scholarly journals Ligand-induced assembly and activation of the gamma interferon receptor in intact cells.

1996 ◽  
Vol 16 (6) ◽  
pp. 3214-3221 ◽  
Author(s):  
E A Bach ◽  
J W Tanner ◽  
S Marsters ◽  
A Ashkenazi ◽  
M Aguet ◽  
...  
Keyword(s):  
Gamma Interferon ◽  
Beta Chain ◽  
Ifn Gamma ◽  
Intracellular Domain ◽  
Receptor Alpha ◽  
Alpha Chain ◽  
Gamma Receptor ◽  
Receptor Beta ◽  
Receptor Alpha Chain

Functionally active gamma interferon (IFN-gamma) receptors consist of an alpha subunit required for ligand binding and signal transduction and a beta subunit required primarily for signaling. Although the receptor alpha chain has been well characterized, little is known about the specific role of the receptor beta chain in IFN-gamma signaling. Expression of the wild-type human IFN-gamma receptor beta chain in murine L cells that stably express the human IFN-gamma receptor alpha chain (L.hgR) produced a murine cell line (L.hgR.myc beta) that responded to human IFN-gamma. Mutagenesis of the receptor beta-chain intracellular domain revealed that only two closely spaced, membrane-proximal sequences (P263PSIP267 and I270EEYL274) are required for function. Coprecipitation studies showed that these sequences are necessary for the specific and constitutive association of the receptor beta chain with the JAK-2 tyrosine kinase. These experiments also revealed that the IFN-gamma receptor alpha and beta chains are not preassociated on the surface of unstimulated cells but rather are induced to associate in an IFN-gamma-dependent fashion. A chimeric protein in which the intracellular domain of the beta chain was replaced by JAK-2 complemented human IFN-gamma signaling and biologic responsiveness in L.hgR. In contrast, a c-src-containing beta-chain chimera did not. These results indicate that the sole obligate role of the IFN-gamma receptor beta chain in signaling is to recruit JAK-2 into the ligand-assembled receptor complex.

Ligand-Induced Autoregulation of IFN-gamma Receptor beta Chain Expression in T Helper Cell Subsets

Science ◽  
1995 ◽  
Vol 270 (5239) ◽  
pp. 1215-1218 ◽  
Author(s):  
E. A. Bach ◽  
S. J. Szabo ◽  
A. S. Dighe ◽  
A. Ashkenazi ◽  
M. Aguet ◽  
...  
Keyword(s):  
T Helper Cell ◽  
Helper Cell ◽  
Beta Chain ◽  
T Helper ◽  
Ifn Gamma ◽  
Gamma Receptor ◽  
Receptor Beta

scholarly journals Cloning of rat interleukin 11 and interleukin 11 receptor alpha chain and analysis of their expression in rat uterus in the peri-implantation period

Reproduction ◽  
2001 ◽  
pp. 593-600 ◽  
Author(s):  
R Li ◽  
L Hartley ◽  
L Robb
Keyword(s):  
Rat Uterus ◽  
Rnase Protection ◽  
Receptor Alpha ◽  
Alpha Chain ◽  
Genes Encoding ◽  
Interleukin 11 ◽  
Implantation Period ◽  
Receptor Alpha Chain

Studies in mice have shown that interleukin 11 (IL-11) signalling is required for female fertility. In the absence of IL-11, decidualization is markedly retarded and implantation fails. IL-11 acts via a heterodimeric receptor composed of a ligand-specific receptor alpha chain (IL-11R alpha) and the signalling moiety gp130. This study reports the cloning of genes encoding rat IL-11 and IL-11R alpha. RNase protection was used to demonstrate that expression of IL-11 is upregulated in the rat uterus at the initiation of implantation at 5.5 days after mating. Expression of the genes encoding the two receptor components, IL11Ra and gp130, did not change throughout the peri-implantation period. In situ hybridization studies revealed that, as in mice, expression of IL-11 was high in the primary decidual zone at the time of the attachment reaction, whereas IL11Ra was expressed throughout primary and secondary decidua. Conservation of the temporal and spatial expression of IL-11 and IL-11R alpha in the uterus of the mouse and rat during the peri-implantation period will facilitate future studies investigating the role of IL-11 in fertility.

scholarly journals Phenotypic analysis of hairy cell leukemia: "variant" cases express the interleukin-2 receptor beta chain, but not the alpha chain (CD25)

Blood ◽  
1993 ◽  
Vol 82 (2) ◽  
pp. 528-535
Author(s):  
D de Totero ◽  
PL Tazzari ◽  
F Lauria ◽  
D Raspadori ◽  
PF di Celle ◽  
...  
Keyword(s):  
B Cell ◽  
Hairy Cell Leukemia ◽  
Interleukin 2 ◽  
Variant Form ◽  
Hairy Cell ◽  
Beta Chain ◽  
Cell Leukemia ◽  
Receptor Alpha ◽  
Alpha Chain ◽  
Receptor Beta

