scholarly journals Regularities of Anthocyanins Retention in RP HPLC for “Water–Acetonitrile–Phosphoric Acid” Mobile Phases

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
V. I. Deineka ◽  
L. A. Deineka ◽  
I. I. Saenko
Keyword(s):  
Phosphoric Acid ◽  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
Oh Groups ◽  
Common Features ◽  
Relative Retention ◽  
Rp Hplc ◽  
Mobile Phases ◽  
System 2 ◽  
System 1

The influence of exchange of HCOOH (System 2) by phosphoric acid (System 1) for acidification of the “acetonitrile–water” mobile phases for reversed-phase HPLC of anthocyanins was investigated in the framework of relative retention analysis. The differences and similarities of anthocyanins separation were revealed. It has been shown that some common features of the quantitative relationships may be used for preliminary anthocyanins structure differentiation, according to the number of OH-groups in anthocyanidin backbone as well as to a number of saccharide molecules in glycoside radicals in position 3 of the anthocyanin without MS detection.

Hydrophobicity parameters (Log P) of glycoconjugated porphyrins for photodynamic therapy evaluated by reversed phase HPLC

2004 ◽  
Vol 08 (11) ◽  
pp. 1289-1292 ◽  
Author(s):  
Shiho Hirohara ◽  
Makoto Obata ◽  
Shun-ichiro Ogura ◽  
Ichiro Okura ◽  
Suguru Higashida ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Photodynamic Therapy ◽  
High Performance ◽  
Reversed Phase ◽  
Log P ◽  
P Value ◽  
Reversed Phase Hplc ◽  
Free Base ◽  
P Values ◽  
Rp Hplc

Photodynamic therapy (PDT) is one of the most effective treatments for cancer. We synthesized and characterized a series of 16 glycoconjugated porphyrins using a modification of the Lindsey method in the presence of Zn ( OAc )2·2 H 2 O as a template. The Zn 2+ ion template strategy improved the yield about 3-fold in the case of meta-substituted tetraphenylporphyrins, and free-base porphyrins were obtaind almost quantitatively by demetalation with 4 M HCl . The hydrophobicity parameter (Log P) of these porphyrins was evaluated by reversed phase high-performance liquid chromatography (RP-HPLC). The Log P values ranged from +4.8 to +7.8, so that most of the compounds are amphiphilic. The effect of the glycopyranosyl unit on the hydrophobicity of these compounds is discussed on the basis of the Log P value.

scholarly journals Application of a reversed-phase HPLC method for quantitative p-coumaric acid analysis in wine

OENO One ◽  
2010 ◽  
Vol 44 (2) ◽  
pp. 117
Author(s):  
Dominique Salameh ◽  
Cédric Brandam ◽  
Toufic Rizk ◽  
Roger Lteif ◽  
Pierre Strehaiano
Keyword(s):  
Mobile Phase ◽  
Chromatographic Method ◽  
Red Wine ◽  
Reversed Phase ◽  
Hplc Method ◽  
Coumaric Acid ◽  
Reversed Phase Hplc ◽  
Mobile Phase Composition ◽  
Rp Hplc ◽  
Synthetic Media

<p style="text-align: justify;"><strong>Aims</strong>: This paper presents a rapid chromatographic method to monitor the concentration of <em>p</em>-coumaric acid in wine and in bioconversion studies.</p><p style="text-align: justify;"><strong>Methods and results</strong>: RP-HPLC method was validated in synthetic wine medium and in natural red wine. Mobile phase composition was water 77%, acetonitrile 23%. Formic acid was added to control pH at 3.5. The flow was 0.7 mL/min and the temperature 30 °C. The detection was done using UV at 305 nm. The linearity range was validated between 0.5 and 15 mg/L. The resolution was respectively 5.35 and 2.99. The detection and quantification limits were 0.01 mg/L and 0.04 mg/L. This method was used to study <em>p</em>-coumaric acid bioconversion into 4-ethylphenol and 4-vinylphenol, and to study this acid adsorption in enological conditions.</p><p style="text-align: justify;"><strong>Conclusions</strong>: This paper presented a simple HPLC method to monitor the concentration of <em>p</em>-coumaric acid in synthetic media and natural wine. It was used to study the <em>p</em>-coumaric acid bioconversion rates and mechanism.</p><p style="text-align: justify;"><strong>Significance and impact of the study</strong>: This method is useful to monitor <em>p</em>-coumaric acid concentration, which helps to predict amounts of 4-ethylphenol or 4-vinylphenol that can be produced in wine. This method can be helpful to control undesirable phenolic flavors potential in wine.</p>

scholarly journals Separation and lipophilicity of some new steroid derivatives in normal- and reversed-phase high performance liquid chromatography

2011 ◽  
Vol 17 (4) ◽  
pp. 535-542 ◽  
Author(s):  
Marijana Acanski ◽  
Djura Vujuc ◽  
Suzana Jovanovic-Santa
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Stationary Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Log P ◽  
Reversed Phase Hplc ◽  
Mobile Phases

