scholarly journals Complete Genome Sequence of Pandoraea pnomenusa TF-18, a Multidrug-Resistant Organism Isolated from the Rhizosphere of Rice (Oryza sativa L. subsp. japonica)

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Mihnea R. Mangalea ◽  
Emily K. Luna ◽  
Janet Ziegle ◽  
Christine Chang ◽  
Angela M. Bosco-Lauth ◽  
...  
Keyword(s):  
Single Molecule ◽  
Complete Genome ◽  
Oryza Sativa L ◽  
Gc Content ◽  
Multidrug Resistant ◽  
Selective Medium ◽  
Resistant Organism ◽  
Smrt Sequencing ◽  
Sequencing Technology ◽  
Content Type

Pandoraea pnomenusa strain TF-18 was isolated from the roots of rice seedlings on selective medium containing four classes of antibiotics for isolation of Burkholderia pseudomallei. Using Pacific Biosciences (PacBio) single-molecule real-time (SMRT) sequencing technology, we report here a complete genome of 5,499,432 bases, a GC content of 64.8%, and 4,849 coding sequences.

scholarly journals Complete Genome Sequence of Acidithiobacillus ferridurans JAGS, Isolated from Acidic Mine Drainage

2020 ◽  
Vol 9 (13) ◽  
Author(s):  
Jinjin Chen ◽  
Yilan Liu ◽  
Patrick Diep ◽  
Andrew Jo ◽  
Camilla Nesbø ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Single Molecule ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Mine Drainage ◽  
Gc Content ◽  
Acidic Mine Drainage ◽  
Smrt Sequencing ◽  
Protein Coding ◽  
Content Type

We report a complete genome sequence of Acidithiobacillus ferridurans JAGS, determined using PacBio single-molecule real-time (SMRT) sequencing. The circular genome of JAGS (2,933,811 bp; GC content, 58.57%) contains 3,001 protein-coding sequences, 46 tRNAs, and 6 rRNAs. Predicted genes indicate the potential to fix CO2 and N2 and to utilize Fe2+, S0, and H2 as energy sources.

scholarly journals Four Complete Paenibacillus larvae Genome Sequences

2017 ◽  
Vol 5 (22) ◽  
Author(s):  
Douglas W. Dingman
Keyword(s):  
Single Molecule ◽  
Complete Genome ◽  
Size Range ◽  
Molecular Size ◽  
Paenibacillus Larvae ◽  
Smrt Sequencing ◽  
Sequencing Technology ◽  
Genome Sequences ◽  
Sequence Determination ◽  
Content Type

ABSTRACT Four complete genome sequences of genetically distinct Paenibacillus larvae strains have been determined. Pacific BioSciences single-molecule real-time (SMRT) sequencing technology was used as the sole method of sequence determination and assembly. The chromosomes exhibited a G+C content of 44.1 to 44.2% and a molecular size range of 4.29 to 4.67 Mbp.

scholarly journals Complete Genome Sequence and Methylome Analysis of Acinetobacter calcoaceticus 65

2017 ◽  
Vol 5 (12) ◽  
Author(s):  
Alexey Fomenkov ◽  
Tamas Vincze ◽  
Sergey K. Degtyarev ◽  
Richard J. Roberts
Keyword(s):  
Single Molecule ◽  
Restriction Enzyme ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Acinetobacter Calcoaceticus ◽  
Complete Sequence ◽  
Smrt Sequencing ◽  
Original Source ◽  
Content Type ◽  
Methylome Analysis

ABSTRACT Acinetobacter calcoaceticus 65 is the original source strain for the restriction enzyme Acc65I. Its complete sequence and full methylome were determined using single-molecule real-time (SMRT) sequencing.

scholarly journals Complete Genome and Methylome Analysis of the Box-Shaped Halophilic Archaeon Haloarcula sinaiiensis ATCC 33800

2021 ◽  
Vol 10 (34) ◽  
Author(s):  
Alexey Fomenkov ◽  
Priya DasSarma ◽  
Sean P. Kennedy ◽  
Richard J. Roberts ◽  
Shiladitya DasSarma
Keyword(s):  
Real Time ◽  
Single Molecule ◽  
Complete Genome ◽  
Bioinformatic Analysis ◽  
Halophilic Archaeon ◽  
Smrt Sequencing ◽  
Content Type ◽  
Methylome Analysis

The genome of halophilic archaeon Haloarcula sinaiiensis ATCC 33800 was sequenced and assembled and comprises seven replicons. Four m6A and one m4C modified motifs and their responsible methyltransferase genes have been identified in the genome by single-molecule real-time (SMRT) sequencing and bioinformatic analysis.

scholarly journals Complete Genome Sequence of Lactobacillus fermentum MTCC 25067 (Formerly TDS030603), a Viscous Exopolysaccharide-Producing Strain Isolated from Indian Fermented Milk

2017 ◽  
Vol 5 (13) ◽  
Author(s):  
Ni Putu Desy Aryantini ◽  
Jashbhai B. Prajapati ◽  
Tadasu Urashima ◽  
Kenji Fukuda
Keyword(s):  
Real Time ◽  
Genome Sequence ◽  
Single Molecule ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Fermented Milk ◽  
Lactobacillus Fermentum ◽  
Sequencing Technology ◽  
Content Type

