scholarly journals A 5'-flanking region of the chicken acetylcholine receptor alpha-subunit gene confers tissue specificity and developmental control of expression in transfected cells.

1987 ◽  
Vol 7 (2) ◽  
pp. 951-955 ◽  
Author(s):  
A Klarsfeld ◽  
P Daubas ◽  
B Bourachot ◽  
J P Changeux
Keyword(s):  
Acetylcholine Receptor ◽  
Chloramphenicol Acetyltransferase ◽  
Alpha Subunit ◽  
Upstream Sequence ◽  
Subunit Gene ◽  
Base Pairs ◽  
Chicken Muscle ◽  
Flanking Region ◽  
Chloramphenicol Acetyltransferase Gene ◽  
Control Of Expression

The 5' end and promoter region of the alpha-subunit gene of chicken muscle acetylcholine receptor was mapped and sequenced. It includes a TATA and a CAAT box and a potential Sp1-binding site. When inserted in front of the chloramphenicol acetyltransferase gene, this promoter (including 850 base pairs of upstream sequence) directed high transient chloramphenicol acetyltransferase expression in transfected mouse C2.7 myotubes but not in C2.7 myoblasts or nonmyogenic 3T6 cells.

A 5'-flanking region of the chicken acetylcholine receptor alpha-subunit gene confers tissue specificity and developmental control of expression in transfected cells

1987 ◽  
Vol 7 (2) ◽  
pp. 951-955
Author(s):  
A Klarsfeld ◽  
P Daubas ◽  
B Bourachot ◽  
J P Changeux
Keyword(s):  
Acetylcholine Receptor ◽  
Chloramphenicol Acetyltransferase ◽  
Alpha Subunit ◽  
Upstream Sequence ◽  
Subunit Gene ◽  
Base Pairs ◽  
Chicken Muscle ◽  
Flanking Region ◽  
Chloramphenicol Acetyltransferase Gene ◽  
Control Of Expression

The 5' end and promoter region of the alpha-subunit gene of chicken muscle acetylcholine receptor was mapped and sequenced. It includes a TATA and a CAAT box and a potential Sp1-binding site. When inserted in front of the chloramphenicol acetyltransferase gene, this promoter (including 850 base pairs of upstream sequence) directed high transient chloramphenicol acetyltransferase expression in transfected mouse C2.7 myotubes but not in C2.7 myoblasts or nonmyogenic 3T6 cells.

scholarly journals Localization of nicotinic acetylcholine receptor alpha-subunit transcripts during myogenesis and motor endplate development in the chick.

1989 ◽  
Vol 108 (3) ◽  
pp. 1025-1037 ◽  
Author(s):  
B Fontaine ◽  
J P Changeux
Keyword(s):  
Acetylcholine Receptor ◽  
Nicotinic Acetylcholine Receptor ◽  
Muscle Development ◽  
Neuromuscular Junctions ◽  
Primary Cultures ◽  
Motor Endplate ◽  
Alpha Subunit ◽  
Subunit Gene ◽  
Nicotinic Acetylcholine

In 15-d-old chick latissimi dorsi muscles, the nicotinic acetylcholine receptor (AChR) alpha-subunit mRNA is densely accumulated at the level of subsynaptic nuclei of the motor endplate (Fontaine et al., 1988). In this paper, using in situ hybridization with genomic probes, we further show that the expression of the AChR alpha-subunit gene in the embryo, revealed by the accumulation of mature mRNAs, starts in myotomal cells and persists during the first stages of muscle development in a majority of muscle nuclei. Subsequently, the distribution of AChR alpha-subunit mRNAs becomes restricted to the newly formed motor endplates as neuromuscular junctions develop. To assess the transcriptional activity of individual nuclei in developing muscles, a strictly intronic fragment of the AChR alpha-subunit gene was used to probe in situ the level of unspliced transcripts. AChR alpha-subunit unspliced transcripts accumulate around a large number of sarcoplasmic nuclei at embryonic day 11, but can no longer be detected at their level after embryonic day 16 in the embryo. A similar decrease in the accumulation of AChR alpha-subunit transcripts is observed between day 4 and day 6 in primary cultures of muscle cells. On the other hand, in vivo denervation and in vitro blocking of muscle electrical activity by the sodium channel blocker tetrodotoxin results in an increase in the labeling of muscle nuclei. Yet, only 6% of the muscle nuclei appear labeled by the strictly intronic probes after denervation. The possible significance of such heterogeneity of muscle nuclei during motor endplate formation in AChR gene expression is discussed.

Transcription of the procyclic acidic repetitive protein genes of Trypanosoma brucei

1990 ◽  
Vol 10 (6) ◽  
pp. 3036-3047
Author(s):  
C E Clayton ◽  
J P Fueri ◽  
J E Itzhaki ◽  
V Bellofatto ◽  
D R Sherman ◽  
...  
Keyword(s):  
Trypanosoma Brucei ◽  
Chloramphenicol Acetyltransferase ◽  
Surface Proteins ◽  
Base Pairs ◽  
Developmentally Regulated ◽  
Putative Promoter Region ◽  
Transcriptional Initiation ◽  
Chloramphenicol Acetyltransferase Gene ◽  
Alpha Amanitin ◽  
Repetitive Protein

The procyclic acidic repetitive protein (parp) genes of Trypanosoma brucei encode a small family of abundant surface proteins whose expression is restricted to the procyclic form of the parasite. They are found at two unlinked loci, parpA and parpB; transcription of both loci is developmentally regulated. The region of homology upstream of the A and B parp genes is only 640 base pairs long and may contain sequences responsible for transcriptional initiation and regulation. Transcription upstream of this putative promoter region is not developmentally regulated and is much less active than that of the parp genes; the polymerase responsible is inhibited by alpha-amanitin, whereas that transcribing the parp genes is not. Transcription of the parp genes is strongly stimulated by low levels of UV irradiation. The putative parp promoter, when placed upstream of the chloramphenicol acetyltransferase gene, is sufficient to cause production of chloramphenicol acetyltransferase in a T. brucei DNA transformation assay. Taken together, these results suggest that a promoter for an alpha-amanitin-resistant RNA polymerase lies less than 600 nucleotides upstream of the parp genes.

Human Muscle Acetylcholine Receptor Alpha-subunit Gene (CHRNA1) Association with Autoimmune Myasthenia Gravis in Black, Mixed-Ancestry and Caucasian Subjects

1996 ◽  
Vol 9 (2) ◽  
pp. 175-180 ◽  
Author(s):  
Jeannine M. Heckmann ◽  
Karen E. Morrison ◽  
Barbara Emeryk-Szajewska ◽  
Halina Strugalska ◽  
JoAnn Bergoffen ◽  
...  
Keyword(s):  
Myasthenia Gravis ◽  
Acetylcholine Receptor ◽  
Human Muscle ◽  
Alpha Subunit ◽  
Subunit Gene ◽  
Mixed Ancestry ◽  
Receptor Alpha ◽  
Autoimmune Myasthenia

scholarly journals Involvement of human muscle acetylcholine receptor alpha-subunit gene (CHRNA) in susceptibility to myasthenia gravis.

1994 ◽  
Vol 91 (11) ◽  
pp. 4668-4672 ◽  
Author(s):  
H. J. Garchon ◽  
F. Djabiri ◽  
J. P. Viard ◽  
P. Gajdos ◽  
J. F. Bach
Keyword(s):  
Myasthenia Gravis ◽  
Acetylcholine Receptor ◽  
Human Muscle ◽  
Alpha Subunit ◽  
Subunit Gene ◽  
Receptor Alpha
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