The separation ability and retention of normal and reversed phase HPLC with, respectively, three non-polar and two polar mobile phases, have been studied by measuring the retention constants of a series of newly synthesized estrone derivatives. The separation ability and retention are discussed in terms of the nature of the solute, eluent and stationary phase. Good correlation was found between the retention constants log k0 of newly synthesized estrone derivatives obtained on C-18 column and log P calculated by different methods.

scholarly journals Plasma amino-acid determinations by reversed-phase HPLC: Improvement of the orthophthalaldehyde method and comparison with ion exchange chromatography

1992 ◽  
Vol 14 (4) ◽  
pp. 145-149 ◽  
Author(s):  
Frédéric Ziegler ◽  
Jacques Le Boucher ◽  
Colette Coudray-Lucas ◽  
Luc Cynober
Keyword(s):  
Amino Acid ◽  
Ion Exchange ◽  
Reversed Phase ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Reversed Phase Hplc ◽  
Suitable Alternative ◽  
Free Tryptophan ◽  
Rp Hplc ◽  
Low Concentrations

RP-HPLC with automated pre-column OPA derivatization is clearly a suitable alternative for assaying physiological AA and may be particularly useful for AA present at low concentrations (free tryptophan, plasma 3-methylhistidine).

TLC-Densitometric and RP-HPLC Methods for Simultaneous Determination of Dexamethasone and Chlorpheniramine Maleate in the Presence of Methylparaben and Propylparaben

2017 ◽  
Vol 100 (1) ◽  
pp. 51-58
Author(s):  
Nehal F Farid ◽  
Ibrahim A Naguib ◽  
Radwa S Moatamed ◽  
Mohamed R El Ghobashy
Keyword(s):  
Particle Size ◽  
Quantitative Determination ◽  
Mobile Phase ◽  
Ammonium Acetate ◽  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
Chlorpheniramine Maleate ◽  
Ammonium Acetate Buffer ◽  
Rp Hplc

Abstract Validated simple, sensitive, and highly selective methods are applied for the quantitative determination ofdexamethasone and chlorpheniramine maleate in the presence of their reported preservatives (methylparaben and propylparaben), whether in pure forms or in pharmaceutical formulation. TLC is the first method, in which dexamethasone, chlorpheniramine maleate, methylparaben, and propylparaben are separated on silica gel TLC F254 plates using hexane–acetone–ammonia (5.5 + 4.5 + 0.5, v/v/v) as the developing phase. Separated bands are scanned at 254 nm over a concentration range of 0.1–1.7 and 0.4–2.8 μg/band, with mean ± SD recoveries of 99.12 ± 0.964 and 100.14 ± 0.962%, for dexamethasone and chlorpheniramine maleate, respectively. Reversed-phase HPLC is the second method, in which a mixture of dexamethasone and chlorpheniramine maleate, methylparaben, and propylparaben is separated on a reversed-phase silica C18 (5 μm particle size, 250 mm, 4.6 mm id) column using 0.1 M ammonium acetate buffer–acetonitrile (60 + 40, v/v, pH 3) as the mobile phase. The drugs were detected at 220 nm over a concentration range of 5–50 μg/mL, 2–90 μg/mL, 4–100 μg/mL, and 7–50μg/mL, with mean ± SD recoveries of 100.85 ± 0.905, 99.67 ± 1.281, 100.20 ± 0.906, and 99.81 ± 0.954%, for dexamethasone, chlorpheniramine maleate, methylparaben paraben, and propylparaben, respectively. The advantages of the suggested methods over previously reported methods are the ability to detect lower concentrations of the main drugs and to show better resolution of interfering preservatives; hence, these methods could be more reliable for routine QC analyses.

scholarly journals Quantitative Determination of Ellagic Acid and Gallic Acid in Geum Rivale L. and G. Urbanum L.

2015 ◽  
Vol 56 (2) ◽  
pp. 74-78 ◽  
Author(s):  
Aleksandra Owczarek ◽  
Monika Anna Olszewska ◽  
Jan Gudej
Keyword(s):  
Medicinal Plants ◽  
Gallic Acid ◽  
Ellagic Acid ◽  
Biological Activities ◽  
Reversed Phase ◽  
Herbal Remedies ◽  
Reversed Phase Hplc ◽  
Hydrolyzable Tannins ◽  
Rp Hplc

Abstract Hydrolyzable tannins and products of their hydrolysis, ellagic acid (EA) and gallic acid (GA), are important con-stituents of many medicinal plants and exhibit various biological activities. Geum rivale and G. urbanum are traditional herbal remedies rich in tannin compounds. The aim of the study was to quantitate free and total EA and GA in aerial and underground parts of G. rivale and G. urbanum. After optimization of extraction, both compounds were quantitated by reversed phase HPLC (RP-HPLC). EA was more abundant than GA in the inves-tigated material, and underground parts of G. rivale were the richest source of total EA and GA.

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