Methylation of Histone H3 by Lysine 4 in Neurons of the Mushroom Bodies of the Honeybee Brain during Memory Formation

2020 ◽  
Vol 14 (4) ◽  
pp. 270-274
Author(s):  
T. G. Zachepilo ◽  
N. G. Lopatina
Keyword(s):  
Histone H3 ◽  
Memory Formation ◽  
Mushroom Bodies ◽  
Honeybee Brain

Differential histone acetylation in the Amygdala leads to fear memory consolidation and extinction

2015 ◽  
Vol 1 (1) ◽  
Author(s):  
Vandana Ranjan ◽  
Sanjay Singh ◽  
Sarfraj Ahmad Siddiqui ◽  
M Y Khan ◽  
Anand Prakash
Keyword(s):  
Histone Acetylation ◽  
Memory Consolidation ◽  
Histone H3 ◽  
Fear Memory ◽  
Memory Formation ◽  
Acetylation Level ◽  
Creb Activation ◽  
Conditioning Model ◽  
Histone H3 Acetylation ◽  
H3 Acetylation

<p>In the present study, a fear-conditioning model in rats was used to gauge the changes in the histone acetylation level in the<br />different nuclei of amygdala during fear memory consolidation and its extinction. It was found by immunohistochemical<br />examination of Amygdala that during the fear memory consolidation histone H3 acetylation level was significantly<br />increased in the Central amygdala (CeA), the output of the fear circuitry, as compared to the unconditioned group and<br />subsequently, when this fear memory was extinguished during fear extinction, the histone H3 acetylation levels decreased<br />significantly as compared to the conditioned group. However, in another nuclei of the amygdala, the intercalated cells<br />(ITCs) the Acetyl H3 levels increased during extinction and but not in the conditioned group as compared to the<br />unconditioned group. The p-ERK and p-CREB levels also significantly varied in the different nuclei of amygdala<br />between the two groups and showed correlation with the Histone acetylation changes observed in these groups. In<br />conclusion the present study points out that the memory formation, during fear memory consolidation and its extinction,<br />may be dependent on differential neuronal activity under epigenetic control through acetylation at k-9 residue of histone<br />H3, in different regions of the amygdala as evident by the p-ERK and p-CREB activation, which are the markers for<br />activity of neurons and memory formation.</p>

scholarly journals Identification of proteins whose expression is up- or down-regulated in the mushroom bodies in the honeybee brain using proteomics

FEBS Letters ◽  
2006 ◽  
Vol 581 (1) ◽  
pp. 97-101 ◽  
Author(s):  
Yuko Uno ◽  
Tomoko Fujiyuki ◽  
Mizue Morioka ◽  
Hideaki Takeuchi ◽  
Takeo Kubo
Keyword(s):  
Mushroom Bodies ◽  
Honeybee Brain

scholarly journals Parallel mechanisms of visual memory formation across distinct regions of the honey bee brain

2021 ◽  
Author(s):  
Arián Avalos ◽  
Ian M. Traniello ◽  
Eddie Pérez Claudio ◽  
Tugrul Giray
Keyword(s):  
Honey Bee ◽  
Behavioral Ecology ◽  
Visual Memory ◽  
Visual Learning ◽  
Memory Formation ◽  
Higher Order ◽  
Sensory Transduction ◽  
Floral Resources ◽  
Mushroom Bodies ◽  
Optic Lobes

Visual learning is vital to the behavioral ecology of the Western honey bee (Apis mellifera). Honey bee workers forage for floral resources, a behavior that requires the learning and long-term memory of visual landmarks, but how these memories are mapped to the brain remains poorly understood. To address this gap in our understanding, we collected bees that successfully learned visual associations in a conditioned aversion paradigm and compared gene expression correlates of memory formation in the mushroom bodies, a higher-order sensory integration center classically thought to contribute to learning, as well as the optic lobes, the primary visual neuropil responsible for sensory transduction of visual information. We quantitated expression of CREB and CaMKii, two classical genetic markers of learning and fen-1, a gene specifically associated with punishment learning in vertebrates. As expected, we report substantial involvement of the mushroom bodies for all three markers but additionally demonstrate the involvement of the optic lobes across a similar time course. Our findings imply the molecular involvement of a sensory neuropil during visual associative learning parallel to a higher-order brain region, furthering our understanding of how a tiny brain processes environmental signals.

scholarly journals Postsynaptic plasticity of cholinergic synapses underlies the induction and expression of appetitive memories in Drosophila

2021 ◽  
Author(s):  
Carlotta Pribbenow ◽  
Yi-chun Chen ◽  
Michael-Marcel Heim ◽  
Desiree Laber ◽  
Silas Reubold ◽  
...  
Keyword(s):  
Synaptic Plasticity ◽  
Memory Formation ◽  
Memory Storage ◽  
Mushroom Bodies ◽  
Nachr Subunit ◽  
Nicotinic Acetylcholine ◽  
Cholinergic Synapses ◽  
Presynaptic Plasticity ◽  
Output Neurons

In vertebrates, memory-relevant synaptic plasticity involves postsynaptic rearrangements of glutamate receptors. In contrast, previous work indicates that Drosophila and other invertebrates store memories using presynaptic plasticity of cholinergic synapses. Here, we provide evidence for postsynaptic plasticity at cholinergic output synapses from the Drosophila mushroom bodies (MBs). We find that the nicotinic acetylcholine receptor (nAChR) subunit α5 is required within specific MB output neurons (MBONs) for appetitive memory induction, but is dispensable for aversive memories. In addition, nAChR α2 subunits mediate memory expression downstream of α5 and the postsynaptic scaffold protein Dlg. We show that postsynaptic plasticity traces can be induced independently of the presynapse, and that in vivo dynamics of α2 nAChR subunits are changed both in the context of associative and non-associative memory formation, underlying different plasticity rules. Therefore, regardless of neurotransmitter identity, key principles of postsynaptic plasticity support memory storage across phyla.

scholarly journals A kinase-dependent feedforward loop affects CREBB stability and long term memory formation

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Pei-Tseng Lee ◽  
Guang Lin ◽  
Wen-Wen Lin ◽  
Fengqiu Diao ◽  
Benjamin H White ◽  
...  
Keyword(s):  
Aversive Conditioning ◽  
Memory Deficits ◽  
Memory Formation ◽  
Mushroom Bodies ◽  
Limiting Factor ◽  
Long Term Memory ◽  
Term Memory ◽  
Feedforward Loop ◽  
Genes Encoding

In Drosophila, long-term memory (LTM) requires the cAMP-dependent transcription factor CREBB, expressed in the mushroom bodies (MB) and phosphorylated by PKA. To identify other kinases required for memory formation, we integrated Trojan exons encoding T2A-GAL4 into genes encoding putative kinases and selected for genes expressed in MB. These lines were screened for learning/memory deficits using UAS-RNAi knockdown based on an olfactory aversive conditioning assay. We identified a novel, conserved kinase, Meng-Po (MP, CG11221, SBK1 in human), the loss of which severely affects 3 hr memory and 24 hr LTM, but not learning. Remarkably, memory is lost upon removal of the MP protein in adult MB but restored upon its reintroduction. Overexpression of MP in MB significantly increases LTM in wild-type flies showing that MP is a limiting factor for LTM. We show that PKA phosphorylates MP and that both proteins synergize in a feedforward loop to control CREBB levels and LTM. key words: Drosophila, Mushroom bodies, SBK1, deGradFP, T2A-GAL4, MiMIC

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