Comparative sensitivity of cholinesterases in vertebrates and invertebrates to highly specific organophosphorus inhibitors, diisopropyl fluorophosphate (DFP) and (2-ethoxymethyl phosphoryl thioethyl) ethyl (methyl) sulphonium sulphomethylate (GD-42)

2015 ◽  
Vol 51 (6) ◽  
pp. 471-479 ◽  
Author(s):  
N. E. Basova ◽  
B. N. Kormilitsyn ◽  
A. Yu. Perchenok ◽  
E. V. Rozengart ◽  
V. S. Saakov ◽  
...  
Keyword(s):  
Diisopropyl Fluorophosphate ◽  
Comparative Sensitivity ◽  
Ethyl Methyl ◽  
Organophosphorus Inhibitors

Plasminogen-Activator in Human Early Milk: Its Partial Purification and Characterization

1981 ◽  
Vol 45 (02) ◽  
pp. 121-126 ◽  
Author(s):  
Utako Okamoto ◽  
Noboru Horie ◽  
Yoko Nagamatsu ◽  
Jun-Ichiro Yamamoto
Keyword(s):  
Plasminogen Activator ◽  
Polyacrylamide Gel Electrophoresis ◽  
Gel Filtration ◽  
Partial Purification ◽  
Order Kinetic ◽  
Diisopropyl Fluorophosphate ◽  
Step Procedure ◽  
Activity Band ◽  
C 50 ◽  
Gel Isoelectric Focusing

SummaryMilk plasminogen-activator was partially purified from human transitional milk collected at about 10 days after delivery, by a five-step procedure involving chloroform treatment, ammonium sulfate precipitation, and column chromatography on Sephadex G-150, CM Sephadex C-50 and DEAE Sephadex A-50. This gave milk-activator with a maximum purification factor of about 2,400-fold with respect to the skimmed milk. The CM Sephadex-step preparation showed, on polyacrylamide gel electrophoresis, a single plasminogen-activator activity band located between the bands of albumin and prealbumin of human serum. This preparation exhibited no kinin forming activity. The activator hydrolyzed acetyl-glycyl-L-lysine methyl ester with similar order kinetic constants to urokinase, and was inhibited strongly by diisopropyl-fluorophosphate. The molecular weight of the activator as estimated by gel filtration was approximately 86,000, the isoelectric points as estimated by gel isoelectric focusing were pH 7.2, 6.9 and 6.6, and the activator activity was not quenched by antiurokinase globulin, indicating that the milk-activator is a different entity from urokinase.

SiO2-POCl2 nano-particles promoted the synthesis of novel structure of alkyl 2-oxo-3,4-diaryloxazolidine-5-carboxylate derivatives

2020 ◽  
Vol 17 ◽  
Author(s):  
Mehdi Abaszadeh ◽  
Seyyed Jalal Roudbaraki ◽  
Hossein Yarahmadi ◽  
Majid Ghashang
Keyword(s):  
Nano Particles ◽  
Methyl Ethyl ◽  
Aniline Derivatives ◽  
Ethyl Methyl ◽  
Novel Structure ◽  
Three Component Reaction ◽  
High Yields

: Silica-POCl2 was used as the promoter for the three-component reaction of ethyl/methyl 3-phenyl glycidate, aniline derivatives, and dimethylcarbonate, leads to the synthesis of methyl/ethyl 2-oxo-4-phenyl-3-aryloxazolidine-5-carboxylate derivatives. The desired product was obtained in high yields.

scholarly journals THE pH DEPENDENCE AND DISSOCIATION CONSTANTS OF ESTERASES AND PROTEASES TREATED WITH DIISOPROPYL FLUOROPHOSPHATE

1957 ◽  
Vol 226 (2) ◽  
pp. 867-872 ◽  
Author(s):  
L.A. Mounter ◽  
H.C. Alexander ◽  
Kenneth D. Tuck ◽  
Lien Tien H. Dien
Keyword(s):  
Dissociation Constants ◽  
Ph Dependence ◽  
Diisopropyl Fluorophosphate

scholarly journals APPARENT SPECIFIC INHIBITION OF PLANT ACETYLESTERASE BY DIISOPROPYL FLUOROPHOSPHATE

1947 ◽  
Vol 170 (1) ◽  
pp. 417-418
Author(s):  
E.F. Jansen ◽  
Marvel-Dare Fellows Nutting ◽  
A.K. Balls
Keyword(s):  
Specific Inhibition ◽  
Diisopropyl Fluorophosphate

scholarly journals Binding of 125I-fasciculin to rat brain acetylcholinesterase. The complex still binds diisopropyl fluorophosphate

1993 ◽  
Vol 268 (17) ◽  
pp. 12458-12467
Author(s):  
P. Marchot ◽  
A. Khélif ◽  
Y.H. Ji ◽  
P. Mansuelle ◽  
P.E. Bougis
Keyword(s):  
Rat Brain ◽  
Diisopropyl Fluorophosphate ◽  
Brain Acetylcholinesterase
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