Atomic force microscopy of the erythrocyte membrane in obstructive jaundice

In recent years, many mammalian cells, especially erythrocytes because of simpleness of their membrane surfaces, were widely studied by atomic force microscopy. In our study, diseased erythrocytes were taken from patients of lung cancer, myelodisplastic syndrome (MDS), and so on. We obtained many clear topographical images of numerous erythrocytes, single erythrocyte, and ultramicrostructure of erythrocyte membrane surfaces from normal persons and patients. By studying the red cells of lung cancer patients, we found that many erythrocytes of lung cancer patient have changed into echinocytes. One erythrocyte has 10–20 short projections, most of which, with a mean width of 589.0 nm and a length of 646.7 nm, are on the edge of cell. The projections in the center of echinocytes are lodged and embedded, but in conventional model of echinocytes, the projections in the center stretch outside cell membrane, so a novel model of erythrocytes was designed in our paper. After observation of microstructure of MDS patient's erythrocyte membrane surface, we found that many apertures with different diameters of tens to hundreds nanometers appeared on the surface of cell membrane. It can be concluded that AFM may be widely applied in clinic pathological inspection.

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Morphological changes induced in erythrocyte membrane by the antiepileptic treatment: An atomic force microscopy study

Microscopy Research and Technique ◽

10.1002/jemt.22804 ◽

2016 ◽

Vol 80 (4) ◽

pp. 364-373 ◽

Cited By ~ 1

Author(s):

Bogdan Oprisan ◽

Iuliana Stoica ◽

Mihaela Iuliana Avadanei

Keyword(s):

Atomic Force Microscopy ◽

Erythrocyte Membrane ◽

Morphological Changes ◽

Microscopy Study ◽

Atomic Force Microscopy Study ◽

Antiepileptic Treatment ◽

Force Microscopy ◽

Atomic Force

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Reactive Effect of Low Intensity He-Ne Laser upon Damaged Ultrastructure of Human Erythrocyte Membrane in Fenton System by Atomic Force Microscopy

Acta Biochimica et Biophysica Sinica ◽

10.1111/j.1745-7270.2007.00309.x ◽

2007 ◽

Vol 39 (7) ◽

pp. 484-489 ◽

Cited By ~ 8

Author(s):

Yanhong CUI ◽

Zhouyi GUO ◽

Yanping ZHAO ◽

Ying ZHENG ◽

Yanfang QIAO ◽

...

Keyword(s):

Atomic Force Microscopy ◽

Erythrocyte Membrane ◽

Human Erythrocyte ◽

Human Erythrocyte Membrane ◽

Force Microscopy ◽

Low Intensity ◽

Atomic Force ◽

Reactive Effect ◽

Fenton System

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The how, when, and why of the aging signals appearing on the human erythrocyte membrane: an atomic force microscopy study of surface roughness

Nanomedicine Nanotechnology Biology and Medicine ◽

10.1016/j.nano.2010.06.004 ◽

2010 ◽

Vol 6 (6) ◽

pp. 760-768 ◽

Cited By ~ 54

Author(s):

Marco Girasole ◽

Giuliano Pompeo ◽

Antonio Cricenti ◽

Giovanni Longo ◽

Giovanna Boumis ◽

...

Keyword(s):

Surface Roughness ◽

Atomic Force Microscopy ◽

Erythrocyte Membrane ◽

Human Erythrocyte ◽

Microscopy Study ◽

Human Erythrocyte Membrane ◽

Atomic Force Microscopy Study ◽

Force Microscopy ◽

Atomic Force

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Structure of the Erythrocyte Membrane Skeleton as Observed by Atomic Force Microscopy

Biophysical Journal ◽

10.1016/s0006-3495(98)77926-3 ◽

1998 ◽

Vol 74 (5) ◽

pp. 2171-2183 ◽

Cited By ~ 93

Author(s):

Minoru Takeuchi ◽

Hiroshi Miyamoto ◽

Yasushi Sako ◽

Hideo Komizu ◽

Akihiro Kusumi

Keyword(s):

Atomic Force Microscopy ◽

Erythrocyte Membrane ◽

Membrane Skeleton ◽

Force Microscopy ◽

Atomic Force

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SIMULATION OF MORPHOLOGICAL AND FUNCTIONAL PROPERTIES OF ERYTHROCYTE MEMBRANE

Vestnik of Samara University Natural Science Series ◽

10.18287/2541-7525-2013-19-9.1-177-190 ◽

2017 ◽

Vol 19 (9.1) ◽

pp. 177-190

Author(s):

Yu.S. Nagornov ◽

I.V. Zhilyaev

Keyword(s):

Finite Element Analysis ◽

Experimental Study ◽

Atomic Force Microscopy ◽

Finite Element ◽

Erythrocyte Membrane ◽

Optimization Methods ◽

Element Analysis ◽

Erythrocyte Morphology ◽

Force Microscopy ◽

Atomic Force

The paper presents a model for the calculation of morphofunctional erythrocyte properties. The model represents the erythrocyte as a homogeneous elastic body with elastic depending on the distance to the center of symmetry of the erythrocyte. The data for modeling were taken from the experimental study, which were used by atomic force microscopy (measuring the elasticity of the membrane of erythrocytes and morphology) and the Coulter method. In the developed model, the elasticity of the membrane to change depending on the distance to the center within 1-1,6 kPa. The calculation of the elastic properties is made by two methods - finite element analysis and optimization methods. In the model the dependence of erythrocyte morphology on the membrane pressure was obtained. Pressure difference across the erythrocyte membrane varied in the range of 0,5-2 kPa.

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