Influence of genotype, explant type, and component of culture medium on in vitro callus induction and shoot organogenesis of tomato (Solanum lycopersicum L.)

2014 ◽  
Vol 41 (6) ◽  
pp. 512-521 ◽  
Author(s):  
M. R. Khaliluev ◽  
L. R. Bogoutdinova ◽  
G. B. Baranova ◽  
E. N. Baranova ◽  
P. N. Kharchenko ◽  
...  
Keyword(s):  
Solanum Lycopersicum ◽  
Callus Induction ◽  
Culture Medium ◽  
Shoot Organogenesis ◽  
Explant Type ◽  
Solanum Lycopersicum L

scholarly journals Induction and Growth Kinetics Callus of Tomato (Solanum lycopersicum)

2020 ◽  
Vol 12 (1) ◽  
pp. 35-41 ◽  
Author(s):  
Arkan Setiaji ◽  
RR Rifka Annisa ◽  
Rumiyati Rumiyati ◽  
Endang Semiarti
Keyword(s):  
Solanum Lycopersicum ◽  
Growth Kinetics ◽  
Callus Induction ◽  
Dry Weight ◽  
Growth Efficiency ◽  
Ms Medium ◽  
Solanum Lycopersicum L ◽  
Absolute Growth ◽  
Embedded Method

Plant callus extracts are potential to be developed as ingredient in skincare products. Tomato callus is supposed to contain protein-derivatives and or other components such as secondary metabolites that play a role in skin regeneration. Therefore the production of calli is important to be studied for callus sustainable supply. This research aims to obtain optimum medium for callus induction and to analyze tomato callus development anatomically. In vitro culture response was assessed in tomato plant (Solanum lycopersicum L. ‘Permata’) for optimum callus induction. Seeds were grown on ¼ MS medium for 10-15 days. Hypocotyl was excised and cultured on MS medium + 2 mg/l 2,4-D for 15 days as the explants for callus induction. Callus was transferred to MS medium with 8 variations of PGRs including the combination of BAP + NAA, and 2,4-D. Both fresh and dry weight was measured every 5 days over 60 days to establish the growth kinetics and growth efficiency of callus. Anatomic characters of calli were examined through paraffin-embedded method. The result showed of MS medium supplemented with 2.0 mg/l NAA and 0.2 mg/l BAP is optimum for tomato callus induction, based on highest number of the absolute growth rate on fresh weight (73.77% per day), dry weight (3.84% per day), and callus initiation time (5.56 days) achieved by the medium. Cells in the ground tissue of tomato hypocotyl are competent to be dedifferentiated into a callus. This research results were expected to find out suitable methods for tomato callus production in preparation for skincare uses.

Морфо-биологическая и цитологическая характеристика регенерирующих корней томата ( Solanum lycopersicum L.) сорта “Рекордсмен” в условиях NaCl-засоления in vitro

Tsitologiya ◽  
2019 ◽  
Vol 61 (12) ◽  
pp. 998-1013
Author(s):  
Л. Р. Богоутдинова ◽  
Е. Н. Баранова ◽  
Г. Б. Баранова ◽  
Н. В. Кононенко ◽  
Е. М. Лазарева ◽  
...  
Keyword(s):  
Solanum Lycopersicum ◽  
Solanum Lycopersicum L

Callus Induction and Shoot Organogenesis in Two Sugar Beet (Beta vulgaris L.) Breeding Lines in vitro Cultured

2013 ◽  
Vol 12 (4) ◽  
pp. 168-178 ◽  
Author(s):  
Farhad Taghipour ◽  
Narges Janalizade ◽  
Maryam Eshrati ◽  
Taraneh Hassanzade ◽  
Fahrul Huyop
Keyword(s):  
Sugar Beet ◽  
Beta Vulgaris ◽  
Callus Induction ◽  
Shoot Organogenesis ◽  
Breeding Lines

Cytokinin-cytokinin interaction ameliorates the callus induction and plant regeneration of tomato (Solanum lycopersicum Mill.)

2012 ◽  
Vol 60 (1) ◽  
pp. 47-55 ◽  
Author(s):  
A. Ali ◽  
T. Yossef ◽  
A. El-Banna
Keyword(s):  
Plant Regeneration ◽  
Solanum Lycopersicum ◽  
Callus Induction ◽  
Ms Medium ◽  
Regeneration System ◽  
Benzyl Adenine ◽  
Cotyledon Explants ◽  
Tomato Genotypes ◽  
Number Of Shoots

The present study was carried out for developing an efficient in vitro callus induction and plant regeneration system in four different tomato genotypes (Solanum lycopersicum Mill., previous name: Lycopersicon esculentum), Advantage II, Edkawy, Castle Rock and Super Strain B, using hypocotyl and cotyledon explants. The effects of two cytokinins, BA (benzyl adenine) and Kin (kinetin), on callus induction and plant regeneration frequency were investigated when added to MS medium in combination at varying concentrations. All concentrations of the two cytokinins were suitable for callus induction and plant regeneration. The frequency of callus induction and plant regeneration from both cotyledon and hypocotyl explants reached 100% for all tested genotypes. Cotyledons produced a higher average number of shoots per explants than hypocotyls for all the genotypes in the five concentrations of combined cytokinins. The average number of shoots per explant in Super Strain B was found to be the highest (42 and 60 for the hypocotyl and cotyledon explants, respectively). Supplementing MS medium with 1.0 mg L−1 kinetin and 1.0 mg L−1 benzyl adenine was found to be optimum for producing the highest number of shoots per explant from hypocotyls and cotyledons in the tomato genotypes investigated. The proposed medium showed a significant superiority over the reference media.

