scholarly journals The Role of Pore Size and Stationary Phase Composition in Preventing Aqueous-Induced Retention Time Loss in Reversed-Phase HPLC

2004 ◽  
Vol 42 (2) ◽  
pp. 100-106 ◽  
Author(s):  
B. A. Bidlingmeyer ◽  
A. D. Broske
Keyword(s):  
Phase Composition ◽  
Stationary Phase ◽  
Pore Size ◽  
Retention Time ◽  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
Time Loss ◽  
Stationary Phase Composition

scholarly journals Reversed‐phase HPLC enantioseparation of pantoprazole using a teicoplanin aglycone stationary phase—Determination of the enantiomer elution order using HPLC‐CD analyses

Chirality ◽  
2019 ◽  
Vol 32 (2) ◽  
pp. 158-167
Author(s):  
Lajos Attila Papp ◽  
Mohammadhassan Foroughbakhshfasaei ◽  
Béla Fiser ◽  
Péter Horváth ◽  
Eszter Kiss ◽  
...  
Keyword(s):  
Stationary Phase ◽  
Reversed Phase ◽  
Elution Order ◽  
Reversed Phase Hplc ◽  
Phase Determination

scholarly journals Identification, Mapping and Relative Quantitation of SARS-CoV-2 Spike Glycopeptides by Mass-Retention Time Fingerprinting

2020 ◽  
Author(s):  
R. Chalk ◽  
W. Greenland ◽  
T. Moreira ◽  
J. Coker ◽  
S.M.M Mukhopadhyay ◽  
...  
Keyword(s):  
Mass Spectrometer ◽  
Retention Time ◽  
Analytical Method ◽  
High Efficiency ◽  
Reversed Phase ◽  
Resolving Power ◽  
Spike Protein ◽  
Reversed Phase Hplc ◽  
Robust Identification ◽  
Relative Quantitation

AbstractWe describe a novel analytical method for rapid and robust identification, mapping and relative quantitation of glycopeptides from SARS-CoV-2 Spike protein. The method may be executed using any LC-TOF mass spectrometer, requires no specialised knowledge of glycan analysis and makes use of the differential resolving power of reversed phase HPLC. While this separation technique resolves peptides with high efficiency, glycans are resolved poorly, if at all. Consequently, glycopeptides consisting of the same peptide bearing different glycan structures will all possess very similar retention times and co-elute. While this has previously been viewed as a disadvantage, we show that shared retention time can be used to map multiple glycan species to the same peptide and location. In combination with MSMS and pseudo MS3, we have constructed a detailed mass-retention time database for Spike. This database allows any ESI-TOF equipped lab to reliably identify and quantify spike glycans from a single overnight elastase protein digest in less than 90 minutes.

scholarly journals Separation and lipophilicity of some new steroid derivatives in normal- and reversed-phase high performance liquid chromatography

2011 ◽  
Vol 17 (4) ◽  
pp. 535-542 ◽  
Author(s):  
Marijana Acanski ◽  
Djura Vujuc ◽  
Suzana Jovanovic-Santa
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Stationary Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Log P ◽  
Reversed Phase Hplc ◽  
Mobile Phases

The separation ability and retention of normal and reversed phase HPLC with, respectively, three non-polar and two polar mobile phases, have been studied by measuring the retention constants of a series of newly synthesized estrone derivatives. The separation ability and retention are discussed in terms of the nature of the solute, eluent and stationary phase. Good correlation was found between the retention constants log k0 of newly synthesized estrone derivatives obtained on C-18 column and log P calculated by different methods.

scholarly journals Role of conformational changes in the elution of proteins from reversed-phase HPLC columns.

1986 ◽  
Vol 83 (12) ◽  
pp. 4268-4272 ◽  
Author(s):  
G. E. Katzenstein ◽  
S. A. Vrona ◽  
R. J. Wechsler ◽  
B. L. Steadman ◽  
R. V. Lewis ◽  
...  
Keyword(s):  
Conformational Changes ◽  
Reversed Phase ◽  
Reversed Phase Hplc

Correlation of the Dynamic Behavior ofn-Alkyl Ligands of the Stationary Phase with the Retention Times of Paracelsin Peptides in Reversed Phase HPLC

1989 ◽  
Vol 28 (3) ◽  
pp. 327-329 ◽  
Author(s):  
Betting Pfleiderer ◽  
Klaus Albert ◽  
Ernst Bayer ◽  
Klaus D. Lork ◽  
Klaus K. Unger ◽  
...  
Keyword(s):  
Stationary Phase ◽  
Dynamic Behavior ◽  
Reversed Phase ◽  
Reversed Phase Hplc
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