Hairy cell leukemia (HCL) is a B-cell chronic lymphoproliferative disorder in which the pathologic cells show a strong expression of CD25 (interleukin-2 [IL-2] receptor alpha chain or p55). “Variant” cases of HCL, characterized by hyperleukocytosis, neoplastic elements with a prominent nucleolus and a higher nucleo/cytoplasmic ratio, and an easily obtained bone marrow aspirate, lack surface CD25 determinants. Limited information is available on the expression of the IL-2 receptor beta chain (p75) on normal and neoplastic B cells. In this study, we have assessed by immunofluorescence and mRNA analysis the presence of the IL-2 receptor alpha and beta chains on 12 cases of classic HCL, as well as on 3 variant cases. The results obtained show that, while the alpha chain of the IL-2 receptor is present only on classic HCL, the IL- 2 receptor beta chain (p75) is expressed on both the classic and variant form. Unlike hairy cells, only 8 of the 15 B-cell chronic lymphocytic leukemia cases tested showed a weak expression of the p75 antigen on a small proportion of cells. Purified B lymphocytes from normal healthy controls, as well as Epstein-Barr virus-transformed lymphoblastoid cell lines, showed a weak staining for the p75 determinant, while being CD25-. The results of this study suggest that the expression of the alpha and beta chains of the IL-2 receptor appears to be upregulated or downregulated during the process of B-cell- lineage activation and differentiation.

scholarly journals A cytokine receptor gene cluster in the X-Y pseudoautosomal region?

Blood ◽  
1993 ◽  
Vol 82 (1) ◽  
pp. 22-28 ◽  
Author(s):  
E Kremer ◽  
E Baker ◽  
RJ D'Andrea ◽  
R Slim ◽  
H Phillips ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Restriction Fragment ◽  
Sex Chromosomes ◽  
Receptor Gene ◽  
Pseudoautosomal Region ◽  
Beta Chain ◽  
Receptor Alpha ◽  
Alpha Chain ◽  
Gm Csf ◽  
Receptor Alpha Chain

The receptors for interleukin-3 (IL-3), IL-5, and granulocyte- macrophage colony-stimulating factor (GM-CSF) are heterodimers comprised of ligand specific alpha chains and a common beta chain. The genes encoding the IL-5 receptor alpha chain and the common beta chain reside on chromosome 3 and 22 respectively, while the GM-CSF receptor alpha chain gene (CSF2RA) has been mapped to the pseudoautosomal region (PAR) of the sex chromosomes, which is a 2.6-Mb stretch of homologous sequence at the tips of the short arms within which a single obligatory recombination occurs during male meiosis. We have mapped the gene encoding the IL-3 receptor alpha chain (IL3RA) to the sex chromosomes by polymerase chain reaction (PCR) analysis of human-mouse or human- chinese hamster cell hybrids, and to Yp13.3 and Xp22.3 using fluorescence in situ hybridization. To explore the possibility that IL3RA is located within the pseudoautosomal region we screened the Centre d'Etude du Polymorphisme Humain (CEPH) pedigrees for an informative-restriction fragment-length polymorphism (RFLP) that showed male meiotic recombination. Two informative CEPH pedigrees were identified that displayed this phenomenon, confirming the psuedoautosomal location of IL3RA. Using long-range restriction mapping we have found that IL3RA maps to the same 190-kb restriction fragment as CSF2RA, suggesting that a cytokine receptor gene cluster may reside in the PAR.

scholarly journals Phenotypic analysis of hairy cell leukemia: "variant" cases express the interleukin-2 receptor beta chain, but not the alpha chain (CD25)

Blood ◽  
1993 ◽  
Vol 82 (2) ◽  
pp. 528-535 ◽  
Author(s):  
D de Totero ◽  
PL Tazzari ◽  
F Lauria ◽  
D Raspadori ◽  
PF di Celle ◽  
...  
Keyword(s):  
B Cell ◽  
Hairy Cell Leukemia ◽  
Interleukin 2 ◽  
Variant Form ◽  
Hairy Cell ◽  
Beta Chain ◽  
Cell Leukemia ◽  
Receptor Alpha ◽  
Alpha Chain ◽  
Receptor Beta

Abstract Hairy cell leukemia (HCL) is a B-cell chronic lymphoproliferative disorder in which the pathologic cells show a strong expression of CD25 (interleukin-2 [IL-2] receptor alpha chain or p55). “Variant” cases of HCL, characterized by hyperleukocytosis, neoplastic elements with a prominent nucleolus and a higher nucleo/cytoplasmic ratio, and an easily obtained bone marrow aspirate, lack surface CD25 determinants. Limited information is available on the expression of the IL-2 receptor beta chain (p75) on normal and neoplastic B cells. In this study, we have assessed by immunofluorescence and mRNA analysis the presence of the IL-2 receptor alpha and beta chains on 12 cases of classic HCL, as well as on 3 variant cases. The results obtained show that, while the alpha chain of the IL-2 receptor is present only on classic HCL, the IL- 2 receptor beta chain (p75) is expressed on both the classic and variant form. Unlike hairy cells, only 8 of the 15 B-cell chronic lymphocytic leukemia cases tested showed a weak expression of the p75 antigen on a small proportion of cells. Purified B lymphocytes from normal healthy controls, as well as Epstein-Barr virus-transformed lymphoblastoid cell lines, showed a weak staining for the p75 determinant, while being CD25-. The results of this study suggest that the expression of the alpha and beta chains of the IL-2 receptor appears to be upregulated or downregulated during the process of B-cell- lineage activation and differentiation.

Role of the T cell receptor alpha chain in the development and phenotype of naturally arising CD4CD25 T cells

2006 ◽  
Vol 43 (3) ◽  
pp. 246-254 ◽  
Author(s):  
N BOSCO ◽  
H HUNG ◽  
N PASQUAL ◽  
E JOUVINMARCHE ◽  
P MARCHE ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Receptor Alpha ◽  
Alpha Chain ◽  
Receptor Alpha Chain ◽  
Cell Receptor Alpha
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