Differential Secretion and Accumulation of Terpene Indole Alkaloids in Hairy Roots of Catharanthus roseus Treated with Methyl Jasmonate

2008 ◽  
Vol 41 (3) ◽  
pp. 278-285 ◽  
Author(s):  
Eliel Ruiz-May ◽  
Rosa M. Galaz-Ávalos ◽  
Víctor M. Loyola-Vargas
Keyword(s):  
Methyl Jasmonate ◽  
Hairy Roots ◽  
Catharanthus Roseus ◽  
Indole Alkaloids ◽  
Terpene Indole Alkaloids

scholarly journals Role of Different Transporting Systems in the Secretion of Alkaloids by Hairy Roots of Catharanthus roseus (L) G. Don

2017 ◽  
Vol 59 (2) ◽  
Author(s):  
Víctor Manuel Loyola-Vargas
Keyword(s):  
Acetylsalicylic Acid ◽  
Hairy Roots ◽  
Catharanthus Roseus ◽  
Abc Transporters ◽  
Indole Alkaloids ◽  
Hairy Root Cultures ◽  
Biosynthetic Pathways ◽  
Root Cultures ◽  
Transport Inhibitors

<p>Knowledge on the biosynthetic pathways of the monoterpene alkaloids is enormous, but little is known about their mechanism of transporting system from the plant cell. There is not concrete evidence confirming the role of ABC transporters in the secretion of monoterpene indole alkaloids (MIAs) in <em>Catharanthus roseus</em>. Therefore, in order to determine the role of different transporting systems involved in the MIAs translocation, we employed a pharmacological approach by using transport inhibitors such as, KCN, Na3VO4, quinidine and glibenclamide in hairy root cultures of <em>C. roseus</em>. It was found that the accumulation of ATP drastically decreased in the presence of KCN or 100 μM acetylsalicylic acid (ASA)/100 μM KCN. The treatment with the inhibitors KCN and glibenclamide in the presence of ASA significantly increased the ajmalicine secretion compared to the control. The secretion of serpentine was undetected during the first 24 h in all the samples. Treatment with the inhibitors quinidine and glibenclamide provoked a significant reduction of serpentine secretion in the hairy roots compared to the control. Based on our results, we found evidence that ABC transporters might participate in the secretion of MIAs by <em>C. roseus </em>hairy roots.</p>

scholarly journals Methyl Jasmonate Induces ATP Biosynthesis Deficiency and Accumulation of Proteins Related to Secondary Metabolism in Catharanthus roseus (L.) G. Hairy Roots

2011 ◽  
Vol 52 (8) ◽  
pp. 1401-1421 ◽  
Author(s):  
E. Ruiz-May ◽  
C. De-la-Pena ◽  
R. M. Galaz-Avalos ◽  
Z. Lei ◽  
B. S. Watson ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Secondary Metabolism ◽  
Hairy Roots ◽  
Catharanthus Roseus

scholarly journals In vitro growth and content of vincristine and vinblastine of Catharanthus roseus L. hairy roots in response to precursors and elicitors

2022 ◽  
Vol 9 (1) ◽  
pp. 21-28
Author(s):  
Phuong Thi Bach Vu ◽  
Dai Minh Cao ◽  
Anh Lan Bui ◽  
Nhut Nhu Nguyen ◽  
Le Van Bui ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Cancer Treatment ◽  
Methyl Jasmonate ◽  
Agrobacterium Rhizogenes ◽  
Hairy Roots ◽  
Catharanthus Roseus ◽  
In Vitro Growth ◽  
First Time

Catharanthus roseus L. is a medicinal plant that produces numerous indole terpenoid alkaloids, including vincristine and vinblastine, which are used for cancer treatment. The effect of specified precursors (L-phenylalanine, L-tyrosine) and elicitors (chitosan, methyl jasmonate) on C. roseus hairy roots (CHR) growth has been examined in order to increase the content of vincristine and vinblastine. Our results showed that CHR generated by an Agrobacterium rhizogenes strain isolated in Vietnam was capable of producing both vincristine and vinblastine when subjected to precursors, but only vinblastine when exposed to elicitors. However, both precursors and elicitors were evaluated to have an effect on increasing the accumulation of TIAs in CHR. In particular, the use of elicitors required more time to find the appropriate induction conditions, while the use of precursors gave outstanding efficiency in the treatment with 1 µM phenylalanine. The greatest yields of vincristine (51.99 µg g-1 DW) and vinblastine (699.92 µg g-1 DW) were obtained in the 7th week (with 0.306 g DW biomass). This result is the first time we might boost the levels of vincristine and vinblastine in our CHR clone generated by the Vietnam strain of A. rhizogenes.

Regulation of the terpene moiety biosynthesis of Catharanthus roseus terpene indole alkaloids

2007 ◽  
Vol 6 (2-3) ◽  
pp. 341-351 ◽  
Author(s):  
Sabah Hedhili ◽  
Vincent Courdavault ◽  
Nathalie Giglioli-Guivarc’h ◽  
Pascal Gantet
Keyword(s):  
Catharanthus Roseus ◽  
Indole Alkaloids ◽  
Terpene Indole Alkaloids

scholarly journals Preliminary results of indole alkaloids production in different roots of Catharanthus roseus cultured in vitro

2014 ◽  
Vol 70 (4) ◽  
pp. 261-265 ◽  
Author(s):  
Agnieszka Pietrosiuk ◽  
Mirosława Furmanowa
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Hairy Roots ◽  
Catharanthus Roseus ◽  
Indole Alkaloids ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Preliminary Results ◽  
Intact Plants

Six groups of untransformed and hairy root cultures of <em>Catharunthus roseus</em> (L.) G. Don were established. <em>Agrobacterium rhizogenes</em> strains: ATCC 15834, LBA 9403, and TR 105 were used for infection of the 3-week old rooted plantlets of <em>C. roseus</em>. The highest contents of examined indole alkaloids were found in: roots of intact plants - yohimbine and serpentine; in hairy roots - catharanthine. Vinblastine and ajmalicine were detected in untransformed roots of plants regenerated in vitro, and transferred to the soil for 5 months.

scholarly journals Erratum: Production of Sesquiterpene-Type Phytoalexins by Hairy Roots of Hyoscyamus albus Co-treated with Cupper Sulfate and Methyl Jasmonate [Chem. Pharm. Bull. 58(7): 934-938 (2010)]

2010 ◽  
Vol 58 (9) ◽  
pp. 1281
Author(s):  
Moriyuki Kawauchi ◽  
Toshihide Arima ◽  
Osamu Shirota ◽  
Setsuko Sekita ◽  
Takahisa Nakane ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Hairy Roots ◽  
Hyoscyamus Albus
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