◽

Heterocyclic Compounds ◽

Uv Spectroscopy ◽

Binding Mode ◽

Spectroscopic Technique ◽

Binding Studies ◽

Base Pairs ◽

Dna Base ◽

Dna Binding Studies

DNA binding studies of selected heterocyclic compounds belonging to the class of quinolinones, substituted quinolinones and thiones were carried out using ct-DNA. The binding nature of the compounds with DNA analyzed using UV-spectroscopy revealed the compounds to be DNA intercalators demonstrating the binding nature of compounds with DNA base pairs. This study is aimed at establishing a facile UV spectroscopic technique to arrive at the binding mode of DNA to ligands.

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In Vitro Cytotoxicity and DNA Binding Studies of Newly Synthesized Oxidovanadium (IV) Complexes of Nitro-Substituted Benzohydroxamate Ligands as Prospective Vanadodrug Compounds

Advanced Science Engineering and Medicine ◽

10.1166/asem.2018.2095 ◽

2018 ◽

Vol 10 (1) ◽

pp. 27-37 ◽

Cited By ~ 2

Author(s):

Abhishek Kumar ◽

Bhanu Priya ◽

Archana Thakur ◽

Neeraj Sharma

Keyword(s):

Dna Binding ◽

In Vitro Cytotoxicity ◽

Binding Studies ◽

Dna Binding Studies

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Carbohydrate-conjugate heterobimetallic complexes: synthesis, DNA binding studies, artificial nuclease activity and in vitro cytotoxicity

Carbohydrate Research ◽

10.1016/j.carres.2011.10.010 ◽

2011 ◽

Vol 346 (18) ◽

pp. 2886-2895 ◽

Cited By ~ 17

Author(s):

Sartaj Tabassum ◽

Rais Ahmad Khan ◽

Farukh Arjmand ◽

Mubashira Aziz ◽

Aarti S. Juvekar ◽

...

Keyword(s):

Dna Binding ◽

Nuclease Activity ◽

In Vitro Cytotoxicity ◽

Binding Studies ◽

Heterobimetallic Complexes ◽

Dna Binding Studies ◽

Artificial Nuclease

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Synthesis of new steroidal imidazo [1,2-a] pyridines: DNA binding studies, cleavage activity and in vitro cytotoxicity

Steroids ◽

10.1016/j.steroids.2015.09.010 ◽

2015 ◽

Vol 104 ◽

pp. 163-175 ◽

Cited By ~ 11

Author(s):

Ayaz Mahmood Dar ◽

Shamsuzzaman ◽

Manzoor Ahmad Gatoo

Keyword(s):

Dna Binding ◽

In Vitro Cytotoxicity ◽

Binding Studies ◽

Cleavage Activity ◽

Dna Binding Studies

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TFIIA induces conformational changes in TFIID via interactions with the basic repeat.

Molecular and Cellular Biology ◽

10.1128/mcb.12.11.5189 ◽

1992 ◽

Vol 12 (11) ◽

pp. 5189-5196 ◽

Cited By ~ 65

Author(s):

D K Lee ◽

J DeJong ◽

S Hashimoto ◽

M Horikoshi ◽

R G Roeder

Keyword(s):

Dna Binding ◽

Conformational Changes ◽

Structural Changes ◽

Inhibitory Effect ◽

Binding Studies ◽

Binding Interaction ◽

Contact Points ◽

Tata Element ◽

N Terminus ◽

Dna Binding Studies

DNA-binding studies with Saccharomyces cerevisiae TFIID point mutants indicated that TFIIA interacts with the basic repeat region of TFIID and induces structural changes. The latter was shown by the ability of TFIIA to compensate for TFIID point mutants defective for DNA binding. Interaction with TFIIA also rendered TFIID binding temperature independent, thus mimicking the effect of removing the nonconserved N terminus of TFIID. In addition, N-terminal truncation of the TFIID point mutants defective for DNA binding mimicked the ability of TFIIA to restore DNA binding of those mutants. Taken together, these results suggest that TFIIA enhances TFIID binding to DNA by eliminating an otherwise inhibitory effect of the nonconserved N terminus of TFIID. Furthermore, analyses of TFIID contact points on DNA and binding studies with TATA-containing oligonucleotide probes showed that TFIIA decreases the effect of sequences flanking the adenovirus major late TATA element on TFIID binding to DNA, suggesting a possible role of TFIIA in allowing TFIID to recognize a wider variety of promoters.

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