scholarly journals The influence of the nutrient medium composition on the induction of calluso- and morphogenesis of Melissa officinalis L.

2020 ◽  
Author(s):  
O.V. Yakimova ◽  
◽  
N.A. Yegorova ◽  
Keyword(s):  
Callus Induction ◽  
Nutrient Medium ◽  
Culture Medium ◽  
Medium Composition ◽  
Ms Medium ◽  
Explant Type ◽  
Maximum Frequency ◽  
Melissa Officinalis ◽  
Exogenous Factors

The features of the calluso- and morphogenesis induction during the cultivation of tissues and organs of Melissa officinalis depending on endogenous and exogenous factors were revealed. The maximum frequency of callus induction (59.5–92.9 %) was noted on the MS medium with 1.0 mg/l 2.4-D and 0.5 mg/l BAP. The induction of morphogenesis from callus was influenced by the composition of the culture medium, the explant type and cultivar. The maximum frequency of morphogenesis induction (20.0–28.0 % depending on the cultivar) from callus was noted on MS culture medium supplemented with 1.0 mg/L NAA and 0.5 mg/L BAP or 1.0 mg/L NAA and 0.5 mg L TDZ.

scholarly journals Adjustment of culture media to optimize in vitro rhizogenesis of domestic plums of various origins

2021 ◽  
Vol 34 ◽  
pp. 03001
Author(s):  
Natalia Kovalenko ◽  
Nadezhda Polivara
Keyword(s):  
Nutrient Medium ◽  
Culture Medium ◽  
Root Formation ◽  
Culture Media ◽  
Medium Composition ◽  
Ms Medium ◽  
Growth Substances ◽  
Nutrient Media ◽  
Plum Varieties

This paper presents the results of the adjustment of 18 modifications of culture medium based on MS medium composition – Murashige and Skoog (1962) for in vitro rhizogenesis of 10 varieties of domestic plum. There were used 4 nutrient media with a full amount of macro- and microelements – MS1 with a different combination of phytohormones, 1 medium without hormones – MSc, 13 media – MS2 with half the amount of macronutrients, differing in hormonal composition. It was found that the maximum number of rooted microshoots from 21.2 to 52.2 % was on MS2–8 medium, from 11.2 to 43.1 % on MS2–5. The analysis showed that into the medium increases the percentage of microplants by 12-17 % comparing with the medium MS2–5 and MS2–7 without FA. The distinctiveness of nutrient media by the type of auxin (IAA, NAA, IBA) made it possible to clarify that IBA is the most optimal of the auxins, and the concentration of 1.7 mg/l is borderline for the growth reactions of plum varieties. It was revealed that in vitro root formation depends not only on the compositions of growth substances in the nutrient medium, but also on the genotype of the variety.

scholarly journals Culture medium for mint micropropagation in vitro

2020 ◽  
Author(s):  
N.A. Yegorova ◽  
◽  
M.S. Zagorskaya ◽  
O.V. Yakimova ◽  
◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Culture Medium ◽  
Medium Composition ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Second Stage ◽  
Clonal Micropropagation ◽  
Propagation Technique

The influence of the culture medium composition on the development of explants at the second stage of clonal micropropagation of mint (Mentha canadensis L. K59(4n)) was studied in order to improve the in vitro propagation technique. It was shown that the maximum multiplication rate (11.5) was provided by MS medium supplemented with BAP (1.0 mg/L), IAA (0.5 mg/L) and 2% sucrose.

