Optical Sensors on the Basis of a Polyelectrolyte Peroxidase–Chitosan Complex for the Determination of Biologically Active Compounds

2019 ◽  
Vol 74 (1) ◽  
pp. 41-57 ◽  
Author(s):  
I. A. Veselova ◽  
T. N. Shekhovtsova
2014 ◽  
Vol 60 (3) ◽  
pp. 34-43 ◽  
Author(s):  
Katarzyna Bączek

Summary Eight species of Eleutherococcus genus grown at Rogów Arboretum collection were compared in respect of biologically active compounds accumulation, i.e. eleutheroside B and E, phenolic acids and sterols. For the determination of content of these compounds in underground organs and stem bark high performance liquid chromatography was applied. The highest content of the sum of eleutherosides B and E was observed in underground organs and stem bark of E. leucorrhizus (322.0 and 300.8 mg × 100 g-1, respectively) and E. nodiflorus (218.9 and 363.5 mg × 100g-1, respectively). In the raw materials from E. senticosus, the content of these compounds was significantly lower (177.4 and 159.3 mg × 100 g-1, respectively). E divaricatus and E. setchuenensis were characterized by the lowest accumulation of these compounds in underground organs whereas E. divaricatus, E. sessiliflorus and E. giraldii - in stem bark. Four phenolic acids were identified in the investigated species, namely: chlorogenic, rosmarinic, ferulic and caffeic acids. The main phenolic acid in the analyzed species was chlorogenic acid. The content of this compound in underground organs varied from 102.1 (E. henryi) to 958.7 mg × 100 g-1 (E. leucorrhizus) and in stem bark from 26.7 (E. giraldii) to 542.5 mg × 100 g-1 (E. setchuenensis). The content of identified sterol compounds (sitosterol 3-O-β-D-glucoside /eleutheroside A / campesterol, stigmasterol) was relatively low, but higher in underground organs in comparison with stem bark.


2015 ◽  
Vol 46 (3) ◽  
pp. 201-212 ◽  
Author(s):  
Justyna Walczak ◽  
Szymon Bocian ◽  
Tadeusz Trziszka ◽  
Bogusław Buszewski

2014 ◽  
Vol 1073-1076 ◽  
pp. 122-126
Author(s):  
Qiao Yang

In this study, a novel microwave-assisted extraction (MAE) method has been developed for the extraction and determination of the major biologically active compounds, crocin and its seven trans-cis analogues in a traditional Chinese medicine (TCM), gardenia fruits (Gardenia jasminoides Ellis) with analysis by reversed phase HPLC. Sudan I [1-phenylazo-2-naphthol] was selected as the internal standard. The results of quantitative determination of seven crocin analogues in six commercial gardenia fruits samples show that MAE–HPLC is a simple, rapid, low cost and reliable method for the determination of biologically active compounds, crocin analogues in TCM, and also a potential and powerful tool for quality assessment of gardenia fruits (Gardenia jasminoides Ellis).


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