scholarly journals Visualization and identification of hepatitis c viral particles by atomic force microscopy combined with ms/ms analysis

2010 ◽  
Vol 56 (1) ◽  
pp. 26-39 ◽  
Author(s):  
A.L. Kaysheva ◽  
Yu.D. Ivanov ◽  
V.G. Zgoda ◽  
P.A. Frantsuzov ◽  
T.O. Pleshakova ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Hepatitis C ◽  
Protein Complexes ◽  
Mass Spectrometric ◽  
Force Microscopy ◽  
Atomic Force ◽  
Spectrometric Identification ◽  
Detection And Identification ◽  
Viral Particles ◽  
Mass Spectrometric Identification

Possibility of detection and identification of hepatitis C viral particles with mass spectrometry (MS) in combination with atomic force microscopy (AFM) had been investigated. AFM/MS approach is based on two technologies: 1. AFM-biospecific fishing that allows to detect, concentrate from solution and to count protein complexes on a surface of AFM-nanochip; 2. mass spectrometric identification of these complexes. AFM-biospecific fishing of HCVcoreAg from solution was carried onto surface of AFM-nanochips with immobilized anti-HCVcoreAg. It was shown that HCVcoreAg/anti-HCVcoreim complexes were formed onto AFM-nanochips in quantity sufficient for mass spectrometric identification. Thus, AFM/MS approach allows to identify fragments of hepatitis C virus fished onto a surface of AFM-nanochip from serum.

scholarly journals Mass Spectrometric Identification of Proteins Enhanced by the Atomic Force Microscopy Immobilization Surface

2021 ◽  
Vol 22 (1) ◽  
pp. 431
Author(s):  
Anna L. Kaysheva ◽  
Pavel A. Frantsuzov ◽  
Arthur T. Kopylov ◽  
Tatyana O. Pleshakova ◽  
Alexander A. Stepanov ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Atomic Force Microscopy ◽  
Ion Trap ◽  
Time Of Flight ◽  
Mass Spectrometric ◽  
Triple Quadrupole ◽  
Target Proteins ◽  
Force Microscopy ◽  
Atomic Force ◽  
Mass Spectrometric Identification

An approach to highly-sensitive mass spectrometry detection of proteins after surface-enhanced concentrating has been elaborated. The approach is based on a combination of mass spectrometry and atomic force microscopy to detect target proteins. (1) Background: For this purpose, a technique for preliminary preparation of molecular relief surfaces formed as a result of a chemical or biospecific concentration of proteins from solution was developed and tested on several types of chip surfaces. (2) Methods: mass spectrometric identification of proteins using trailing detectors: ion trap, time of flight, orbital trap, and triple quadrupole. We used the electrospray type of ionization and matrix-assisted laser desorption/ionization. (3) Results: It is shown that when using locally functionalized atomically smooth surfaces, the sensitivity of the mass spectrometric method increases by two orders of magnitude as compared with measurements in solution. Conclusions: It has been demonstrated that the effective concentration of target proteins on specially prepared surfaces increases the concentration sensitivity of mass spectrometric detectors—time-of-flight, ion trap, triple quadrupole, and orbital ion trap in the concentration range from up to 10−15 M.

Visualization and identification of hepatitis C viral particles by atomic force microscopy combined with MS/MS analysis

2010 ◽  
Vol 4 (1) ◽  
pp. 15-24 ◽  
Author(s):  
A. L. Kaysheva ◽  
Yu. D. Ivanov ◽  
V. G. Zgoda ◽  
P. A. Frantsuzov ◽  
T. O. Pleshakova ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Hepatitis C ◽  
Force Microscopy ◽  
Atomic Force ◽  
Ms Analysis ◽  
Viral Particles

scholarly journals Detection of hepatitis C virus core protein in serum by atomic force microscopy combined with mass spectrometry

2015 ◽  
pp. 1597 ◽  
Author(s):  
Ivan Shumov ◽  
Yuri Ivanov ◽  
Anna Kaysheva ◽  
Pavel Frantsuzov ◽  
Tatyana Pleshakova ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Atomic Force Microscopy ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Core Protein ◽  
Force Microscopy ◽  
Virus Core ◽  
Atomic Force

Atomic Force Microscopy on RNA-Protein Complexes related to RNAi

2005 ◽  
Vol 2005 (Fall) ◽  
Author(s):  
Nils Anspach ◽  
Blagovesta Popova ◽  
Christian Hammann ◽  
Lukasz Jaskiewicz ◽  
Witold Filipowicz ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Protein Complexes ◽  
Force Microscopy ◽  
Atomic Force ◽  
Rna Protein Complexes

scholarly journals Probing the Enzymatic Activity of Individual Biocatalytic fd-Viral Particles by Electrochemical-Atomic Force Microscopy

ACS Catalysis ◽  
2020 ◽  
Vol 10 (14) ◽  
pp. 7843-7856
Author(s):  
Telmo O. Paiva ◽  
Kristian Torbensen ◽  
Anisha N. Patel ◽  
Agnès Anne ◽  
Arnaud Chovin ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Enzymatic Activity ◽  
Force Microscopy ◽  
Atomic Force ◽  
Viral Particles

scholarly journals Atomic force microscopy of DNA-colloidal gold and DNA-protein complexes

1993 ◽  
Author(s):  
Luming Niu ◽  
Wenling Shaiu ◽  
James Vesenka ◽  
Drena D. Larson ◽  
Eric Henderson
Keyword(s):  
Atomic Force Microscopy ◽  
Colloidal Gold ◽  
Protein Complexes ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals A magnesium-induced RNA conformational switch at the internal ribosome entry site of hepatitis C virus genome visualized by atomic force microscopy

2014 ◽  
Vol 43 (1) ◽  
pp. 565-580 ◽  
Author(s):  
Ana García-Sacristán ◽  
Miguel Moreno ◽  
Ascensión Ariza-Mateos ◽  
Elena López-Camacho ◽  
Rosa M. Jáudenes ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Internal Ribosome Entry Site ◽  
Virus Genome ◽  
Entry Site ◽  
Conformational Switch ◽  
Internal Ribosome Entry ◽  
Force Microscopy ◽  
Atomic Force
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