Selectivity coefficients of some simple and complex inorganic ions for Spheron DEAE

1984 ◽  
Vol 49 (10) ◽  
pp. 2349-2354 ◽  
Author(s):  
František Vláčil ◽  
Karel Koňák
Keyword(s):  
Ion Exchange ◽  
Mobile Phase ◽  
Dynamic Method ◽  
Precious Metals ◽  
Inorganic Ions ◽  
Chromatographic Determination ◽  
Chloride Ions ◽  
Chloro Complexes ◽  
Selectivity Coefficients

The selectivity coefficients of the nitrate and chloride ions and of anionic chloro complexes of Au(III), Rh(III), Pd(II), and Pt(IV) for ion exchange on Spheron DEAE in the chloride form are determined by the dynamic method. the complex anion species formed are identified and the ion exchange nature of the sorption of precious metals on this sorbent is confirmed based on the elution order of the precious metals as determined previously by the column chromatography on Spheron DEAE using hydrochloric acid as the mobile phase. The effect of the presence of perchlorate in the mobile phase during the liquid chromatography of precious metals and during the chromatographic determination of nitrate traces is explained.

scholarly journals Robustness evaluation of the chromatographic determination of verapamil hydrochloride

2017 ◽  
Author(s):  
L. S. Logoyda
Keyword(s):  
Analytical Methods ◽  
Mobile Phase ◽  
Chromatographic Method ◽  
Final Analysis ◽  
Chromatographic Determination ◽  
Validation Process ◽  
Verapamil Hydrochloride ◽  
A Value ◽  
Analytical Parameter

The aim of this study was the rubustness evaluation of the chromatographic determination of verapamil hydrochloride using Youden’s test.Methods: Youden’s test is a reliable method to evaluate the robustness of analytical methods, by means of an experiment design which involves seven analytical parameters combined in eight tests. In the present study, we assessed the robustness of a chromatographic method to quantify verapamil hydrochloride using Youden’s test. Hence, it was possible to determine the effect of each analytical parameter in the final analysis results. Youden’s test showed to be a simple and feasible procedure to evaluate the robustness of chromatographic methods.Results: Using the criteria of Youden’s test, the chromato­graphic method showed to be highly robust regarding the verapamil hydrochloride content, when variations in seven analytical parameters were introduced. The highest variation in the verapamil hydrochloride content was 0.26 %, when the concen­tration of triethylamine in the mobile phase was altered; a value considerably low and not significant in routine analyses.Conclusion: Youden’s test showed to be a reliable and useful tool for the robustness evaluation of the chromatographic method for verapamil hydrochloride quantitation. By means of this test, it was possible to evaluate the effect of seven analytical parameters in the final result of the analyses. Therefore, Youden’s test can be successfully applied for the ro­bustness evaluation in validation process of analytical methods by HPLC.

Improved liquid-chromatographic determination of haloperidol in plasma.

1984 ◽  
Vol 30 (7) ◽  
pp. 1228-1230 ◽  
Author(s):  
A K Dhar ◽  
H Kutt
Keyword(s):  
Mobile Phase ◽  
High Performance ◽  
Room Temperature ◽  
Internal Standard ◽  
Isoamyl Alcohol ◽  
Chromatographic Determination ◽  
Reversed Phase ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract This method for determination of haloperidol in plasma is based on "high-performance" isocratic liquid chromatography with the use of a C8 bonded reversed-phase column at room temperature. Haloperidol and the internal standard (chloro-substituted analog) are extracted from alkalinized plasma into isoamyl alcohol/heptane (1.5/98.5 by vol) and back-extracted into dilute H2SO4. The aqueous phase is directly injected onto the column. The mobile phase is a 30/45/25 (by vol) mixture of phosphate buffer (16.5 mmol/L, pH 7.0), acetonitrile, and methanol. Unlike other liquid-chromatographic procedures for haloperidol, commonly used psychotropic drugs do not interfere. Analysis can be completed within an hour. The procedure is extremely sensitive (1.0 microgram/L) and is well reproducible (CV 5.6% for a 2.5 micrograms/L concentration in plasma).