scholarly journals Trichoderma harzianum como herramienta en el manejo integrado de enfermedades de tomate

2021 ◽  
Author(s):  
◽  
Román Müller
Keyword(s):  
Solanum Lycopersicum ◽  
Trichoderma Harzianum ◽  
Sclerotium Rolfsii ◽  
Trichoderma Spp ◽  
Solanum Lycopersicum L ◽  
Nuevas Tecnologías

En nuestro país como a nivel mundial, el tomate (Solanum lycopersicum L. = Lycopersicun esculetum Mill.), es una de las hortalizas más consumida y de mayor valor económico. Durante su cultivo, se encuentra expuesto a distintas plagas, entre ellas a Sclerotium rolfsii. Para un control eficiente de las enfermedades es conveniente utilizar un manejo integrado de plagas, utilizando distintas técnicas de cultivo tanto clásicas como nuevas tecnologías como el control biológico, que contemplen paralelamente el cuidado del medio ambiente y la producción del cultivo. Trichoderma spp., es uno de los organismos más utilizados para el control biológico de las enfermedades de origen fúngico. El objetivo de este trabajo fue evaluar la eficacia de la cepa de Trichoderma harzianum del IFSC como controlador biológico de Sclerotium rolfsii, ya sea sola o con fungicidas comerciales y analizar su capacidad como promotora de crecimiento del cultivo de tomate platense variedad Luna. Se evaluó el comportamiento de Th del IFSC para controlar a Sclerotium rolfsii in vitro, en cámara de crecimiento y a campo. Como resultado de los ensayos se comprobó que la cepa de Th del IFSC incrementó la germinación en condiciones in vitro e inhibió el desarrollo de S. rolfsii, poniéndose en evidencia el mecanismo de destrucción de los esclerocios. Indujo la promoción del crecimiento de la plántula obteniéndose plantas con mayor sanidad y porte. La cepa Th del IFSC tuvo mayor afinidad con el fungicida Captan incrementando la germinación y el desarrollo de las plántulas en el cultivo de tomate. Se comprobó la factibilidad del uso de Th del IFSC en un planteo de manejo integrado en el cultivo de tomate platense variedad Luna ya sea sola o con un fungicida como Captan.

scholarly journals Вплив похідних піразолу на ріст і розвиток рослин в умовах in vivo та in vitro

2021 ◽  
pp. 108-119
Author(s):  
І.В. Могільнікова ◽  
В.А. Циганкова ◽  
А.О. Гуренко ◽  
В.С. Броварець ◽  
Н.М. Білько ◽  
...  
Keyword(s):  
Solanum Lycopersicum ◽  
Solanum Lycopersicum L

Досліджено вплив синтетичних низькомолекулярних сполук — похідних піразолу — на ріст і розвиток рослин томата (Solanum lycopersicum L.) сорту Money Maker в умовах in vivo та in vitro. Встановлено, що найбільш виражену активність в умовах in vivo виявляє сполука D-pyrazole-3 у концентрації 10–8 М. Вперше досліджено вплив цих сполук на морфогенетичний потенціал експлантів S. lycopersicum в умовах in vitro і показано, що сполуки D-pyrazole-1, D-pyrazole-2 і D-pyrazole-3 ефективно індукують калюсогенез та ризогенез, а також, залежно від концентрації, пряму регенерацію рослин в умовах in vitro. Під дією всіх трьох сполук як у концентрації 10–9 М, так і 10–8 М найефективніше коренеутворення відбувається на пагонових експлантах. Для прямої регенерації рослин із цього типу експлантів найбільш ефективними є сполуки D-pyrazole-1 у концентрації 10–9 М або D-pyrazole-2 у концентрації 10–8 М, які в подальшому можуть бути рекомендовані для використання в біотехнологічних дослідженнях з генетичного вдосконалення S. lycopersicum.

scholarly journals The influence of the nutrient medium composition on the induction of calluso- and morphogenesis of Melissa officinalis L.

2020 ◽  
Author(s):  
O.V. Yakimova ◽  
◽  
N.A. Yegorova ◽  
Keyword(s):  
Callus Induction ◽  
Nutrient Medium ◽  
Culture Medium ◽  
Medium Composition ◽  
Ms Medium ◽  
Explant Type ◽  
Maximum Frequency ◽  
Melissa Officinalis ◽  
Exogenous Factors

The features of the calluso- and morphogenesis induction during the cultivation of tissues and organs of Melissa officinalis depending on endogenous and exogenous factors were revealed. The maximum frequency of callus induction (59.5–92.9 %) was noted on the MS medium with 1.0 mg/l 2.4-D and 0.5 mg/l BAP. The induction of morphogenesis from callus was influenced by the composition of the culture medium, the explant type and cultivar. The maximum frequency of morphogenesis induction (20.0–28.0 % depending on the cultivar) from callus was noted on MS culture medium supplemented with 1.0 mg/L NAA and 0.5 mg/L BAP or 1.0 mg/L NAA and 0.5 mg L TDZ.

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