Production of Isoflavonoids in Callus Cultures of Pueraria candollei var. mirifica

2009 ◽  
Vol 64 (3-4) ◽  
pp. 239-243 ◽  
Author(s):  
Latiporn Udomsuk ◽  
Kanokwan Jarukamjorn ◽  
Hiroyuki Tanaka ◽  
Waraporn Putalun
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Callus Induction ◽  
Culture Medium ◽  
Callus Cultures ◽  
Stem Explants ◽  
Ms Medium ◽  
Shoot Induction ◽  
Pueraria Candollei

Pueraria candollei Wall. ex Benth. var. mirifica (Airy Shaw & Suvat.) Niyomdham was investigated for callus induction using Murashige and Skoog (MS) medium containing different plant growth regulators. After 8 weeks of culture, 66 - 100% of leaf or stem explants formed calli. Calli from stem explants cultured on MS medium supplemented with 0.5 mg/l thidiazuron (TDZ) gave the maximum of shoot induction (16%) and the highest level of total isoflavonoids [(50.39 ± 7.06) mg/g dry wt], which was 7-fold higher than that of the native tuber [(7.04 ± 0.29) mg/g dry wt]. These results suggest that addition of TDZ to the culture medium markedly enhances the production of isoflavonoids in calli induced from stem explants of P. candollei var. mirifica.

scholarly journals INDUKSI KALUS CENGKEH DARI EKSPAN DAUN MENGGUNAKAN 2,4-D SECARA IN VITRO

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Yulianti Rasud ◽  
Zainuddin Basri ◽  
Nirwan Sahiri
Keyword(s):  
Growth Regulators ◽  
Callus Induction ◽  
Callus Formation ◽  
Cell Mass ◽  
Medium Composition ◽  
Ms Medium ◽  
Plant Parts ◽  
Randomized Design ◽  
Completely Randomized Design

ABSTRACT Callus induction is one method of tissue culture which is done by stimulating cell division continuously from certain plant parts such as leaves, roots, stems, and so on by using growth regulators to form cell mass. The cell mass (callus) will then regenerate through organogenesis or embryogenesis to become a new plant. One of the growth regulators used for callus induction is 2,4-D. The aims of this experiments was to evaluate the best concentration of 2,4-D for callus induction of clove leaves. The experiment used Completely Randomized Design with treatment tested was concentrations of 2,4-D, consisted of six levels, namely 0.5 ppm, 1.0 ppm, 1.5 ppm, 2.0 ppm, 2.5 ppm and 3.0 ppm. Results of this experiments indicated that the best medium composition for callus induction was MS medium supplemented with 0.5 ppm 2,4-D.  In the medium composition, the fastest callus formation, namely 6.00 weeks after culture and the percentage of callus formation reached 100% with the color and texture of the resulting callus white and crumb. Keyword : Callus Induction, Clove, 2,4-DABSTRAK Induksi kalus merupakan salah satu metode kultur jaringan yang dilakukan dengan jalan memacu pembelahan sel secara terus menerus dari bagian tanaman tertentu seperti daun, akar, batang, dan sebagainya dengan menggunakan zat pengatur tumbuh hingga terbentuk massa sel. Massa sel (kalus) tersebut selanjutnya akan beregenerasi melalui organogenesis ataupun embriogenesis hingga menjadi tanaman baru. Salah satu zat pengatur tumbuh yang digunakan untuk induksi kalus adalah 2,4-D. Penelitian ini bertujuan menentukan konsentrasi 2,4-D yang lebih baik untuk induksi kalus daun cengkeh.  Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan tiga kali ulangan. Media dasar yang digunakan adalah media MS yang ditambahkan berbagai konsentrasi 2,4-D yaitu 0,50 ppm, 1,5 ppm, 2 ppm, 2,5 ppm, dan 3 ppm. Hasil penelitian menunjukkan bahwa komposisi media yang terbaik untuk induksi kalus daun cengkeh adalah media MS yang ditambahkan 0,5 ppm 2,4-D.  Pada komposisi media tersebut diperoleh saat muncul kalus paling cepat, yaitu rata-rata 6,00 MST dengan persentase pembentukan kalus tertinggi mencapai 100% dengan warna dan tekstur kalus yang dihasilkan putih dan remah. Kata Kunci :  Induksi Kalus, Cengkeh, 2,4-D.

