Mosaic expression pattern of the nptII gene in transgenic tobacco plants Nu 21

2007 ◽  
Vol 43 (7) ◽  
pp. 780-790 ◽  
Author(s):  
T. V. Marenkova (Novoselia) ◽  
E. V. Deineko ◽  
V. K. Shumny
Keyword(s):  
Transgenic Tobacco ◽  
Expression Pattern ◽  
Nptii Gene ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Mosaic Expression

scholarly journals Effects of salt stress on proline content, expression of delta-1-pyrroline-5-carboxylate synthetase, and activities of catalase and ascorbate peroxidase in transgenic tobacco plants

2009 ◽  
Vol 46 (2) ◽  
pp. 63-75 ◽  
Author(s):  
Roya Razavizadeh ◽  
Ali Ehsanpour
Keyword(s):  
Salt Stress ◽  
Transgenic Plants ◽  
Transgenic Tobacco ◽  
Expression Pattern ◽  
Ascorbate Peroxidase ◽  
Proline Content ◽  
35S Promoter ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Moth Bean

Effects of salt stress on proline content, expression of delta-1-pyrroline-5-carboxylate synthetase, and activities of catalase and ascorbate peroxidase in transgenic tobacco plantsIn arid and semiarid regions, soil salinity limits crop production. Proline accumulation in transgenic plants results in increased stress tolerance, but the underlying mechanism was unclear. To elucidate it, effects of salt stress on the expression pattern of Δ1-pyrroline-5-carboxylate synthetase (P5CS), proline content, catalase (CAT), and ascorbate peroxidase (APX) activities were analyzed in transgenic tobacco (Nicotiana tabacumcv. Wisconsin). Transgenic tobacco plants containing CaMV 35S promoter and theP5CSgene from moth bean (Vigna aconitifolia), linked to theNPTIIgene, were culturedin vitrowith or without 300 mM NaCl. The expression pattern ofP5CSwas evaluated using semiquantitative RT-PCR (reverse transcription-polymerase chain reaction). Time-course experiments showed an increase in proline content after 4 h of the treatment. The level ofP5CStranscripts was increased significantly in leaves and roots of transgenic plants after 24 and 48 h of treatment. This rise in transcripts was concomitant with the highest increase in proline content. In addition, CAT and APX activities increased under salt stress, and their highest activities were observed after 24 and 48 h of NaCl treatment. These results suggest thatP5CSis an inducible gene regulating the activities of CAT and APX and the accumulation of proline in plants subjected to salt stress.

scholarly journals Molecular Analysis and Effectiveness Assay of AV1 Gene in Transgenic Tobacco for Resistance to Begomovirus

2016 ◽  
Vol 8 (2) ◽  
pp. 45
Author(s):  
Tri Joko Santoso ◽  
Muhammad Herman ◽  
Sri H. Hidayat ◽  
Hajrial Aswidinnoor ◽  
Sudarsono Sudarsono
Keyword(s):  
Transgenic Plant ◽  
Transgenic Tobacco ◽  
Tobacco Plant ◽  
Universal Primers ◽  
Protein Accumulation ◽  
Gene Probe ◽  
Nptii Gene ◽  
Transgenic Tobacco Plants ◽  
Resistance Response ◽  
Tobacco Plants

