Karakteristik Lahan untuk Kesesuaian Tanaman Apel (Malus sylvestris Mill.) di Kecamatan Sindang Dataran, Kabupaten Rejang Lebong, Bengkulu

<p><strong>[<em>Land Characteristics for Suitability of Apples</em> (<em>Malus sylvestris</em> Mill.) <em>in Sindang Dataran District, Rejang Lebong Regency, Bengkulu</em>]</strong></p><p><strong><br /></strong></p><p>Penilaian kesesuaian lahan merupakan tahap pertama dan penting dalam usaha pengembangan suatu komoditas pertanian. Kecamatan Sindang Dataran, Kabupaten Rejang Lebong merupakan salah satu lokasi yang direncanakan menjadi daerah pengembangan apel dengan temperatur udara mencapai 23oC dan ketinggian tempat bervariasi antara 785 – 1.129 lebih m dpl. dengan kondisi tanah yang cukup subur. Penelitian bertujuan untuk menentukan kelas kesesuaian lahan untuk tanaman apel. Penelitian dilakukan dengan metode survey dan observasi. Parameter yang diamati meliputi karakteristik lahan dan syarat tumbuh tanaman apel. Evaluasi kesesuaian lahan yang digunakan ialah kesesuaian lahan kualitatif, yaitu yang hanya didasarkan kondisi fisik lahan. Metode evaluasi kesesuaian lahan dilakukan dengan mengikuti prosedur dari FAO (1976), yaitu evaluasi kesesuaian lahan ini dilakukan dengan cara mencocokkan (matching) data antara karakteristik lahan dengan persyaratan tumbuh tanaman apel dan hasilnya didasarkan pada nilai terkecil (hukum minimum) sebagai keputusan kesesuaian lahan. Hasil penelitian menunjukkan bahwa kesesuaian lahan tanaman apel yang berada di Kecamatan Sindang Dataran, Kabupaten Rejang Lebong, dari faktor iklim termasuk S2 (cukup sesuai), sementara dari faktor ketersediaan hara termasuk S3 (sesuai marjinal). Hal yang menjadi faktor pembatas adalah rendahnya P tersedia, tetapi dapat diatasi dengan pemupukan sehingga dapat naik kelas menjadi S2. Melalui usaha pemupukan P maka Kecamatan Sindang Dataran memiliki potensi sebagai daerah pengembangan tanaman apel yang cukup sesuai.</p><p><strong>Keywords</strong></p><p>Karakteristik lahan; Kesesuaian lahan untuk tanaman apel; Malus sylvertris Mill</p><p><strong>Abstract</strong></p><p>Land suitability assessment is the first and important stage in the development of an agricultural commodity. Sindang Dataran District, Rejang Lebong Regency is one of the locations that is planned to be an apple development area with air temperatures reaching 23oC and altitude varying between 785–1,129 meters above sea level with fairly fertile soil conditions. The aim of the study was to determine the land suitability class for apple plants. The research was conducted using survey and observation methods. Parameters observed included land characteristics and growing conditions for apple plants. Land suitability evaluation used qualitative land suitability, which is only based on the physical condition of the land. The land suitability evaluation method is carried out by following the procedure from FAO (1976), namely the evaluation of land suitability is carried out by matching data between land characteristics and the requirements for growing apples and the results are based on the smallest value (minimum law) as a land suitability decision. The results showed that the suitability of the land for apple crops in Sindang Dataran District, Rejang Lebong Regency, from climatic factors including S2 (quite suitable), while from nutrient availability factors including S3 (marginal according to). The limiting factor is the low available P, but it can be overcome by fertilization so that it can be promoted to S2. Through P fertilization efforts, Sindang Dataran District has the potential as a suitable apple crop development area.</p>

Impact of Foliar-Applied Dormancy-Breaking Chemicals on Budburst and Metabolic Changes in Chemical Constituents of Leaves and Fruits of Malus sylvestris "Ein Shamer"

