scholarly journals Hormonal regulation of TSE1-repressed genes: evidence for multiple genetic controls in extinction.

1989 ◽  
Vol 9 (7) ◽  
pp. 2837-2846 ◽  
Author(s):  
M J Thayer ◽  
R E Fournier
Keyword(s):  
Hormonal Regulation ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Genetic Locus ◽  
Tyrosine Aminotransferase ◽  
Chromosome 17 ◽  
Chromosome 11 ◽  
Liver Functions ◽  
Genes Encoding ◽  
Human Chromosome 17 ◽  
Mouse Chromosome 11

Somatic cell hybrids formed by fusing hepatoma cells with fibroblasts generally fail to express liver functions, a phenomenon termed extinction. Previous studies demonstrated that extinction of the genes encoding tyrosine aminotransferase, phosphoenolpyruvate carboxykinase, and argininosuccinate synthetase is mediated by a specific genetic locus (TSE1) that maps to mouse chromosome 11 and human chromosome 17. In this report, we show that full repression of these genes requires a genetic factor in addition to TSE1. This conclusion is based on the observation that residual gene activity was apparent in monochromosomal hybrids retaining human TSE1 but not in complex hybrids retaining many fibroblast chromosomes. Furthermore, TSE1-repressed genes were hormone inducible, whereas fully extinguished genes were not. Analysis of hybrid segregants indicated that genetic loci required for the complete repression phenotype were distinct from TSE1.

Hormonal regulation of TSE1-repressed genes: evidence for multiple genetic controls in extinction

1989 ◽  
Vol 9 (7) ◽  
pp. 2837-2846
Author(s):  
M J Thayer ◽  
R E Fournier
Keyword(s):  
Hormonal Regulation ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Genetic Locus ◽  
Tyrosine Aminotransferase ◽  
Chromosome 17 ◽  
Chromosome 11 ◽  
Liver Functions ◽  
Genes Encoding ◽  
Human Chromosome 17 ◽  
Mouse Chromosome 11

Somatic cell hybrids formed by fusing hepatoma cells with fibroblasts generally fail to express liver functions, a phenomenon termed extinction. Previous studies demonstrated that extinction of the genes encoding tyrosine aminotransferase, phosphoenolpyruvate carboxykinase, and argininosuccinate synthetase is mediated by a specific genetic locus (TSE1) that maps to mouse chromosome 11 and human chromosome 17. In this report, we show that full repression of these genes requires a genetic factor in addition to TSE1. This conclusion is based on the observation that residual gene activity was apparent in monochromosomal hybrids retaining human TSE1 but not in complex hybrids retaining many fibroblast chromosomes. Furthermore, TSE1-repressed genes were hormone inducible, whereas fully extinguished genes were not. Analysis of hybrid segregants indicated that genetic loci required for the complete repression phenotype were distinct from TSE1.

Differential activity of a tissue-specific extinguisher locus in hepatic and nonhepatic cells

1989 ◽  
Vol 9 (5) ◽  
pp. 1813-1822
Author(s):  
H Gourdeau ◽  
T C Peterson ◽  
R E Fournier
Keyword(s):  
Mouse Chromosome ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Genetic Locus ◽  
Cell Types ◽  
Tyrosine Aminotransferase ◽  
Hepatoma Cells ◽  
Chromosome 11 ◽  
Tissue Specific ◽  
Primary Mouse ◽  
Mouse Chromosome 11

Tissue-specific extinguisher 1 (Tse-1) is a genetic locus on mouse chromosome 11 that can repress expression of several liver genes in trans. This locus is clearly active in fibroblasts, as hepatoma cells retaining fibroblast chromosome 11 are extinguished for both tyrosine aminotransferase and phosphoenolpyruvate carboxykinase gene expression. To assess the activity of Tse-1 in other tissues, we transferred mouse chromosome 11 from several different cell types into rat hepatoma recipients. Tse-1 was active in nonhepatic cell lines derived from each primary germ layer, but Tse-1 activity was not apparent in hybrids between hepatoma cells and primary mouse hepatocytes. These differences in the genetic activity of murine Tse-1 were apparently heritable in cis.

scholarly journals Differential activity of a tissue-specific extinguisher locus in hepatic and nonhepatic cells.

