Visualization and identification of hepatitis C viral particles by atomic force microscopy combined with MS/MS analysis

2010 ◽  
Vol 4 (1) ◽  
pp. 15-24 ◽  
Author(s):  
A. L. Kaysheva ◽  
Yu. D. Ivanov ◽  
V. G. Zgoda ◽  
P. A. Frantsuzov ◽  
T. O. Pleshakova ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Hepatitis C ◽  
Force Microscopy ◽  
Atomic Force ◽  
Ms Analysis ◽  
Viral Particles

scholarly journals Visualization and identification of hepatitis c viral particles by atomic force microscopy combined with ms/ms analysis

2010 ◽  
Vol 56 (1) ◽  
pp. 26-39 ◽  
Author(s):  
A.L. Kaysheva ◽  
Yu.D. Ivanov ◽  
V.G. Zgoda ◽  
P.A. Frantsuzov ◽  
T.O. Pleshakova ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Hepatitis C ◽  
Protein Complexes ◽  
Mass Spectrometric ◽  
Force Microscopy ◽  
Atomic Force ◽  
Spectrometric Identification ◽  
Detection And Identification ◽  
Viral Particles ◽  
Mass Spectrometric Identification

Possibility of detection and identification of hepatitis C viral particles with mass spectrometry (MS) in combination with atomic force microscopy (AFM) had been investigated. AFM/MS approach is based on two technologies: 1. AFM-biospecific fishing that allows to detect, concentrate from solution and to count protein complexes on a surface of AFM-nanochip; 2. mass spectrometric identification of these complexes. AFM-biospecific fishing of HCVcoreAg from solution was carried onto surface of AFM-nanochips with immobilized anti-HCVcoreAg. It was shown that HCVcoreAg/anti-HCVcoreim complexes were formed onto AFM-nanochips in quantity sufficient for mass spectrometric identification. Thus, AFM/MS approach allows to identify fragments of hepatitis C virus fished onto a surface of AFM-nanochip from serum.

scholarly journals Detection of hepatitis C virus core protein in serum by atomic force microscopy combined with mass spectrometry

2015 ◽  
pp. 1597 ◽  
Author(s):  
Ivan Shumov ◽  
Yuri Ivanov ◽  
Anna Kaysheva ◽  
Pavel Frantsuzov ◽  
Tatyana Pleshakova ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Atomic Force Microscopy ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Core Protein ◽  
Force Microscopy ◽  
Virus Core ◽  
Atomic Force

scholarly journals Probing the Enzymatic Activity of Individual Biocatalytic fd-Viral Particles by Electrochemical-Atomic Force Microscopy

ACS Catalysis ◽  
2020 ◽  
Vol 10 (14) ◽  
pp. 7843-7856
Author(s):  
Telmo O. Paiva ◽  
Kristian Torbensen ◽  
Anisha N. Patel ◽  
Agnès Anne ◽  
Arnaud Chovin ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Enzymatic Activity ◽  
Force Microscopy ◽  
Atomic Force ◽  
Viral Particles

scholarly journals A magnesium-induced RNA conformational switch at the internal ribosome entry site of hepatitis C virus genome visualized by atomic force microscopy

2014 ◽  
Vol 43 (1) ◽  
pp. 565-580 ◽  
Author(s):  
Ana García-Sacristán ◽  
Miguel Moreno ◽  
Ascensión Ariza-Mateos ◽  
Elena López-Camacho ◽  
Rosa M. Jáudenes ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Internal Ribosome Entry Site ◽  
Virus Genome ◽  
Entry Site ◽  
Conformational Switch ◽  
Internal Ribosome Entry ◽  
Force Microscopy ◽  
Atomic Force

Label-Free DNA Detection of Hepatitis C Virus Based on Modified Conducting Polypyrrole Films at Microelectrodes and Atomic Force Microscopy Tip-Integrated Electrodes

2008 ◽  
Vol 80 (1) ◽  
pp. 237-245 ◽  
Author(s):  
Carla dos Santos Riccardi ◽  
Christine Kranz ◽  
Janusz Kowalik ◽  
Hideko Yamanaka ◽  
Boris Mizaikoff ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Dna Detection ◽  
Label Free ◽  
Force Microscopy ◽  
Polypyrrole Films ◽  
Atomic Force ◽  
Free Dna ◽  
Conducting Polypyrrole

Characterization of Hepatitis C Virus Core Protein Dimerization by Atomic Force Microscopy

2018 ◽  
Vol 90 (7) ◽  
pp. 4596-4602 ◽  
Author(s):  
Wenhui Li ◽  
Xiaolong Kou ◽  
Jiachao Xu ◽  
Wei Zhou ◽  
Rong Zhao ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Core Protein ◽  
Protein Dimerization ◽  
Force Microscopy ◽  
Virus Core ◽  
Atomic Force

scholarly journals HIV-1 specifically traps CD9 and CD81 tetraspanins within viral buds and induces their membrane depletion

2018 ◽  
Author(s):  
Selma Dahmane ◽  
Christine Doucet ◽  
Antoine Le Gall ◽  
Célia Chamontin ◽  
Patrice Dosset ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Plasma Membrane ◽  
Super Resolution ◽  
Induced Membrane ◽  
Force Microscopy ◽  
Atomic Force ◽  
Membrane Topography ◽  
Viral Particles ◽  
Super Resolution Microscopy ◽  
Hiv 1

SUMMARYHIV-1 assembly specifically alters both partitioning and dynamics of the tetraspanins CD9 and CD81 forming enriched areas where the virus buds. Importantly the presence of these proteins at exit sites and in viral particles inhibits virus-induced membrane fusion. To get molecular insights into tetraspanins partitioning in this viral context, we correlated nanoscale CD9 mapping obtained by super resolution microscopy to membrane topography probed by Atomic Force Microscopy (AFM). We demonstrated that CD9 is specifically trapped within the nascent viral particles, especially at buds tips, and that Gag mediate CD9 and CD81 depletion from cellular surfaces, even in the absence of Vpu and Nef, resulting from tetraspanins escaping from the plasma membrane during HIV-1 release. In addition, we showed that CD9 is organized as small membrane assemblies of few tens of nanometers that can coalesce upon Gag expression. Our results support a functional redundancy among tetraspanins during HIV release.

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