scholarly journals Complete Genome Sequence of Human Oral Actinomyces sp. HMT897 Strain ORNL0104, a Host of the Saccharibacterium (TM7) HMT351

2021 ◽  
Vol 10 (14) ◽  
Author(s):  
Karissa L. Cross ◽  
Floyd Dewhirst ◽  
Mircea Podar

ABSTRACT Actinomyces sp. HMT897 strain ORNL0104 was isolated from a human saliva sample and can serve as a host for the ectobiont Saccharibacteria (TM7) HMT351. Its 3.3-Mb chromosome was completely sequenced using PacBio long reads, and it encodes 2,407 proteins and 63 RNAs.

2021 ◽  
Vol 10 (23) ◽  
Author(s):  
Snehal Joshi ◽  
Peter T. Podar ◽  
Floyd E. Dewhirst ◽  
Mircea Podar

Actinomyces sp. HMT175 strain ORNL0102 was isolated from a human saliva sample and can serve as a host for the ectobiont saccharibacterium/TM7 HMT957. Its 3.3-Mbp circular chromosome was completely sequenced using PacBio long reads, and it encodes 2,408 proteins and 63 RNAs.


2017 ◽  
Vol 5 (22) ◽  
Author(s):  
Peng Zhou ◽  
Gary Xie ◽  
Xiaoli Li ◽  
Jinman Liu ◽  
Fengxia Qi

ABSTRACT The Veillonella atypica strain OK5 was isolated from a human saliva sample and was the first strain shown to be genetically transformable in the Veillonella genus. Genetic studies using this strain have helped us gain much insight into the ecology of human oral biofilms. Here, we report the complete genome sequence of V. atypica OK5.


2018 ◽  
Vol 6 (18) ◽  
pp. e00230-18 ◽  
Author(s):  
Vineeth Kodengil Vettath ◽  
Ana Carolina M. Junqueira ◽  
Akira Uchida ◽  
Rikky W. Purbojati ◽  
James N. I. Houghton ◽  
...  

ABSTRACT Acinetobacter indicus (Gammaproteobacteria) is a strict aerobic nonmotile bacterium. The strain SGAir0564 was isolated from air samples collected in Singapore. The complete genome is 3.1 Mb and was assembled using a combination of short and long reads. The genome contains 2,808 protein-coding genes, 80 tRNAs, and 21 rRNA subunits.


2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Suganniiya K. Ravintheran ◽  
Sumitra Sivaprakasam ◽  
Stella Loke ◽  
Su Yin Lee ◽  
Ravichandran Manickam ◽  
...  

AbstractComplete genomes of xenobiotic-degrading microorganisms provide valuable resources for researchers to understand molecular mechanisms involved in bioremediation. Despite the well-known ability of Sphingomonas paucimobilis to degrade persistent xenobiotic compounds, a complete genome sequencing is lacking for this organism. In line with this, we report the first complete genome sequence of Sphingomonas paucimobilis (strain AIMST S2), an organophosphate and hydrocarbon-degrading bacterium isolated from oil-polluted soil at Kedah, Malaysia. The genome was derived from a hybrid assembly of short and long reads generated by Illumina HiSeq and MinION, respectively. The assembly resulted in a single contig of 4,005,505 bases which consisted of 3,612 CDS and 56 tRNAs. An array of genes involved in xenobiotic degradation and plant-growth promoters were identified, suggesting its’ potential role as an effective microorganism in bioremediation and agriculture. Having reported the first complete genome of the species, this study will serve as a stepping stone for comparative genome analysis of Sphingomonas strains and other xenobiotic-degrading microorganisms as well as gene expression studies in organophosphate biodegradation.


2019 ◽  
Vol 8 (47) ◽  
Author(s):  
Phu Pwint Thin Hlaing ◽  
Ana Carolina M. Junqueira ◽  
Akira Uchida ◽  
Rikky W. Purbojati ◽  
Anthony Wong ◽  
...  

Curtobacterium sp. strain SGAir0471 was isolated from tropical air samples collected in Singapore. The genome was assembled using PacBio RS II long reads and Illumina MiSeq short paired-end reads. The complete genome measures 3.53 Mb and consists of 3,151 protein-coding genes, 49 tRNAs, and 12 rRNAs.


2021 ◽  
Vol 10 (42) ◽  
Author(s):  
Yoshi Yamano ◽  
Sachiko Sugimoto ◽  
Katsuyoshi Matsunami

Here, we described the closed complete genome sequence of Actinoplanes sp. strain L3-i22, which was obtained from the assembly with long reads and subsequent polishing with short reads. The complete genome consists of a 12,014,766-bp chromosome, with a GC content of 71.4%, and contains no plasmids.


2020 ◽  
Vol 9 (47) ◽  
Author(s):  
Nardjess Chéraiti ◽  
Frédéric Plewniak ◽  
Salima Tighidet ◽  
Amalia Sayeh ◽  
Lisa Gil ◽  
...  

ABSTRACT Microbacterium sp. strain Nx66 was isolated from waters contaminated by petrochemical effluents collected in Algeria. Its genome was sequenced using Illumina MiSeq (2 × 150-bp read pairs) and Oxford Nanopore (long reads) technologies and was assembled using Unicycler. It is composed of one chromosome of 3.42 Mb and one plasmid of 34.22 kb.


2020 ◽  
Vol 9 (27) ◽  
Author(s):  
Mircea Podar ◽  
Joel Turner ◽  
Leah H. Burdick ◽  
Dale A. Pelletier

ABSTRACT Roseimicrobium sp. strain ORNL1 is a soil bacterium that belongs to the phylum Verrucomicrobia and was isolated from the rhizosphere of a forest Eastern cottonwood tree, Populus deltoides, in Tennessee. Its 7.9-Mb chromosome was completely sequenced using PacBio long reads and is predicted to encode 6,288 proteins and 76 RNAs.


2019 ◽  
Vol 8 (32) ◽  
Author(s):  
Phu Pwint Thin Hlaing ◽  
Ana Carolina M. Junqueira ◽  
Akira Uchida ◽  
Rikky W. Purbojati ◽  
James N. I. Houghton ◽  
...  

Brachybacterium sp. strain SGAir0954 was isolated from tropical air collected in Singapore, and its genome was sequenced and assembled using long reads generated by single-molecule real-time (SMRT) sequencing. The complete genome has a size of 3.41 Mb and consists of 2,955 protein coding genes, 50 tRNAs, and 9 rRNAs.


2018 ◽  
Vol 6 (18) ◽  
Author(s):  
Cassie E. Heinle ◽  
Ana Carolina M. Junqueira ◽  
Akira Uchida ◽  
Rikky W. Purbojati ◽  
James N. I. Houghton ◽  
...  

ABSTRACT Lelliottia nimipressuralis type strain SGAir0187 was isolated from tropical air samples collected in Singapore. The genome was assembled with an average coverage of 180-fold using Pacific Biosciences long reads and Illumina MiSeq paired-end reads. The genome measures 4.8 Mb and contains 4,424 protein-coding genes, 83 tRNAs, and 25 rRNAs.


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