1989 ◽  
Vol 9 (5) ◽  
pp. 1813-1822 ◽  
Author(s):  
H Gourdeau ◽  
T C Peterson ◽  
R E Fournier
Keyword(s):  
Mouse Chromosome ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Genetic Locus ◽  
Cell Types ◽  
Tyrosine Aminotransferase ◽  
Hepatoma Cells ◽  
Chromosome 11 ◽  
Tissue Specific ◽  
Primary Mouse ◽  
Mouse Chromosome 11

Tissue-specific extinguisher 1 (Tse-1) is a genetic locus on mouse chromosome 11 that can repress expression of several liver genes in trans. This locus is clearly active in fibroblasts, as hepatoma cells retaining fibroblast chromosome 11 are extinguished for both tyrosine aminotransferase and phosphoenolpyruvate carboxykinase gene expression. To assess the activity of Tse-1 in other tissues, we transferred mouse chromosome 11 from several different cell types into rat hepatoma recipients. Tse-1 was active in nonhepatic cell lines derived from each primary germ layer, but Tse-1 activity was not apparent in hybrids between hepatoma cells and primary mouse hepatocytes. These differences in the genetic activity of murine Tse-1 were apparently heritable in cis.

The Gene Encoding Protein Kinase SEK1 Maps to Mouse Chromosome 11 and Human Chromosome 17

Genomics ◽  
1996 ◽  
Vol 34 (3) ◽  
pp. 430-432 ◽  
Author(s):  
Robert A. White ◽  
Rowland T. Hughes ◽  
Linda R. Adkison ◽  
Gail Bruns ◽  
Leonard I. Zon
Keyword(s):  
Protein Kinase ◽  
Human Chromosome ◽  
Mouse Chromosome ◽  
Chromosome 17 ◽  
Chromosome 11 ◽  
Gene Encoding ◽  
Human Chromosome 17 ◽  
Mouse Chromosome 11 ◽  
Encoding Protein

Assignment ofAmog (adhesion molecule on glia) gene to mouse chromosome 11 nearZfp-3 andAsgr-1,2 and to human chromosome 17

1990 ◽  
Vol 16 (4) ◽  
pp. 401-405 ◽  
Author(s):  
Chih-Lin Hsieh ◽  
Andrea Cheng-Deutsch ◽  
Sergio Gloor ◽  
Melitta Schachner ◽  
Uta Francke
Keyword(s):  
Human Chromosome ◽  
Mouse Chromosome ◽  
Adhesion Molecule ◽  
Chromosome 17 ◽  
Chromosome 11 ◽  
Human Chromosome 17 ◽  
Mouse Chromosome 11

scholarly journals A comprehensive genetic map of murine chromosome 11 reveals extensive linkage conservation between mouse and human.

Genetics ◽  
1989 ◽  
Vol 122 (1) ◽  
pp. 153-161 ◽  
Author(s):  
A M Buchberg ◽  
E Brownell ◽  
S Nagata ◽  
N A Jenkins ◽  
N G Copeland
Keyword(s):  
Human Chromosome ◽  
Linkage Map ◽  
Mouse Chromosome ◽  
Chromosome 17 ◽  
Human Chromosomes ◽  
Chromosome 11 ◽  
Murine Chromosome ◽  
Human Chromosome 17 ◽  
Mouse Chromosome 11 ◽  
Linkage Conservation

Abstract Interspecific backcross animals from a cross between C57BL/6J and Mus spretus mice were used to generate a comprehensive linkage map of mouse chromosome 11. The relative map positions of genes previously assigned to mouse chromosome 11 by somatic cell hybrid or genetic backcross analysis were determined (Erbb, Rel, 11-3, Csfgm, Trp53-1, Evi-2, Erba, Erbb-2, Csfg, Myhs, Cola-1, Myla, Hox-2 and Pkca). We also analyzed genes that we suspected would map to chromosome 11 by virtue of their location in human chromosomes and the known linkage homologies that exist between murine chromosome 11 and human chromosomes (Mpo, Ngfr, Pdgfr and Fms). Two of the latter genes, Mpo and Ngfr, mapped to mouse chromosome 11. Both genes also mapped to human chromosome 17, extending the degree of linkage conservation observed between human chromosome 17 and mouse chromosome 11. Pdgfr and Fms, which are closely linked to II-3 and Csfgm in humans on chromosome 5, mapped to mouse chromosome 18 rather than mouse chromosome 11, thereby defining yet another conserved linkage group between human and mouse chromosomes. The mouse chromosome 11 linkage map generated in these studies substantially extends the framework for identifying homologous genes in the mouse that are involved in human disease, for elucidating the genes responsible for several mouse mutations, and for gaining insights into chromosome evolution and genome organization.

