Relative retention analysis in reversed-phase HPLC: The determination, use, and role of the parameter logP in the n-octanol-water system

The influence of exchange of HCOOH (System 2) by phosphoric acid (System 1) for acidification of the “acetonitrile–water” mobile phases for reversed-phase HPLC of anthocyanins was investigated in the framework of relative retention analysis. The differences and similarities of anthocyanins separation were revealed. It has been shown that some common features of the quantitative relationships may be used for preliminary anthocyanins structure differentiation, according to the number of OH-groups in anthocyanidin backbone as well as to a number of saccharide molecules in glycoside radicals in position 3 of the anthocyanin without MS detection.

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Evidence of eosinophil granule major basic protein in human placenta.

Journal of Experimental Medicine ◽

10.1084/jem.170.6.2051 ◽

1989 ◽

Vol 170 (6) ◽

pp. 2051-2063 ◽

Cited By ~ 25

Author(s):

T L Wasmoen ◽

D J McKean ◽

K Benirschke ◽

C B Coulam ◽

G J Gleich

Keyword(s):

Human Placenta ◽

Basic Protein ◽

Gel Filtration ◽

Reversed Phase ◽

Reversed Phase Hplc ◽

Major Basic Protein ◽

Disulfide Linkages ◽

Polyclonal Antisera ◽

Eosinophil Granule

A protein immunochemically related to the eosinophil granule major basic protein (gMBP) is found in increased concentration in the plasma of pregnant women and has been localized to placental trophoblasts by immunofluorescence. Pregnancy MBP (pMBP) is indistinguishable from gMBP in its reactivity with polyclonal antisera and a panel of 14 mouse mAbs. We report the purification of pMBP from human placenta by: (a) affinity chromatography over mAb immobilized on Sepharose, (b) gel filtration in 6 M guanidine.HCl buffer, and (c) reversed-phase HPLC. Purified pMBP and gMBP are biochemically indistinguishable in that both: (a) bind to DNA, (b) polymerize and bind to carrier proteins via disulfide linkages, (c) have a molecular weight of 14,000, (d) have isoelectric points greater than 10.6, (e) comigrate in two-dimensional gels, (f) coelute during reversed-phase HPLC on C18 columns, (g) have identical peptide maps after three different digestions, and (h) have partial amino acid sequence identity. This physicochemical identity has important implications as to the role of pMBP in human placentation.

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Determination of Palladium Ion in River Water by Solvent Extraction with 5-Methyl-1,3,4-Thiodiazole-2-thiol Followed by Reversed Phase HPLC

Journal of Liquid Chromatography &amp Related Technologies ◽

10.1080/10826070802424824 ◽

2008 ◽

Vol 31 (19) ◽

pp. 2988-3000 ◽

Cited By ~ 5

Author(s):

Susumu Ichinoki ◽

Takako Mikami ◽

Youichi Fujii

Keyword(s):

Solvent Extraction ◽

River Water ◽

Reversed Phase ◽

Reversed Phase Hplc ◽

Palladium Ion

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Contextualising landscape perceptions: the role of urban landscape, ecosystem and water system in formation of mental models of a stormwater wetland in Brisbane

Landscape Ecology ◽

10.1007/s10980-021-01250-6 ◽

2021 ◽

Author(s):

Beata A. Sochacka ◽

Joannette J. Bos ◽

Meredith Frances Dobbie

Keyword(s):

Mental Models ◽

Urban Landscape ◽

Water System

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A Reversed-phase HPLC Study of the Complexation of Benzene Derivatives Guest Molecules with 5,17-bis(N-Tolyliminomethyl)-25,27-dipropoxycalix[4]arene in Acetonitrile–Water Solution

Journal of Chemical Research ◽

10.1177/174751989902300136 ◽

1999 ◽

Vol 23 (1) ◽

pp. 60-61

Author(s):

O. I. Kalchenko ◽

A. V. Solovyov ◽

J. Lipkowski ◽

V. I. Kalchenko

Keyword(s):

Stability Constants ◽

Water Solution ◽

Reversed Phase ◽

Benzene Derivatives ◽

Reversed Phase Hplc ◽

Guest Molecules ◽

Hplc Study

Stability constants of the host–guest complexes of 5,17-bis( N-tolyliminomethyl)-25,27-dipropoxycalix[4]arene with benzene derivatives were determined by reversed-phase HPLC in acetonitrile–water solution.

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