Differential activity of a tissue-specific extinguisher locus in hepatic and nonhepatic cells

1989 ◽  
Vol 9 (5) ◽  
pp. 1813-1822
Author(s):  
H Gourdeau ◽  
T C Peterson ◽  
R E Fournier
Keyword(s):  
Mouse Chromosome ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Genetic Locus ◽  
Cell Types ◽  
Tyrosine Aminotransferase ◽  
Hepatoma Cells ◽  
Chromosome 11 ◽  
Tissue Specific ◽  
Primary Mouse ◽  
Mouse Chromosome 11

Tissue-specific extinguisher 1 (Tse-1) is a genetic locus on mouse chromosome 11 that can repress expression of several liver genes in trans. This locus is clearly active in fibroblasts, as hepatoma cells retaining fibroblast chromosome 11 are extinguished for both tyrosine aminotransferase and phosphoenolpyruvate carboxykinase gene expression. To assess the activity of Tse-1 in other tissues, we transferred mouse chromosome 11 from several different cell types into rat hepatoma recipients. Tse-1 was active in nonhepatic cell lines derived from each primary germ layer, but Tse-1 activity was not apparent in hybrids between hepatoma cells and primary mouse hepatocytes. These differences in the genetic activity of murine Tse-1 were apparently heritable in cis.

scholarly journals Differential activity of a tissue-specific extinguisher locus in hepatic and nonhepatic cells.

1989 ◽  
Vol 9 (5) ◽  
pp. 1813-1822 ◽  
Author(s):  
H Gourdeau ◽  
T C Peterson ◽  
R E Fournier
Keyword(s):  
Mouse Chromosome ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Genetic Locus ◽  
Cell Types ◽  
Tyrosine Aminotransferase ◽  
Hepatoma Cells ◽  
Chromosome 11 ◽  
Tissue Specific ◽  
Primary Mouse ◽  
Mouse Chromosome 11

Tissue-specific extinguisher 1 (Tse-1) is a genetic locus on mouse chromosome 11 that can repress expression of several liver genes in trans. This locus is clearly active in fibroblasts, as hepatoma cells retaining fibroblast chromosome 11 are extinguished for both tyrosine aminotransferase and phosphoenolpyruvate carboxykinase gene expression. To assess the activity of Tse-1 in other tissues, we transferred mouse chromosome 11 from several different cell types into rat hepatoma recipients. Tse-1 was active in nonhepatic cell lines derived from each primary germ layer, but Tse-1 activity was not apparent in hybrids between hepatoma cells and primary mouse hepatocytes. These differences in the genetic activity of murine Tse-1 were apparently heritable in cis.

scholarly journals Hormonal regulation of TSE1-repressed genes: evidence for multiple genetic controls in extinction.

1989 ◽  
Vol 9 (7) ◽  
pp. 2837-2846 ◽  
Author(s):  
M J Thayer ◽  
R E Fournier
Keyword(s):  
Hormonal Regulation ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Genetic Locus ◽  
Tyrosine Aminotransferase ◽  
Chromosome 17 ◽  
Chromosome 11 ◽  
Liver Functions ◽  
Genes Encoding ◽  
Human Chromosome 17 ◽  
Mouse Chromosome 11

Somatic cell hybrids formed by fusing hepatoma cells with fibroblasts generally fail to express liver functions, a phenomenon termed extinction. Previous studies demonstrated that extinction of the genes encoding tyrosine aminotransferase, phosphoenolpyruvate carboxykinase, and argininosuccinate synthetase is mediated by a specific genetic locus (TSE1) that maps to mouse chromosome 11 and human chromosome 17. In this report, we show that full repression of these genes requires a genetic factor in addition to TSE1. This conclusion is based on the observation that residual gene activity was apparent in monochromosomal hybrids retaining human TSE1 but not in complex hybrids retaining many fibroblast chromosomes. Furthermore, TSE1-repressed genes were hormone inducible, whereas fully extinguished genes were not. Analysis of hybrid segregants indicated that genetic loci required for the complete repression phenotype were distinct from TSE1.

