Influence of Methyl Jasmonate on Production of Ecdysteroids from Hairy Roots of Silene linicola C.C. Gmelin

Valtrate is a pharmacologically active epoxyiridoid ester found in the roots and rhizomes of Valeriana jatamansi Jones. The plant produces only small amounts of this metabolite naturally, and so induction of hairy roots as well as elicitation can be useful to increase its commercial production. In this study, strain R1601 of Agrobacterium rhizogenes was used to induce hairy roots in V. jatamansi, and stable hairy root cultures of V. jatamansi were established successfully. The influence of three exogenous elicitors including methyl jasmonate (MJ), jasmonic acid (JA) and salicylic acid (SA) on valtrate production in the hairy root cultures of V. jatamansi was also investigated, and the 25-day-old hairy root cultures were treated with different concentrations of the elicitors at exposure time of 7 days. This present study showed that MJ (100 mg/L) highly promoted valtrate production at 7 days after elicitation, to a level of 3.63 times higher than that of non-elicited control. SA did not significantly increase the production of valtrate. This is the first-time study to assess the elicitation of hairy root cultures to promote valtrate biosynthesis in V. jatamansi and the resulting experiments demonstrated that MJ was indeed a potent inducer of valtrate biosynthesis.

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Production of Sesquiterpene-Type Phytoalexins by Hairy Roots of Hyoscyamus albus Co-treated with Cupper Sulfate and Methyl Jasmonate

Chemical and Pharmaceutical Bulletin ◽

10.1248/cpb.58.934 ◽

2010 ◽

Vol 58 (7) ◽

pp. 934-938 ◽

Cited By ~ 16

Author(s):

Morihiro Kawauchi ◽

Toshihide Arima ◽

Osamu Shirota ◽

Setsuko Sekita ◽

Takahisa Nakane ◽

...

Keyword(s):

Methyl Jasmonate ◽

Hairy Roots ◽

Hyoscyamus Albus

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Effects of methyl jasmonate on the growth and triterpenoid production of diploid and tetraploid Centella asiatica (L.) Urb. hairy root cultures

Scientific Reports ◽

10.1038/s41598-019-54460-z ◽

2019 ◽

Vol 9 (1) ◽

Author(s):

Khoa Van Nguyen ◽

Benyakan Pongkitwitoon ◽

Thanika Pathomwichaiwat ◽

Unchera Viboonjun ◽

Sompop Prathanturarug

Keyword(s):

Methyl Jasmonate ◽

Hairy Root ◽

Hairy Roots ◽

Centella Asiatica ◽

Root Culture ◽

Hairy Root Culture ◽

Asiatic Acid ◽

Hairy Root Cultures ◽

Root Cultures ◽

Meja Treatment

AbstractIn this study, the effects of methyl jasmonate (MeJA) on the phytomass and triterpenoid production of diploid and tetraploid Centella asiatica hairy roots were investigated. Hairy root cultures were obtained from diploid and induced tetraploid plants of C. asiatica infected by Agrobacterium rhizogenes strain ATCC 43057. MeJA triggered triterpenoid production in both ploidy hairy roots, whereas triterpenoids were not produced in the untreated hairy roots. Among the treatments, the 50 µM MeJA treatment yielded the maximum triterpenoid production in diploid hairy roots of 27.25 ± 0.27 µg/mg Dry weight (DW) total triterpenoid at day 21. For the tetraploid hairy root cultures, the 28th-day hairy root culture produced a maximum amount of triterpenoids of 16.29 ± 6.32 µg/mg DW in response to the 50 µM MeJA treatment, whereas the 100 µM MeJA treatment produced a similar triterpenoid amount (16.31 ± 9.24 µg/mg DW) at day 14. Moreover, in response to 50 µM MeJA, we obtained different ratios of aglycone to glycoside, i.e., 1:7 and 1:2, between the diploid and tetraploid hairy root cultures. Asiaticoside was the dominant phytochemical, followed by asiatic acid and madecassic acid. This study provides valuable information for producing triterpenoids for C. asiatica commercial products and preparations by using hairy root cultures.