Regulation of chimeric phosphoenolpyruvate carboxykinase genes by the trans-dominant locus TSE1

1990 ◽  
Vol 10 (6) ◽  
pp. 2660-2668
Author(s):  
M J Thayer ◽  
T G Lugo ◽  
R J Leach ◽  
R E Fournier
Keyword(s):  
Gene Expression ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Genetic Locus ◽  
Chromosome 17 ◽  
Gene Sequences ◽  
Base Pairs ◽  
Tissue Specific ◽  
Chimeric Genes ◽  
Microcell Hybrids ◽  
Human Chromosome 17

Extinction of phosphoenolpyruvate carboxykinase (PCK) gene expression in hepatoma x fibroblast hybrids is mediated by a trans-acting genetic locus designated tissue-specific extinguisher 1 (TSE1). To identify PCK gene sequences required for extinction, hepatoma transfectants expressing PCK-thymidine kinase (TK) chimeric genes were fused with TK- fibroblasts and PCK-TK expression in the resulting hybrids was monitored. Expression of a PCK-TK chimera containing PCK sequences between base pairs -548 and +73 was extinguished in four of five hepatoma transfectants tested, although hybrids derived from one transfectant clone failed to extinguish PCK-TK expression. In contrast, crosses between hepatoma transfectants expressing the herpesvirus TK gene from its own promoter and TK- fibroblasts produced TK+ hybrids; extinction of the transfected TK gene was not observed. Thus, rat PCK gene sequences between base pairs -548 and +73 are sufficient for tissue-specific extinction in hybrid cells. Extinction of PCK-TK gene expression in transfectant microcell hybrids mapped specifically to human chromosome 17, the site of human TSE1.

Genetic map of nine polymorphic loci comprising a single linkage group on rat chromosome 10: Evidence for linkage conservation with human chromosome 17 and mouse chromosome 11

Genomics ◽  
1992 ◽  
Vol 14 (3) ◽  
pp. 618-623 ◽  
Author(s):  
Elaine F. Remmers ◽  
Ellen A. Goldmuntz ◽  
Joseph M. Cash ◽  
Leslie J. Crofford ◽  
Barbara Misiewicz-Poltorak ◽  
...  
Keyword(s):  
Linkage Group ◽  
Human Chromosome ◽  
Mouse Chromosome ◽  
Genetic Map ◽  
Chromosome 17 ◽  
Chromosome 11 ◽  
Single Linkage ◽  
Human Chromosome 17 ◽  
Mouse Chromosome 11 ◽  
Linkage Conservation

scholarly journals Regulation of chimeric phosphoenolpyruvate carboxykinase genes by the trans-dominant locus TSE1.

1990 ◽  
Vol 10 (6) ◽  
pp. 2660-2668 ◽  
Author(s):  
M J Thayer ◽  
T G Lugo ◽  
R J Leach ◽  
R E Fournier
Keyword(s):  
Gene Expression ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Genetic Locus ◽  
Chromosome 17 ◽  
Gene Sequences ◽  
Base Pairs ◽  
Tissue Specific ◽  
Chimeric Genes ◽  
Microcell Hybrids ◽  
Human Chromosome 17

Extinction of phosphoenolpyruvate carboxykinase (PCK) gene expression in hepatoma x fibroblast hybrids is mediated by a trans-acting genetic locus designated tissue-specific extinguisher 1 (TSE1). To identify PCK gene sequences required for extinction, hepatoma transfectants expressing PCK-thymidine kinase (TK) chimeric genes were fused with TK- fibroblasts and PCK-TK expression in the resulting hybrids was monitored. Expression of a PCK-TK chimera containing PCK sequences between base pairs -548 and +73 was extinguished in four of five hepatoma transfectants tested, although hybrids derived from one transfectant clone failed to extinguish PCK-TK expression. In contrast, crosses between hepatoma transfectants expressing the herpesvirus TK gene from its own promoter and TK- fibroblasts produced TK+ hybrids; extinction of the transfected TK gene was not observed. Thus, rat PCK gene sequences between base pairs -548 and +73 are sufficient for tissue-specific extinction in hybrid cells. Extinction of PCK-TK gene expression in transfectant microcell hybrids mapped specifically to human chromosome 17, the site of human TSE1.

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