Influence of genotype, explant type, and component of culture medium on in vitro callus induction and shoot organogenesis of tomato (Solanum lycopersicum L.)

2014 ◽  
Vol 41 (6) ◽  
pp. 512-521 ◽  
Author(s):  
M. R. Khaliluev ◽  
L. R. Bogoutdinova ◽  
G. B. Baranova ◽  
E. N. Baranova ◽  
P. N. Kharchenko ◽  
...  
Keyword(s):  
Solanum Lycopersicum ◽  
Callus Induction ◽  
Culture Medium ◽  
Shoot Organogenesis ◽  
Explant Type ◽  
Solanum Lycopersicum L

scholarly journals Peculiarities of Thymus tauricus Klokov et Des.-Shost. clonal micropropagation

2020 ◽  
Author(s):  
A.Sh. Tevfik ◽  
◽  
N.A. Yegorova ◽  
Keyword(s):  
Culture Medium ◽  
Medium Composition ◽  
Ms Medium ◽  
Optimal Composition ◽  
Cultivation Conditions ◽  
Second Stage ◽  
Clonal Micropropagation

The aim of the investigation was to study the influence of cultivation conditions and the culture medium composition on the Thymus tauricus Klokov et Des.-Shost explants morphogenesis at the 1st-2nd stages of clonal micropropagation. The optimal composition of culture medium at the introduction stage is the MS medium with 1.0 mg/l of kinetin and 1.0 mg/l GА3. To obtain a high multiplication index (29.4) at the second stage of micropropagation, it is necessary to cultivate explants in flasks with MS medium supplemented with 1.0 mg/l kinetin.

scholarly journals Study on Callus Induction System of 4 Genotype of Napiergrass (Pennisetum purpureum )

2016 ◽  
Vol 18 (3) ◽  
pp. 131
Author(s):  
Nafiatul Umami ◽  
Ryo Akashi ◽  
Takahiro Gondo ◽  
Genki Ishigaki ◽  
Hidenori Tanaka
Keyword(s):  
Genetic Transformation ◽  
Callus Induction ◽  
Medium Composition ◽  
Pennisetum Purpureum ◽  
Induction Medium ◽  
Ms Medium ◽  
Embryogenic Calli ◽  
Improvement Program ◽  
Shoot Apices ◽  
Best Response

The aim of this study was to produce callus induction potential of 4 napiergrass (Pennisetum purpureum) genotypes (Dwarf Late, Hybrid, Merkeron and Wrukwona). Callus was induced from shoot apices of shoot tillers  on MS media containing 2,4-D and BAP. On the MS medium containing 2 mg L–1 2,4-D and 0.5 mg L–1 BAP all genotypes could produce embryogenic calli, with different rate of growth. The best genotype for producing embryogenic calli was dwarf napiergrass in 60 day culture. These genotypes would be usefull for tissue cultured based research and for napiergrass improvement program, particularly in genetic transformation. Culturing shoot apices on MS medium containing 2 mgL–1 2,4-D and 0.5 mgL–1 BAP was more suitable than on MS medium containing 0.5 mgL–1 2,4-D.  In the subculture with similar medium composition, proliferation occured poorly on dwarf napiergrass, whereas none happened on the three other genotypes. On the hormon-free medium, all genotypes germinated in different rates. This research pointed out that dwarf napiergrass gave the best response toward induction medium. However, its proliferation and regeneration needed to be optimized in order to obtain more obvious data. This genotype would be usefull for tissue culture based research and for napiergrass improvement program, particularly in genetic transformation.

scholarly journals Influence of nutrient medium composition on the morphological characteristics of culture of dorsal root ganglion cells of neonatal piglets

2018 ◽  
Keyword(s):  
Cell Culture ◽  
Dorsal Root Ganglion ◽  
Glial Cells ◽  
Cell Cultures ◽  
Nutrient Medium ◽  
Dorsal Root ◽  
Culture Medium ◽  
Medium Composition ◽  
Satellite Glial Cells ◽  
Neonatal Piglets