This study was carried out during the two successive seasons of 2016 and 2017 to investigate the impact of dormex, dormex plus mineral oil, dormex plus potassium nitrate, dormex plus calcium nitrate and dormex plus thiourea on bud break, growth, yield and some chemical constituents of Malus sylvestris "Ein Shamer". The trees were grown in loamy sand soil, and sprayed with six treatments (dormex (4%), (dormex (2%) plus mineral oil (2.5%),(dormex (2%) plus potassium nitrate (4%),(dormex (2%) plus calcium nitrate (4%) and (dormex (2%) plus thiourea (1%) and control. Generally, it was found that all studied growth parameters , date of flower bud break, percentage of bud break, fruit-setting ,fruit weight, fruit size, fruit number/tree, yield/tree (kg) and some chemical constituents of leaves (total chlorophyll ,total carbohydrates, total protein, nitrogen, phosphorous and potassium contents) and some chemical constituents of fruits (total soluble solids(T.S.S.), T.S.S/ acid ratio, vitamin C, water content %, total free amino acids, total carbohydrates, total sugars and reducing sugars) were increased with the application of the different treatments. The best results were obtained from the treatments of dormex at 4% followed by (dormex (2%) plus mineral oil (2.5%). On the contrary, the same treatments decreased total acidity and total phenols in fruits as compared to the control. It could be recommended to use dormex at 4% and (dormex (2%) plus mineral oil (2.5%) for improving bud break, growth, yield and chemical constituents of apple trees or fruits.

Advanced genebank management of genetic resources of European wild apple, Malus sylvestris, using genome-wide SNP array data

The Netherlands’ field genebank collection of European wild apple (Malus sylvestris), consisting of 115 accessions, was studied in order to determine whether duplicates and mistakes had been introduced, and to develop a strategy to optimize the planting design of the collection as a seed orchard. We used the apple 20K Infinium single nucleotide polymorphism (SNP) array, developed in M. domestica, for the first time for genotyping in M. sylvestris. We could readily detect the clonal copies and unexpected duplicates. Thirty-two M. sylvestris accessions (29%) showed a close genetic relationship (parent-child, full-sib, or half-sib) to another accession, which reflects the small effective population size of the in situ populations. Traces of introgression from M. domestica were only found in 7 individuals. This indicates that pollination preferentially took place among the M. sylvestris trees. We conclude that the collection can be considered as mainly pure M. sylvestris accessions. The results imply that it should be managed as one unit when used for seed production. A bias in allele frequencies in the seeds may be prevented by not harvesting all accessions with a close genetic relationship to the others in the seed orchard. We discuss the value of using the SNP array to elaborate the M. sylvestris genetic resources more in depth, including for phasing the markers in a subset of the accessions, as a first step towards genetic resources management at the level of haplotypes.

Evaluation of ElecTIS bioreactor for the micropropagation of Malus sylvestris (L.) Mill., an important autochthonous species of Albania

Malus sylvestris (L.) Mill., an economically-important fruit tree, is native to Albania and in many parts of Europe. It is cultivated as an ornamental tree, while its fruits are collected for food and a source of antioxidant substances. It is included in The IUCN Red List of Threatened Species. For these reasons, it is very important to optimise a micropropagation protocol, in order to obtain great numbers of clonal plantlets for ex situ conservation and production purposes. A liquid culture in a temporary immersion system (TIS) is a recently-proposed system for large-scale in vitro plant propagation. In this study, lateral buds of M. sylvestris were inoculated in MS medium with BAP (1 mg/L) and NAA (0.1 mg/L). In order to avoid oxidative stress, different antioxidants were previously tested with the culture in a gelled medium, and the combination of ascorbic acid and citric acid (both at 100 mg/L) was selected for the following culture in TIS. Stabilised explants were then cultivated in ElecTIS, an innovative TIS bioreactor, and in a semisolid medium, after which the two culture systems were evaluated. Overall, the ElecTIS showed to be more effective for all the tested parameters.

