scholarly journals FoxO1a-Cyclic GMP-Dependent Kinase I Interactions Orchestrate Myoblast Fusion

2005 ◽  
Vol 25 (17) ◽  
pp. 7645-7656 ◽  
Author(s):  
Philippe R. J. Bois ◽  
Vanessa F. Brochard ◽  
Adèle V. A. Salin-Cantegrel ◽  
John L. Cleveland ◽  
Gerard C. Grosveld
Keyword(s):  
Cell Differentiation ◽  
Muscle Cell ◽  
Cyclic Gmp ◽  
Molecular Mechanisms ◽  
Binding Activity ◽  
Myoblast Fusion ◽  
Muscle Cell Differentiation ◽  
Dna Binding Activity ◽  
Regulatory Circuits ◽  
Myoblast Cell

ABSTRACT The regulatory circuits that orchestrate mammalian myoblast cell fusion during myogenesis are poorly understood. The transcriptional activity of FoxO1a directly regulates this process, yet the molecular mechanisms governing FoxO1a activity during muscle cell differentiation remain unknown. Here we show an autoregulatory loop in which FoxO1a directly activates transcription of the cyclic GMP-dependent protein kinase I (cGKI) gene and where the ensuing cGKI activity phosphorylates FoxO1a and abolishes its DNA binding activity. These findings establish the FoxO1a-to-cGKI pathway as a novel feedback loop that allows the precise tuning of myoblast fusion. Interestingly, this pathway appears to operate independently of muscle cell differentiation programs directed by myogenic transcription factors.

Cellular and Molecular Mechanisms of Muscle Cell Differentiation in Ascidian Embryos

1990 ◽  
pp. 221-258 ◽  
Author(s):  
Noriyuki Satoh ◽  
Takuya Deno ◽  
Hiroki Nishida ◽  
Takahito Nishikata ◽  
Kazuhiro W. Makabe
Keyword(s):  
Cell Differentiation ◽  
Muscle Cell ◽  
Molecular Mechanisms ◽  
Muscle Cell Differentiation ◽  
Ascidian Embryos

scholarly journals Effect of Ashwagandha Withanolides on Muscle Cell Differentiation

Biomolecules ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1454
Author(s):  
Jia Wang ◽  
Huayue Zhang ◽  
Ashish Kaul ◽  
Kejuan Li ◽  
Didik Priyandoko ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Muscle Cell ◽  
Withania Somnifera ◽  
Molecular Mechanisms ◽  
Disease Model ◽  
Neuromuscular Disorder ◽  
Withaferin A ◽  
C2c12 Myoblasts ◽  
Muscle Cell Differentiation ◽  
Health Promoting

Withania somnifera (Ashwagandha) is used in Indian traditional medicine, Ayurveda, and is believed to have a variety of health-promoting effects. The molecular mechanisms and pathways underlying these effects have not yet been sufficiently explored. In this study, we investigated the effect of Ashwagandha extracts and their major withanolides (withaferin A and withanone) on muscle cell differentiation using C2C12 myoblasts. We found that withaferin A and withanone and Ashwagandha extracts possessing different ratios of these active ingredients have different effects on the differentiation of C2C12. Withanone and withanone-rich extracts caused stronger differentiation of myoblasts to myotubes, deaggregation of heat- and metal-stress-induced aggregated proteins, and activation of hypoxia and autophagy pathways. Of note, the Parkinson’s disease model of Drosophila that possess a neuromuscular disorder showed improvement in their flight and climbing activity, suggesting the potential of Ashwagandha withanolides for the management of muscle repair and activity.

Differential use of SCL/TAL-1 DNA-binding domain in developmental hematopoiesis

Blood ◽  
2008 ◽  
Vol 112 (4) ◽  
pp. 1056-1067 ◽  
Author(s):  
Mira T. Kassouf ◽  
Hedia Chagraoui ◽  
Paresh Vyas ◽  
Catherine Porcher
Keyword(s):  
Dna Binding ◽  
Molecular Mechanisms ◽  
Binding Activity ◽  
Hematopoietic Stem ◽  
Helix Loop Helix ◽  
Experimental Systems ◽  
Dna Binding Activity ◽  
Independent Functions

Abstract Dissecting the molecular mechanisms used by developmental regulators is essential to understand tissue specification/differentiation. SCL/TAL-1 is a basic helix-loop-helix transcription factor absolutely critical for hematopoietic stem/progenitor cell specification and lineage maturation. Using in vitro and forced expression experimental systems, we previously suggested that SCL might have DNA-binding–independent functions. Here, to assess the requirements for SCL DNA-binding activity in vivo, we examined hematopoietic development in mice carrying a germline DNA-binding mutation. Remarkably, in contrast to complete absence of hematopoiesis and early lethality in scl-null embryos, specification of hematopoietic cells occurred in homozygous mutant embryos, indicating that direct DNA binding is dispensable for this process. Lethality was forestalled to later in development, although some mice survived to adulthood. Anemia was documented throughout development and in adulthood. Cellular and molecular studies showed requirements for SCL direct DNA binding in red cell maturation and indicated that scl expression is positively autoregulated in terminally differentiating erythroid cells. Thus, different mechanisms of SCL's action predominate depending on the developmental/cellular context: indirect DNA binding activities and/or sequestration of other nuclear regulators are sufficient in specification processes, whereas direct DNA binding functions with transcriptional autoregulation are critically required in terminal maturation processes.

scholarly journals C6ORF32 is upregulated during muscle cell differentiation and induces the formation of cellular filopodia

2007 ◽  
Vol 301 (1) ◽  
pp. 70-81 ◽  
Author(s):  
Soonsang Yoon ◽  
Michael J. Molloy ◽  
Melissa P. Wu ◽  
Douglas B. Cowan ◽  
Emanuela Gussoni
Keyword(s):  
Cell Differentiation ◽  
Muscle Cell ◽  
Muscle Cell Differentiation
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