Role of conformational changes in the elution of proteins from reversed-phase HPLC columns.

A protein immunochemically related to the eosinophil granule major basic protein (gMBP) is found in increased concentration in the plasma of pregnant women and has been localized to placental trophoblasts by immunofluorescence. Pregnancy MBP (pMBP) is indistinguishable from gMBP in its reactivity with polyclonal antisera and a panel of 14 mouse mAbs. We report the purification of pMBP from human placenta by: (a) affinity chromatography over mAb immobilized on Sepharose, (b) gel filtration in 6 M guanidine.HCl buffer, and (c) reversed-phase HPLC. Purified pMBP and gMBP are biochemically indistinguishable in that both: (a) bind to DNA, (b) polymerize and bind to carrier proteins via disulfide linkages, (c) have a molecular weight of 14,000, (d) have isoelectric points greater than 10.6, (e) comigrate in two-dimensional gels, (f) coelute during reversed-phase HPLC on C18 columns, (g) have identical peptide maps after three different digestions, and (h) have partial amino acid sequence identity. This physicochemical identity has important implications as to the role of pMBP in human placentation.

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Role of spectrin in membrane fusion and in shape change of human erythrocyte ghost

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100082509 ◽

1978 ◽

Vol 36 (2) ◽

pp. 328-329

Author(s):

Hideo Hayashi ◽

Yoshikazu Hirai ◽

John T. Penniston

Keyword(s):

Conformational Changes ◽

Human Erythrocyte ◽

Shape Change ◽

Membrane Surface ◽

Slight Modification ◽

Active Role ◽

Main Role ◽

Bank Blood ◽

Human Erythrocyte Ghost

Spectrin is a membrane associated protein most of which properties have been tentatively elucidated. A main role of the protein has been assumed to give a supporting structure to inside of the membrane. As reported previously, however, the isolated spectrin molecule underwent self assemble to form such as fibrous, meshwork, dispersed or aggregated arrangements depending upon the buffer suspended and was suggested to play an active role in the membrane conformational changes. In this study, the role of spectrin and actin was examined in terms of the molecular arrangements on the erythrocyte membrane surface with correlation to the functional states of the ghosts.Human erythrocyte ghosts were prepared from either freshly drawn or stocked bank blood by the method of Dodge et al with a slight modification as described before. Anti-spectrin antibody was raised against rabbit by injection of purified spectrin and partially purified.

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Determination of Palladium Ion in River Water by Solvent Extraction with 5-Methyl-1,3,4-Thiodiazole-2-thiol Followed by Reversed Phase HPLC

Journal of Liquid Chromatography &amp Related Technologies ◽

10.1080/10826070802424824 ◽

2008 ◽

Vol 31 (19) ◽

pp. 2988-3000 ◽

Cited By ~ 5

Author(s):

Susumu Ichinoki ◽

Takako Mikami ◽

Youichi Fujii

Keyword(s):

Solvent Extraction ◽

River Water ◽

Reversed Phase ◽

Reversed Phase Hplc ◽

Palladium Ion

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A Reversed-phase HPLC Study of the Complexation of Benzene Derivatives Guest Molecules with 5,17-bis(N-Tolyliminomethyl)-25,27-dipropoxycalix[4]arene in Acetonitrile–Water Solution

Journal of Chemical Research ◽

10.1177/174751989902300136 ◽

1999 ◽

Vol 23 (1) ◽

pp. 60-61

Author(s):

O. I. Kalchenko ◽

A. V. Solovyov ◽

J. Lipkowski ◽

V. I. Kalchenko

Keyword(s):

Stability Constants ◽

Water Solution ◽

Reversed Phase ◽

Benzene Derivatives ◽

Reversed Phase Hplc ◽

Guest Molecules ◽

Hplc Study

Stability constants of the host–guest complexes of 5,17-bis( N-tolyliminomethyl)-25,27-dipropoxycalix[4]arene with benzene derivatives were determined by reversed-phase HPLC in acetonitrile–water solution.

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Role of conformational heterogeneity in ligand recognition by viral RNA molecules

Physical Chemistry Chemical Physics ◽

10.1039/d1cp00679g ◽

2021 ◽

Author(s):

Lev Levintov ◽

Harish Vashisth

Keyword(s):

Conformational Changes ◽

Ribonucleic Acid ◽

Viral Rna ◽

Ligand Recognition ◽

Conformational Heterogeneity ◽

Rna Molecules ◽

Environmental Stimuli

Ribonucleic acid (RNA) molecules are known to undergo conformational changes in response to various environmental stimuli including temperature, pH, and ligands. In particular, viral RNA molecules are a key example...

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