ABSTRACT Lactobacillus fermentum MTCC 25067 (formerly TDS030603) is capable of producing a highly viscous slime exopolysaccharide. We report here the complete genome sequence of the strain, which was deciphered by using PacBio single-molecule real-time sequencing technology.

scholarly journals Complete Genome Sequence of Bartonella alsatica Strain IBS 382 (CIP 105477)

2020 ◽  
Vol 9 (34) ◽  
Author(s):  
Arno Thibau ◽  
Tilman G. Schultze ◽  
Wibke Ballhorn ◽  
Volkhard A. J. Kempf
Keyword(s):  
Genome Sequence ◽  
Single Molecule ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Zoonotic Potential ◽  
Smrt Sequencing ◽  
Content Type ◽  
Long Read ◽  
Human Infections ◽  
Future Work

ABSTRACT Bartonella alsatica causes bacteremia in rabbits and, rarely, human infections. Here, we announce the complete and closed genome of B. alsatica IBS 382 (CIP 105477), generated by long-read Pacific Biosciences single-molecule real-time (SMRT) sequencing. The availability of this genome sequence allows future work on understanding the zoonotic potential of this pathogen.

scholarly journals Complete Genome Sequence of Agrococcus sp. Strain SGAir0287, Isolated from Tropical Air Collected in Singapore

2019 ◽  
Vol 8 (32) ◽  
Author(s):  
Kenny J. X. Lau ◽  
Ana Carolina M. Junqueira ◽  
Akira Uchida ◽  
Rikky W. Purbojati ◽  
James N. I. Houghton ◽  
...  
Keyword(s):  
Single Molecule ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Smrt Sequencing ◽  
Entire Genome ◽  
Circular Chromosome ◽  
Protein Coding ◽  
Content Type ◽  
Air Samples ◽  
Protein Coding Genes

Agrococcus sp. strain SGAir0287 was isolated from tropical air samples collected in Singapore. Assembled using single-molecule real-time (SMRT) sequencing and MiSeq reads, the genome consists of one circular chromosome of 3,084,767 bp. The entire genome has 2,870 protein-coding genes, 45 tRNAs, and 3 rRNAs.

scholarly journals Complete Genome Sequence and Methylome Analysis of Deinococcus wulumuqiensis 479

2019 ◽  
Vol 8 (12) ◽  
Author(s):  
Alexey Fomenkov ◽  
Yvette Luyten ◽  
Tamas Vincze ◽  
Brian P. Anton ◽  
Richard J. Roberts ◽  
...  
Keyword(s):  
Single Molecule ◽  
Restriction Enzyme ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Deinococcus Radiodurans ◽  
Complete Sequence ◽  
Smrt Sequencing ◽  
Original Source ◽  
Content Type ◽  
Methylome Analysis

Deinococcus wulumuqiensis 479 (formerly known as Deinococcus radiodurans 479) is the original source strain for the restriction enzyme DrdI. Its complete sequence and full methylome were determined using Pacific Biosciences single-molecule real-time (SMRT) sequencing.

scholarly journals Complete Genome Sequence of Pediococcus pentosaceus Strain GDIAS 001

2020 ◽  
Vol 9 (12) ◽  
Author(s):  
Maopeng Song ◽  
Yongfei Wang ◽  
Xianyong Ma ◽  
Dun Deng
Keyword(s):  
Single Molecule ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Trna Genes ◽  
Smrt Sequencing ◽  
Protein Coding ◽  
Pediococcus Pentosaceus ◽  
Content Type ◽  
Protein Coding Genes ◽  
Rna Genes

Pediococcus pentosaceus strain GDIAS 001 was isolated from a tapioca sample in Guangzou, China. The genome of GDIAS 001 was assembled using single-molecule real-time (SMRT) sequencing, and it contains 1 chromosome of 1.83 Mbp and 1,835 protein-coding genes, 71 RNA genes, and 56 tRNA genes.

scholarly journals Complete Genome Sequence of Mycoplasma pneumoniae Type 2 Reference Strain FH Using Single-Molecule Real-Time Sequencing Technology

2017 ◽  
Vol 5 (8) ◽  
Author(s):  
Heta P. Desai ◽  
Shatavia S. Morrison ◽  
Maureen H. Diaz ◽  
Alvaro J. Benitez ◽  
Bernard J. Wolff ◽  
...  
Keyword(s):  
Mycoplasma Pneumoniae ◽  
Genome Sequence ◽  
Single Molecule ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Reference Strain ◽  
Sequencing Technology ◽  
Content Type ◽  
The Pacific

ABSTRACT Mycoplasma pneumoniae type 2 strain FH was previously sequenced with Illumina (FH-Illumina) and 454 (FH-454) technologies according to Xiao et al. (2015) and Krishnakumar et al. (2010). Comparative analyses revealed differences in genomic content between these sequences, including a 6-kb region absent from the FH-454 submission. Here, we present a complete genome sequence of FH sequenced with the Pacific Biosciences RSII platform.

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