The Determination of Cytostatic Activity on a 3D Spheroids-Based Model in Comparison with Conventional Monolayer Culture

2021 ◽  
Vol 15 (6) ◽  
pp. 522-531
Author(s):  
E. V. Koudan ◽  
S. P. Kudan ◽  
S. Sh. Karshieva ◽  
Yu.D. Khesuani ◽  
V. A. Mironov ◽  
...  
Keyword(s):  
Monolayer Culture ◽  
Cytostatic Activity ◽  
3D Spheroids

scholarly journals The Influence of Antitumor Unsymmetrical Bisacridines on 3D Cancer Spheroids Growth and Viability

Molecules ◽  
2021 ◽  
Vol 26 (20) ◽  
pp. 6262
Author(s):  
Jolanta Kulesza ◽  
Monika Pawłowska ◽  
Ewa Augustin
Keyword(s):  
Monolayer Culture ◽  
3D Culture ◽  
Spheroid Culture ◽  
Promising Tool ◽  
Antitumor Compounds ◽  
High Cytotoxicity ◽  
Cancer Spheroids ◽  
2D And 3D ◽  
Hct116 Cells ◽  
3D Spheroids

The culture of 3D spheroids is a promising tool in drug development and testing. Recently, we synthesized a new group of compounds, unsymmetrical bisacridines (UAs), which exhibit high cytotoxicity against various human cell lines and antitumor potency against several xenografts. Here, we describe the ability of four UAs—C-2028, C-2041, C-2045, and C-2053—to influence the growth of HCT116 and H460 spheres and the viability of HCT116 cells in 3D culture compared with that in 2D standard monolayer culture. Spheroids were generated using ultra-low-attachment plates. The morphology and diameters of the obtained spheroids and those treated with UAs were observed and measured under the microscope. The viability of cells exposed to UAs at different concentrations and for different incubation times in 2D and 3D cultures was assessed using 7-AAD staining. All UAs managed to significantly inhibit the growth of HCT116 and H460 spheroids. C-2045 and C-2053 caused the death of the largest population of HCT116 spheroid cells. Although C-2041 seemed to be the most effective in the 2D monolayer experiments, in 3D conditions, it turned out to be the weakest compound. The 3D spheroid culture seems to be a suitable method to examine the efficiency of new antitumor compounds, such as unsymmetrical bisacridines.

scholarly journals Comparative analysis of cytostatic and antibacterial activity of extracts of ambrosia artemisiifolia and other medicinal plants and determination of lithic activity of extracts of ambrosia artemisiifolia

2019 ◽  
Vol 77 (1) ◽  
pp. 71-75
Author(s):  
A. Megalinska ◽  
M. Sokulska
Keyword(s):  
Antibacterial Activity ◽  
Comparative Analysis ◽  
Medicinal Plants ◽  
Water Extract ◽  
Viscum Album ◽  
Ambrosia Artemisiifolia ◽  
Raw Material ◽  
Cytostatic Activity ◽  
Chelidonium Majus

The article is devoted to the important problem of the search of herbal preparations with high antibacterial, cytostatic and lithic activity. The plants such as Ambrosia artemisiifolia, which belongs to the family of Aystrou, are promising in this direction. The aim of the presented work is to study the antibacterial, cytostatic and lithic activity of water extracts of Ambrosia polynolithic and conduct a comparative analysis of these properties with other medicinal plants. Determining that the lectin extract from the green shoots (until flowering) of Ambrosia artemisiifolia does not agglutinate red blood cells, makes it possible to consider this raw material as a potential drug in pharmacology. Investigation of cytostatic activity by the method of Ivanov and Bystrova enabled to establish a place of ambrosia among already well-known medicinal plants. The cytostatic activity of the investigated plants can be represented by the following series in the order of reduction: Chelidónium május, Ambrosia artemisiifolia, Vinca minor, Viscum album. The establishment of antibacterial activity allowed to confirm the presence of anti-staphylococcal activity of the water extract of ambrosia and its significant effect on the colon. Anti-staphylococcus activity of the water extract of Ambrosia artemisiifolia is close to Viscum album and Chelidónium május. The antibacterial effect against Proteus vulgaris was the highest in the extract of аmbrosiа shoots, compared with other medicinal plants studied. Taking into account the phylogenetic method, it could be expected that the plants of the Aystroe family may have a lithic activity with respect to the concrements that are formed in the kidneys of man. So, a study was conducted on the lithic activity of polystyrene embryos and it was found that Ambrosia artemisiifolia has a high lithic activity in relation to urate and oxalate concretions.

