Development of methods of the gas chromatographic determination of phenylcarboxylic acids in blood serum and their adaptation to clinical laboratory conditions

2015 ◽  
Vol 70 (4) ◽  
pp. 495-501 ◽  
Author(s):  
V. V. Moroz ◽  
N. V. Beloborodova ◽  
A. Yu. Bedova ◽  
A. I. Revel’skii ◽  
M. L. Getsina ◽  
...  
Keyword(s):  
Blood Serum ◽  
Clinical Laboratory ◽  
Chromatographic Determination ◽  
Laboratory Conditions ◽  
Phenylcarboxylic Acids

Liquid-chromatographic determination of serotonin in serum and plasma.

1978 ◽  
Vol 24 (9) ◽  
pp. 1509-1514 ◽  
Author(s):  
S Sasa ◽  
C L Blank ◽  
D C Wenke ◽  
C A Sczupak
Keyword(s):  
High Performance ◽  
Clinical Laboratory ◽  
Chromatographic Determination ◽  
Present Scheme ◽  
Long Term Storage ◽  
Significant Difference ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract Using high-performance liquid chromatography with electrochemical detection, we determined serotonin in plasma from parkinsonian patients being treated with L-3,4-dihydroxyphenylalanine or N-(DL-seryl)-N'(2,3,4-trihydroxybenzyl)hydrochloride plus L-3,4-dihydroxyphenylalanine ("Sinemet") and in serum from a blood bank, from "normal" persons, and a pooled specimen from a hospital clinical laboratory. The values obtained for the two groups of Parkinson's disease patients showed no significant difference. Long-term storage on solid CO2 was xhown to be an adequate technique for preserving samples. The mean (+/-SEAM) normal value obtained for serotonin in serum was 146 +/- 46 microgram/liter (n = 23), a result in harmony with that previously obtained [Clin. Chem. 20, 812 (1974)] by fluorometry. In comparison to other methods for measurement of serotonin in serum or plasma, we believe that the present scheme offers greater selectivity, sensitivity, and precision.

High-performance thin-layer chromatographic determination of psychopharmacologic agents in blood serum

1977 ◽  
Vol 142 ◽  
pp. 399-409 ◽  
Author(s):  
D.C. Fenimore ◽  
C.J. Meyer ◽  
C.M. Davis ◽  
F. Hsu ◽  
A. Zlatkis
Keyword(s):  
Thin Layer ◽  
Blood Serum ◽  
High Performance ◽  
Chromatographic Determination

Phenolphthalein Monophosphate as a Substrate for Serum Alkaline Phosphatase

1966 ◽  
Vol 12 (10) ◽  
pp. 701-708 ◽  
Author(s):  
J H Wilkinson ◽  
Ann V Vodden
Keyword(s):  
Alkaline Phosphatase ◽  
Serum Concentration ◽  
Inorganic Phosphate ◽  
Clinical Laboratory ◽  
Serum Alkaline Phosphatase ◽  
Routine Procedure ◽  
Laboratory Conditions ◽  
Straight Line ◽  
Routine Clinical Laboratory

Abstract The procedure for serum alkaline phosphatase determination employing phenolphthalein monophosphate as substrate was evaluated under routine clinical laboratory conditions. A straight-line response to increasing serum concentration was observed, and the reaction was shown to be Mg++ dependent. The method proved to be extremely simple, and the results obtained correlated well with those given by a standard routine procedure involving determination of the liberated inorganic phosphate.

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