Dendrobium Hybrid Flower Number and Pedicel Curvature is Influenced by the Application of Gibberellic Acid and Indole-3-acetic Acid

2021 ◽  
Vol 48 (6) ◽  
pp. 740-745
Author(s):  
Muhammad-Asyraf Khairul-Anuar ◽  
Purabi Mazumdar ◽  
Sam Lum ◽  
Jennifer Ann Harikrishna
Molecules ◽  
2017 ◽  
Vol 22 (3) ◽  
pp. 374 ◽  
Author(s):  
Chang Park ◽  
Hyeon Yeo ◽  
Yun Park ◽  
Abubaker Morgan ◽  
Mariadhas Valan Arasu ◽  
...  

HortScience ◽  
2011 ◽  
Vol 46 (7) ◽  
pp. 1010-1014 ◽  
Author(s):  
Todd J. Rounsaville ◽  
Darren H. Touchell ◽  
Thomas G. Ranney ◽  
Frank A. Blazich

Mahonia ‘Soft Caress’ is a unique new cultivar exhibiting a compact form and delicate evergreen leaves. Protocols for micropropagation of M. ‘Soft Caress’ were developed to expedite multiplication and serve as a foundation for future work with other taxa of Mahonia Nutt. Combinations of sucrose at 30 or 45 g·L−1 in conjunction with Gamborg B5 (B5), Quoirin and Lepoivre (QL), and Murashige and Skoog (MS) basal media as well as other selected growth regulator treatments were evaluated as multiplication media. Rooting of microcuttings was conducted in vitro using combinations of indole-3-butyric acid (IBA) at 0, 2, 4, 8, or 16 μM under either light or dark. Quick dip treatments with aqueous solutions of the potassium (K) salt (K-salt) of IBA at 0, 5.2, 10.4, 20.7, or 41.4 μM were tested in a second experiment for ex vitro rooting. Media containing B5 basal salts and vitamins supplemented with sucrose at 30 g·L−1, 5 μM 6-benzylaminopurine, 5 μM kinetin, 0.5 μM indole-3-acetic acid, and 2.5 μM gibberellic acid yielded 2.80 ± 0.14 microshoots with a mean length of 14.76 ± 0.63 mm over a 6-week culture period and was an optimal multiplication media. Light treatment and IBA concentration had a significant effect on rooting percentages. Microcuttings treated with 8 μM IBA and maintained in the dark resulted in the best rooting (70%) and ex vitro establishment.


Weed Science ◽  
1976 ◽  
Vol 24 (4) ◽  
pp. 349-352 ◽  
Author(s):  
K. B. Paul ◽  
J. L. Crayton ◽  
P. K. Biswas

Freshly harvested seeds of Florida pusley (Richardia scabraL.) require light for germination. Gibberellic acid (GA3) successfully replaced the light requirement of both scarified and unscarified seeds, while cyclic 3′,5′-adenosine monophosphate (C-AMP) stimulated the dark germination of scarified seeds only. Cold-stratification reduced seed germinability. The germination inhibition by cold-stratification was reversed by both GA3and KNO3treatments. Indole-3-acetic acid (IAA), kinetin, thiourea, and acetylcholine (ACh), either had no effect or reduced the germination percentages of Florida pusley seeds under all conditions studied.


2020 ◽  
Vol 19 (1) ◽  
pp. 39-47 ◽  
Author(s):  
Sabitri Maharjan ◽  
Laxmi Sen Thakuri ◽  
Bir Bahadur Thapa ◽  
Shreeti Pradhan ◽  
Krishna K. Pant ◽  
...  

 The immature seeds of Dendrobiumchryseum, asympodial epiphytic orchid with yellow flowers, were cultured in vitro, and the resultant protocorms were used as explants for seedling development. Protocorms were cultured on½ M.S. medium fortified with Kinetin (Kn), 6-Benzylaminopurine (BAP), and Gibberellic Acid (GA3) in three concentrations (0.5mg/l, 1.0mg/land 2.0 mg/l) both alone and supplemented with 5% and 10% coconut water (C.W.). The highest number of shootsofD. chryseum developed on ½ - M.S. medium fortified with 2.0mg/lofKn and10% C.W. and the longest shootsdeveloped on ½ M.S. media fortified with 1.0mg/lGA3, and 10% C.W. The shoot derived from protocorms were placed in ½ M.S. medium fortified with three different rooting hormones, Indole -3- acetic acid (IAA), Indole -3-butyric acid (IBA) and α-Naphthalene acetic acid (NAA) in different concentrations alone as well as with each 1.0mg/l hormone combined with 10% C.W. The most effective of these media was ½ M.S. medium fortified with 1.5 mg/l IAA for rooting as well as for the production of longest roots. The present study could be useful for standardizing the protocol for mass propagation of the endangered orchid Dendrobiumchryseum.


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