Liquid Chromatographic Determination of Carbofuran in Technical and Formulated Products: Collaborative Study

1986 ◽  
Vol 69 (5) ◽  
pp. 915-918
Author(s):  
Edward J Kikta ◽  
◽  
E Bane ◽  
A Burns ◽  
A Christensen ◽  
...  
Keyword(s):  
Mobile Phase ◽  
Collaborative Study ◽  
Internal Standard ◽  
Chromatographic Determination ◽  
Data Sets ◽  
Matched Pairs ◽  
Reverse Phase ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A liquid chromatographic (LC) method for the analysis of technical and formulated carbofuran samples was evaluated in a collaborative study. Carbofuran is determined by reverse phase LC, using a water-methanol mobile phase and acetophenone as internal standard, and detected at 280 nm. Twelve samples, 5 formulations and technical matched pairs, were analyzed by 17 collaborating laboratories. Accuracy and variability of results are typical of large LC data sets. The method has been adopted official first action.

Liquid Chromatographic Determination of Ergot Alkaloids in Wheat

1986 ◽  
Vol 69 (4) ◽  
pp. 697-699
Author(s):  
George M Ware ◽  
Allen S Carman ◽  
Octave J Francis ◽  
Shia S Kuan
Keyword(s):  
Mobile Phase ◽  
Ergot Alkaloids ◽  
Ammonium Phosphate ◽  
Ammonium Hydroxide ◽  
Chromatographic Determination ◽  
Resin Column ◽  
Fluorescence Detector ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A method is described for the determination of individual ergot alkaloids in wheat. The sample is extracted with ethyl acetate-4% ammonium hydroxide (100 + 10), and the extract is cleaned up by liquidliquid partition. The ergot alkaloids are resolved by liquid chromatography (LC), using a porous cross-linked polystyrene-divinylbenzene resin column and a mobile phase consisting of acetonitrile-0.05M dibasic ammonium phosphate (55 + 45) buffered at pH 10.0. The ergot alkaloids ergonovine, ergonovinine, ergotamine, ergotaminine, α-ergocryptine, α-ergocryptinine, ergocristine, and ergocristinine are separated by LC and detected with a fluorescence detector. Recovery of ergot alkaloids added to wheat at levels of 16-760 ng/g averaged 85.6% with a coefficient of variation of 11.1%.

scholarly journals Micellar Liquid Chromatographic Determination of Carbaryl and 1-Naphthol in Water, Soil, and Vegetables

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Mei-Liang Chin-Chen ◽  
Maria Rambla-Alegre ◽  
Abhilasha Durgbanshi ◽  
Devasish Bose ◽  
Sandeep K. Mourya ◽  
...  
Keyword(s):  
Mobile Phase ◽  
Limit Of Detection ◽  
Cetylpyridinium Chloride ◽  
Sodium Dodecyl ◽  
Chromatographic Determination ◽  
Limit Of Quantification ◽  
Chromatographic Procedure ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

A liquid chromatographic procedure has been developed for the determination of carbaryl, a phenyl-N-methylcarbamate, and its main metabolite 1-naphthol, using a C18 column (250’mm’ × ’4.6’mm) with a micellar mobile phase and fluorescence detection at maximum excitation/emission wavelengths of 225/333’nm, respectively. In the optimization step, surfactants sodium dodecyl sulphate (SDS), Brij-35 andN-cetylpyridinium chloride monohydrate, and organic solvents propanol, butanol, and pentanol were considered. The selected mobile phase was 0.15’M SDS-6% (v/v)-pentanol-0.01’M NaH2PO4buffered at pH 3. Validation studies, according to the ICH Tripartite Guideline, included linearity (r>0.999), limit of detection (5 and 18’ng mL-1, for carbaryl and 1-naphthol, resp.), and limit of quantification (15 and 50’ng mL-1, for carbaryl and 1-naphthol, resp.), with intra- and interday precisions below 1%, and robustness parameters below 3%. The results show that the procedure was adequate for the routine analysis of these two compounds in water, soil, and vegetables samples.

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