Dorsal root ganglion (DRG) is a potential source of neural stem cells because it contains neural crest derived cells that are capable to differentiate into neurons and glial cells. Cell cultures obtained from animals that are close to humans by physiological characteristics can be regarded as an adequate modern model for in vitro studies. In this respect, DRG cell culture obtained from the domestic pig (Sus scrofa domesticus) is a convenient model. The aim of the work was to obtain a primary cell culture of DRG of neonatal piglets and to study its morphological and proliferative properties depending on culture medium composition. The composition of the media prepared on the basis of α-MEM varied depending on the presence of fetal calf serum (FCS) or its modern supplements B-27 and NeuroMax. It is established that morphological differences of primary DRG cell cultures of neonatal pigs depend on the composition of the nutrient medium. When cultured in the presence of 10% FCS, the formation of monolayer which includes satellite glial cells (SGC) and fibroblast-like cells was observed. Small colonies of neurons producing long processes were on the monolayer. When cultured in the presence of NeuroMax and B-27 supplements, the bulk of the cells is not attached, but organized into floating multicellular spheroids (MS). With the passage of culture obtained in the presence of 10% FCS, rapid attachment and proliferation of cells was observed. When MS obtained in the presence of NeuroMax and B-27 were transferred to the medium with 10% FCS, the attachment of MS to the substrate and cell migration were observed. The cells retain the ability to actively proliferate, because the monolayer achieves confluence by 5–7 days of subculture. Regardless of the composition of the primary culture medium, there were 3 morphologically different types of cells in the subcultures: SGC, neuron-like and fibroblast-like cells. The type of cells prevailing in the subculture depends on the composition of the nutrient medium. When MS is transferred from a B-27-containing medium, a significant growth of fibroblast-like cells is observed, whereas when MS is transferred from NeuroMax-containing medium MG and neuron-like cells were abundant.

scholarly journals Effect of Benzyl Adenine Concentration on Callus Induction of Geranium Plants (Pelargonium graveolens L'Her) from Petiole and Leaf Explants

2020 ◽  
Vol 10 (1) ◽  
pp. 20-23
Author(s):  
Moch Faizul Huda ◽  
◽  
Serafinah Indriyani
Keyword(s):  
Callus Induction ◽  
Culture Medium ◽  
Callus Formation ◽  
Leaf Explants ◽  
Ms Medium ◽  
Morphological Parameters ◽  
Benzyl Adenine ◽  
Petiole Explants ◽  
Pelargonium Graveolens ◽  
Light Green

Geranium plant (Pelargonium graveolens L'Her) is one of the geranium oil producing plants that has many benefits. Callus culture is a technique that can be used to plant multiplication and increase production of secondary metabolites. This study aims to determine the effect of the concentration of Benzyl Adenine on the formation of geranium callus from petiole and leaf explants. Callus induction was carried out by culturing petiole and leaf explants on MS medium + 0.1 mg.L-1 NAA + Benzyl Adenine (0; 0.5; 1; 1.5 and 2 mg.L-1). Callus morphological parameters, percentage of callus formation, and time of first callus formation were observed. The formation of geranium callus influenced by the explant type and the concentration of Benzyl Adenine. In the 2nd week, the geranium callus was initiated, light green colored with a compact callus texture. At 4th week, the percentage of callus formation containing NAA 0.1 mg.L-1 of petiole and leaf explants was 20% and 8%, whereas the percentage of callus formation on medium containing 0.1 mg.L-1 NAA combined with 0.5-2 mg.L-1 Benzyl Adenine of petiole and leaf explants was 52-80% and 24-52%. The best percentage of callus formation was found on the culture medium containing 1 mg.L-1 Benzyl Adenine, equaled 80% of petiole explants, and 52% of leaf explants.

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