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Differential Secretion and Accumulation of Terpene Indole Alkaloids in Hairy Roots of Catharanthus roseus Treated with Methyl Jasmonate

Molecular Biotechnology ◽

10.1007/s12033-008-9111-2 ◽

2008 ◽

Vol 41 (3) ◽

pp. 278-285 ◽

Cited By ~ 42

Author(s):

Eliel Ruiz-May ◽

Rosa M. Galaz-Ávalos ◽

Víctor M. Loyola-Vargas

Keyword(s):

Methyl Jasmonate ◽

Hairy Roots ◽

Catharanthus Roseus ◽

Indole Alkaloids ◽

Terpene Indole Alkaloids

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PEG and ABA trigger methyl jasmonate accumulation to induce the MEP pathway and increase tanshinone production in Salvia miltiorrhiza hairy roots

Physiologia Plantarum ◽

10.1111/j.1399-3054.2012.01603.x ◽

2012 ◽

Vol 146 (2) ◽

pp. 173-183 ◽

Cited By ~ 53

Author(s):

Dongfeng Yang ◽

Pengda Ma ◽

Xiao Liang ◽

Zheng Wei ◽

Zongsuo Liang ◽

...

Keyword(s):

Methyl Jasmonate ◽

Hairy Roots ◽

Salvia Miltiorrhiza ◽

Mep Pathway

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Stimulation of Rg3 ginsenoside biosynthesis in ginseng hairy roots elicited by methyl jasmonate

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/s11240-012-0218-6 ◽

2012 ◽

Vol 112 (1) ◽

pp. 87-93 ◽

Cited By ~ 24

Author(s):

Ok Tae Kim ◽

Nam Hee Yoo ◽

Gum Soog Kim ◽

Young Chang Kim ◽

Kyong Hwan Bang ◽

...

Keyword(s):

Methyl Jasmonate ◽

Hairy Roots ◽

Ginsenoside Biosynthesis ◽

Stimulation Of

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Cerium-Promoted Ginsenosides Accumulation by Regulating Endogenous Methyl Jasmonate Biosynthesis in Hairy Roots of Panax ginseng

Molecules ◽

10.3390/molecules26185623 ◽

2021 ◽

Vol 26 (18) ◽

pp. 5623

Author(s):

Ru Zhang ◽

Shiquan Tan ◽

Bianling Zhang ◽

Pengcheng Hu ◽

Ling Li

Keyword(s):

Methyl Jasmonate ◽

Hairy Roots ◽

Signal Pathways ◽

Ros Production ◽

Oxygen Species ◽

Direct Stimulation ◽

Key Enzyme ◽

Mda Content ◽

Ginsenoside Content ◽

Stimulation Of

Among rare earth elements, cerium has the unique ability of regulating the growth of plant cells and the biosynthesis of metabolites at different stages of plant development. The signal pathways of Ce3+-mediated ginsenosides biosynthesis in ginseng hairy roots were investigated. At a low concentration, Ce3+ improved the elongation and biomass of hairy roots. The Ce3+-induced accumulation of ginsenosides showed a high correlation with the reactive oxygen species (ROS), as well as the biosynthesis of endogenous methyl jasmonate (MeJA) and ginsenoside key enzyme genes (PgSS, PgSE and PgDDS). At a Ce3+ concentration of 20 mg L−1, the total ginsenoside content was 1.7-fold, and the total ginsenosides yield was 2.7-fold that of the control. Malondialdehyde (MDA) content and the ROS production rate were significantly higher than those of the control. The activity of superoxide dismutase (SOD) was significantly activated within the Ce3+ concentration range of 10 to 30 mg L−1. The activity of catalase (CAT) and peroxidase (POD) strengthened with the increasing concentration of Ce3+ in the range of 20–40 mg L−1. The Ce3+ exposure induced transient production of superoxide anion (O2•−) and hydrogen peroxide (H2O2). Together with the increase in the intracellular MeJA level and enzyme activity for lipoxygenase (LOX), there was an increase in the gene expression level of MeJA biosynthesis including PgLOX, PgAOS and PgJMT. Our results also revealed that Ce3+ did not directly influence PgSS, PgSE and PgDDS activity. We speculated that Ce3+-induced ROS production could enhance the accumulation of ginsenosides in ginseng hairy roots via the direct stimulation of enzyme genes for MeJA biosynthesis. This study demonstrates a potential approach for understanding and improving ginsenoside biosynthesis that is regulated by Ce3+-mediated signal transduction.

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