Hormonal regulation of TSE1-repressed genes: evidence for multiple genetic controls in extinction

1989 ◽  
Vol 9 (7) ◽  
pp. 2837-2846
Author(s):  
M J Thayer ◽  
R E Fournier
Keyword(s):  
Hormonal Regulation ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Genetic Locus ◽  
Tyrosine Aminotransferase ◽  
Chromosome 17 ◽  
Chromosome 11 ◽  
Liver Functions ◽  
Genes Encoding ◽  
Human Chromosome 17 ◽  
Mouse Chromosome 11

Somatic cell hybrids formed by fusing hepatoma cells with fibroblasts generally fail to express liver functions, a phenomenon termed extinction. Previous studies demonstrated that extinction of the genes encoding tyrosine aminotransferase, phosphoenolpyruvate carboxykinase, and argininosuccinate synthetase is mediated by a specific genetic locus (TSE1) that maps to mouse chromosome 11 and human chromosome 17. In this report, we show that full repression of these genes requires a genetic factor in addition to TSE1. This conclusion is based on the observation that residual gene activity was apparent in monochromosomal hybrids retaining human TSE1 but not in complex hybrids retaining many fibroblast chromosomes. Furthermore, TSE1-repressed genes were hormone inducible, whereas fully extinguished genes were not. Analysis of hybrid segregants indicated that genetic loci required for the complete repression phenotype were distinct from TSE1.

scholarly journals XChromosome Effect on Maternal Recombination and Meiotic Drive in the Mouse

Genetics ◽  
2002 ◽  
Vol 161 (4) ◽  
pp. 1651-1659 ◽  
Author(s):  
Elena de la Casa-Esperón ◽  
J Concepción Loredo-Osti ◽  
Fernando Pardo-Manuel de Villena ◽  
Tammi L Briscoe ◽  
Jan Michel Malette ◽  
...  
Keyword(s):  
Mouse Chromosome ◽  
X Chromosome ◽  
Meiotic Recombination ◽  
Meiotic Drive ◽  
X Chromosome Inactivation ◽  
Chromosome Inactivation ◽  
Chromosome 11 ◽  
Genome Wide ◽  
Mouse Chromosome 11 ◽  
Segregation Of Chromosomes

AbstractWe observed that maternal meiotic drive favoring the inheritance of DDK alleles at the Om locus on mouse chromosome 11 was correlated with the X chromosome inactivation phenotype of (C57BL/ 6-Pgk1a × DDK)F1 mothers. The basis for this unexpected observation appears to lie in the well-documented effect of recombination on meiotic drive that results from nonrandom segregation of chromosomes. Our analysis of genome-wide levels of meiotic recombination in females that vary in their X-inactivation phenotype indicates that an allelic difference at an X-linked locus is responsible for modulating levels of recombination in oocytes.

New testis-specific expressed genes on mouse Chromosome 11

1996 ◽  
Vol 7 (2) ◽  
pp. 163-163
Author(s):  
G. C. Voss ◽  
H. Jockusch
Keyword(s):  
Mouse Chromosome ◽  
Chromosome 11 ◽  
Mouse Chromosome 11

The Gene Encoding Protein Kinase SEK1 Maps to Mouse Chromosome 11 and Human Chromosome 17

Genomics ◽  
1996 ◽  
Vol 34 (3) ◽  
pp. 430-432 ◽  
Author(s):  
Robert A. White ◽  
Rowland T. Hughes ◽  
Linda R. Adkison ◽  
Gail Bruns ◽  
Leonard I. Zon
Keyword(s):  
Protein Kinase ◽  
Human Chromosome ◽  
Mouse Chromosome ◽  
Chromosome 17 ◽  
Chromosome 11 ◽  
Gene Encoding ◽  
Human Chromosome 17 ◽  
Mouse Chromosome 11 ◽  
Encoding Protein
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