HPLC Method for the Determination of the Purity of K[Pt(NH3)Cl3], a Precursor of the Platinum Complexes with Cytostatic Activity

2011 ◽  
Vol 44 (12) ◽  
pp. 2182-2193 ◽  
Author(s):  
Lucie Potucká ◽  
Jitka Housková ◽  
Kamila Syslová ◽  
Roman Hamtil ◽  
Petr Ka[cbreve]er ◽  
...  
Keyword(s):  
Platinum Complexes ◽  
Hplc Method ◽  
Cytostatic Activity

A method using 3-O-methyl-D-glucose and phloretin for the determination of intracellular water space of cells in monolayer culture

1975 ◽  
Vol 68 (2) ◽  
pp. 537-544 ◽  
Author(s):  
Rolf F. Kletzien ◽  
Michael W. Pariza ◽  
Joyce E. Becker ◽  
Van R. Potter
Keyword(s):  
Monolayer Culture ◽  
Intracellular Water ◽  
Water Space

Galactic orbits of stars

1966 ◽  
Vol 25 ◽  
pp. 93-97
Author(s):  
Richard Woolley
Keyword(s):  
Globular Cluster ◽  
Potential Field ◽  
High Velocity ◽  
Velocity Vector ◽  
Variable Stars ◽  
The Sun ◽  
Proper Motions ◽  
Rr Lyrae ◽  
The Galaxy

It is now possible to determine proper motions of high-velocity objects in such a way as to obtain with some accuracy the velocity vector relevant to the Sun. If a potential field of the Galaxy is assumed, one can compute an actual orbit. A determination of the velocity of the globular clusterωCentauri has recently been completed at Greenwich, and it is found that the orbit is strongly retrograde in the Galaxy. Similar calculations may be made, though with less certainty, in the case of RR Lyrae variable stars.

Distance to SN 1987A and the LMC

1999 ◽  
Vol 190 ◽  
pp. 549-554
Author(s):  
Nino Panagia
Keyword(s):  
Angular Size ◽  
Large Magellanic Cloud ◽  
Magellanic Cloud ◽  
Light Curves ◽  
Distance Modulus ◽  
Absolute Size ◽  
Sn 1987A

Using the new reductions of the IUE light curves by Sonneborn et al. (1997) and an extensive set of HST images of SN 1987A we have repeated and improved Panagia et al. (1991) analysis to obtain a better determination of the distance to the supernova. In this way we have derived an absolute size of the ringRabs= (6.23 ± 0.08) x 1017cm and an angular sizeR″ = 808 ± 17 mas, which give a distance to the supernovad(SN1987A) = 51.4 ± 1.2 kpc and a distance modulusm–M(SN1987A) = 18.55 ± 0.05. Allowing for a displacement of SN 1987A position relative to the LMC center, the distance to the barycenter of the Large Magellanic Cloud is also estimated to bed(LMC) = 52.0±1.3 kpc, which corresponds to a distance modulus ofm–M(LMC) = 18.58±0.05.

International determination of the total motion of the pole

1961 ◽  
Vol 13 ◽  
pp. 29-41
Author(s):  
Wm. Markowitz
Keyword(s):  
Secular Motion ◽  
The Future

A symposium on the future of the International Latitude Service (I. L. S.) is to be held in Helsinki in July 1960. My report for the symposium consists of two parts. Part I, denoded (Mk I) was published [1] earlier in 1960 under the title “Latitude and Longitude, and the Secular Motion of the Pole”. Part II is the present paper, denoded (Mk II).

Time and Latitude in South Africa

1972 ◽  
Vol 1 ◽  
pp. 27-38
Author(s):  
J. Hers
Keyword(s):  
South Africa ◽  
Cape Town ◽  
Astronomical Observations ◽  
Royal Observatory ◽  
The Republic ◽  
Astronomical Determination ◽  
Limited Accuracy ◽  
Better Than

In South Africa the modern outlook towards time may be said to have started in 1948. Both the two major observatories, The Royal Observatory in Cape Town and the Union Observatory (now known as the Republic Observatory) in Johannesburg had, of course, been involved in the astronomical determination of time almost from their inception, and the Johannesburg Observatory has been responsible for the official time of South Africa since 1908. However the pendulum clocks then in use could not be relied on to provide an accuracy better than about 1/10 second, which was of the same order as that of the astronomical observations. It is doubtful if much use was made of even this limited accuracy outside the two observatories, and although there may – occasionally have been a demand for more accurate time, it was certainly not voiced.

Large-scale Motion of Solar Filaments

2000 ◽  
Vol 179 ◽  
pp. 205-208
Author(s):  
Pavel Ambrož ◽  
Alfred Schroll
Keyword(s):  
Magnetic Flux ◽  
Proper Motion ◽  
Time Scale ◽  
Large Scale ◽  
Accuracy Of Measurements ◽  
Solar Filaments ◽  
General Movement ◽  
Scale Motion ◽  
Velocity Scatter

AbstractPrecise measurements of heliographic position of solar filaments were used for determination of the proper motion of solar filaments on the time-scale of days. The filaments have a tendency to make a shaking or waving of the external structure and to make a general movement of whole filament body, coinciding with the transport of the magnetic flux in the photosphere. The velocity scatter of individual measured points is about one order higher than the accuracy of measurements.

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