Hybridisation of Malus sylvestris (L.) Mill. with Malus × domestica Borkh. and Implications for the Production of Forest Reproductive Material

This study focuses on the morphological and genetic characteristics of European crab apple (Malus sylvestris (L.) Mill.) and the occurrence of hybrids in its populations. We analyzed a total of 107 putative European crab apple trees in Slovenia: 92 from nine natural populations, five from a seed stand and 10 from a stand of unnatural origin. We also included 18 domesticated apple trees (Malus × domestica Borkh.) and two Japanese flowering crab apple trees (Malusfloribunda van Houtte) as outliers. The trees were classified into groups of European crab apples, hybrids and domesticated apples according to their morphological and genetic characteristics. Classification based on morphological traits produced different results (58.75% European crab apple, 37.11% hybrids and 4.14% domesticated apple) compared to those based on genetic analysis (70.10% European crab apple, 21.64% hybrids and 8.26% domesticated apple). When genetic and morphological characteristics were combined, only 40.20% of the trees were classified as European crab apple, and an additional group of feral cultivars of domesticated apples (6.18%) was identified. The analysis revealed that hybridization with domesticated apple is taking place in all studied natural European crab apple populations; however, hybrids and feral cultivars only occur to a limited extent. When introducing European crab apple into forests in the future, only genetically verified forest reproductive material obtained exclusively from suitable seed stands should be used.

Genetic structure of Malus sylvestris and potential link with preference/performance by the rosy apple aphid pest Dysaphis plantaginea

The European crabappleMalus sylvestris, a crop wild relative ofMalus domestica, is a major contributor to the cultivated apple genome and represents a potential source of interesting alleles or genes, particularly pest resistance traits. An original approach was used to explore the trophic interaction betweenM. sylvestrispopulations and its pest, the rosy apple aphid (Dysaphis plantaginea). Using 13 microsatellite markers, population genetic structure and level of crop-to-wild introgressions were inferred betweenM. sylvestrisseedlings from three sites in Europe (Denmark, France, Romania), andM. domesticaseedlings. Genetically characterized plants were also used to analyze aphid feeding behavior and fitness parameters. First, aphids submitted to two genetically closeM. sylvestrispopulations (the Danish and French) exhibited similar behavioral parameters, suggesting similar patterns of resistance in these host plants. Second, the RomanianM. sylvestrispopulation was most closely genetically related toM. domestica. Although the two plant genetic backgrounds were significantly differentiated, they showed comparable levels of sensitivity toD. plantagineainfestation. Third, aphid fitness parameters were not significantly impacted by the host plant’s genetic background. Finally, crop-to-wild introgression seemed to significantly drive resistance toD. plantagineaindependent of host plant population genetic structure, with hybrids being less suitable hosts.

Metabolic Changes in Phenolic Compounds in Buds during and after Dormancy Releasing in early and late (Malus sylvestris, Mill) Apple Varieties as Effected by Chilling Requirements

In order to study the relation between seasonal changes in phenolic compounds and flower opining date according to chilling requirements. The early and late-opining apple varieties, Barkhar, Local and Strakhan (Malussylvestris) were used. This study investigated variations in chilling requirements, bud burst and development in early and late varieties of apple trees. Results showed less bud burst in late varieties than in early ones. In the former, there were increased in phenolic compounds (conjugated and total phenols) at budburst in all varieties. As dormancy begins, free phenols are increased, coinciding with a reduction in the levels of conjugated phenols. Consequently, as dormancy breaks, these free phenols are conjugate with organic constituents, and a decrease in the concentrations of free phenols occurs, in order to reduce inhibitory effect on growth. We conclude that late varieties (Strakhan) are less economical in manufacturing new growth, as indicated by less bud vigor at budburst than early varieties (Barkhar and local) and show a marked differential phenols compound pattern throughout bud development compared to early varieties.