<p>Genetic transformation<br />of tobacco plant using AV1 gene was conducted at<br />the previously experiment and generated transgenic tobacco<br />plants positively carrying the selectable marker nptII gene.<br />The objectives of this experiment were to (1) analyze the<br />presence of Begomovirus AV1 gene in T0 generation putative<br />transgenic tobacco plants using PCR technique with specific<br />primers and its correlation with resistance phenotype, (2)<br />analyze the integration and copy number of the transgene in<br />T0 generation putative transgenic tobacco plants and its<br />correlation with resistance response, (3) screen the T0<br />generation putative transgenic tobacco plants with the target<br />virus infection and to detect the presence of the virus in the<br />transgenic plant tissue using universal primers. PCR<br />detection of AV1 gene in tobacco transgenic was conducted<br />by using specific primer for Begomovirus AV1 gene.<br />Meanwhile, Southern Blot analysis was conducted by using<br />the AV1 gene probe. The effectiveness of AV1 gene in<br />tobacco transgenic was tested by inoculation of target virus<br />using whiteflies vector. Result of the experiments showed<br />that there was a positive correlation between the presence<br />of the AV1 transgene in T0 generation putative transgenic<br />tobacco plants and the resistant phenotype. Transgenic<br />plants with a single copy integration of the transgene<br />exhibited more resistant than the multiple copy one. and<br />non transgenic plant. The resistance as a result of AV1 gene<br />expression was indicated with no symptom in T0 generation<br />transgenic tobacco plants and the accumulation of the virus<br />in the transgenic plants tissue. Northern and Western<br />hybridization analysis need to be perfomed for investigating<br />the presence of mRNA or protein accumulation so that the<br />resistance mechanism of the AV1 gene could be explained<br />more detail.</p>

Production of an active recombinant thrombomodulin derivative in transgenic tobacco plants and suspension cells

2005 ◽  
Vol 14 (3) ◽  
pp. 251-259 ◽  
Author(s):  
Helga Schinkel ◽  
Andreas Schiermeyer ◽  
Raphael Soeur ◽  
Rainer Fischer ◽  
Stefan Schillberg
Keyword(s):  
Transgenic Tobacco ◽  
Transgenic Tobacco Plants ◽  
Suspension Cells ◽  
Tobacco Plants ◽  
Recombinant Thrombomodulin

scholarly journals Overexpression of the pitaya phosphoethanolamine N-methyltransferase gene (HpPEAMT) enhanced simulated drought stress in tobacco

2021 ◽  
Author(s):  
Ai-Hua Wang ◽  
Lan Yang ◽  
Xin-Zhuan Yao ◽  
Xiao-Peng Wen
Keyword(s):  
Drought Stress ◽  
Stress Response ◽  
Transgenic Plants ◽  
Drought Tolerance ◽  
Transgenic Tobacco ◽  
Sequence Similarity ◽  
Amino Acid Sequence Similarity ◽  
Transgenic Tobacco Plants ◽  
Wild Type ◽  
Tobacco Plants

AbstractPhosphoethanolamine N-methyltransferase (PEAMTase) catalyzes the methylation of phosphoethanolamine to produce phosphocholine and plays an important role in the abiotic stress response. Although the PEAMT genes has been isolated from many species other than pitaya, its role in the drought stress response has not yet been fully elucidated. In the present study, we isolated a 1485 bp cDNA fragment of HpPEAMT from pitaya (Hylocereus polyrhizus). Phylogenetic analysis showed that, during its evolution, HpPEAMT has shown a high degree of amino acid sequence similarity with the orthologous genes in Chenopodiaceae species. To further investigate the function of HpPEAMT, we generated transgenic tobacco plants overexpressing HpPEAMT, and the transgenic plants accumulated significantly more glycine betaine (GB) than did the wild type (WT). Drought tolerance trials indicated that, compared with those of the wild-type (WT) plants, the roots of the transgenic plants showed higher drought tolerance ability and exhibited improved drought tolerance. Further analysis revealed that overexpression of HpPEAM in Nicotiana tabacum resulted in upregulation of transcript levels of GB biosynthesis-related genes (NiBADH, NiCMO and NiSDC) in the leaves. Furthermore, compared with the wild-type plants, the transgenic tobacco plants displayed a significantly lower malondialdehyde (MDA) accumulation and higher activities of the superoxide dismutase (SOD) and peroxidase (POD) antioxidant enzymes under drought stress. Taken together, our results suggested that HpPEAMT enhanced the drought tolerance of transgenic tobacco.

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