Peptidomimetic Inhibitors of Extracellular Aspartic Proteinases of Candida albicans and Candida tropicalis

1999 ◽  
Vol 64 (1) ◽  
pp. 130-137 ◽  
Author(s):  
Kateřina Brožková ◽  
Ivana Křížová ◽  
Libuše Pavlíčková ◽  
Martin Hradilek ◽  
Martin Fusek ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Binding Energy ◽  
Candida Tropicalis ◽  
Enzyme Inhibitor ◽  
Side Chains ◽  
Peptide Substrate ◽  
Aspartic Proteinases ◽  
Scissile Bond ◽  
Protected Peptides

In an attempt to develop effective inhibitors of Candida secreted aspartic proteinases, we have prepared a series of N-protected peptides varying in the type of scissile bond replacement, in the P and P' side chains as well as in the N- and C-terminal modifications. The compounds were tested in vitro with the chromogenic peptide substrate using purified secreted proteinases of C. albicans and C. tropicalis. Our results have confirmed that the binding of inhibitors and their effectiveness is influenced by a number of enzyme-inhibitor interactions. Moreover, factors like solvation/desolvation contribute to the optimal binding energy of the inhibitors.

scholarly journals Synergistic Activity of Chloroquine with Fluconazole against Fluconazole-Resistant Isolates of Candida Species

2014 ◽  
Vol 59 (2) ◽  
pp. 1365-1369 ◽  
Author(s):  
Yali Li ◽  
Zhe Wan ◽  
Wei Liu ◽  
Ruoyu Li
Keyword(s):  
Candida Albicans ◽  
Synergistic Effect ◽  
Candida Tropicalis ◽  
Candida Krusei ◽  
Synergistic Activity ◽  
Broth Microdilution ◽  
Content Type ◽  
Fluconazole Resistant ◽  
Susceptibility Tests

ABSTRACTThein vitroactivity of chloroquine and the interactions of chloroquine combined with fluconazole against 37Candidaisolates were tested using the broth microdilution, disk diffusion, and Etest susceptibility tests. Synergistic effect was detected with 6 of 9 fluconazole-resistantCandida albicansisolates, withCandida kruseiATCC 6258, and with all 12 fluconazole-resistantCandida tropicalisisolates.

Extracellular Aspartic Proteinases from Candida albicans, Candida tropicalis, and Candida parapsilosis Yeasts Differ Substantially in Their Specificities

Biochemistry ◽  
1994 ◽  
Vol 33 (32) ◽  
pp. 9791-9799 ◽  
Author(s):  
Martin Fusek ◽  
Elizabeth A. Smith ◽  
Michael Monod ◽  
Ben M. Dunn ◽  
Stephen I. Foundling
Keyword(s):  
Candida Albicans ◽  
Candida Tropicalis ◽  
Candida Parapsilosis ◽  
Aspartic Proteinases

scholarly journals Aspectos micológicos e suscetibilidade in vitro de leveduras do gênero Candida em pacientes HIV-positivos provenientes do Estado de Mato Grosso

2010 ◽  
Vol 43 (6) ◽  
pp. 673-677 ◽  
Author(s):  
Olivia Cometti Favalessa ◽  
Marilena dos Anjos Martins ◽  
Rosane Christine Hahn
Keyword(s):  
Candida Albicans ◽  
Candida Tropicalis ◽  
Candida Glabrata ◽  
Candida Parapsilosis ◽  
Candida Guilliermondii ◽  
Candida Krusei ◽  
Mato Grosso ◽  
Candida Sp

INTRODUÇÃO: A candidíase é uma das infecções fúngicas mais frequentes entre os pacientes infectados pelo vírus da imunodeficiência humana. O presente estudo objetivou a caracterização das leveduras do gênero Candida de distintas amostras clínicas, provenientes de pacientes HIV - positivos, assim como a determinação do perfil de suscetibilidade in vitro a cinco drogas antifúngicas. MÉTODOS: A caracterização dos isolados de Candida sp foi realizada através da metodologia clássica, testes bioquímicos (zimograma e auxanograma) e morfológicos (prova do tubo germinativo e microcultivo em lâmina). Também, foram realizadas a técnica genotípica (PCR) e identificação pelo método comercial API 20C AUX (BioMeriéux). Para a determinação do perfil de suscetibilidade in vitro, foram utilizadas cinco drogas antifúngicas (cetoconazol, fluconazol, itraconazol, voriconazol e anfotericina B), através do método comercialmente disponível - Etest. RESULTADOS: Foram identificados 105 isolados de leveduras do gênero Candida provenientes de 102 pacientes infectados pelo vírus HIV. Destes, foram caracterizadas 82 (78,1%) Candida albicans, 8 (7,6%) Candida parapsilosis, 8 (7,6%) Candida tropicalis, 4 (3,8%) Candida krusei, 2 (1,9%) Candida glabrata e 1 (1%) Candida guilliermondii. CONCLUSÕES: Considerando o perfil geral de sensibilidade, 60% dos isolados foram suscetíveis a todos os antifúngicos testados, porém as espécies C. tropicalis e C. krusei demonstraram uma tendência a valores mais elevados de CIMs para os azóis do que os encontrados paraC. albicans, sugerindo resistência.

scholarly journals INVESTIGAÇÃO DA ATIVIDADE ANTIFÚNGICA DE Mangifera indica L. FRENTE A DIFERENTES ESPÉCIES DE Candida ssp. ASSOCIADAS À CANDIDÍASE VULVOVAGINAL

2015 ◽  
Vol 19 (3) ◽  
Author(s):  
Karla Maria Santos De Oliveira ◽  
Renata Soares Da Silva ◽  
Giani Maria Cavalcante
Keyword(s):  
Candida Albicans ◽  
Candida Tropicalis ◽  
Candida Glabrata ◽  
Mangifera Indica

Candidíase Vaginal é a infecção fúngica oportunista mais comum e importante nas mulheres. O aumento na utilização de drogas antifúngicas, nos últimos anos, tem causado resistência aos medicamentos disponíveis para o tratamento. O objetivo deste estudo foi investigar a atividade antifúngica de Mangifera indica em estirpes associadas à candidíase vaginal. Os testes in vitro foram realizados com o extrato bruto e frações orgânicas contra as estirpes de Candida albicans (URM 4385), Candida glabrata (URM 4264) e Candida tropicalis (URM 4262), gentilmente cedidas pela Micoteca da Universidade Federal de Pernambuco (UFPE), por meio do ensaio de difusão em ágar por meio de poço e determinação da concentração inibitória mínima (CIM). O extrato etanólico foi o mais ativo, com diâmetro de inibição variando entre 25,5 e 18,5 mm, valores semelhantes à droga padrão, não apresentando diferença estatística. A CIM variou de 0,04 e 0,16 mg/ml em microrganismo testado. As frações acetato de etila e metanólica apresentaram atividade antifúngica relevante contra C. glabrata e C. albicans, respectivamente. Estudo químico para a extração e o isolamento dos compostos ativos é recomendado para ensaios in vitro destes compostos para investigar a sua atividade antifúngica.

scholarly journals In Vitro Activity of a New Polyene, SPA-S-843, against Yeasts

1998 ◽  
Vol 42 (11) ◽  
pp. 3012-3013 ◽  
Author(s):  
C. Rimaroli ◽  
T. Bruzzese
Keyword(s):  
Candida Albicans ◽  
Amphotericin B ◽  
Candida Tropicalis ◽  
Candida Glabrata ◽  
Fungicidal Activity ◽  
Candida Krusei ◽  
Water Soluble ◽  
Vitro Activity ◽  
In Vitro Activity

ABSTRACT The in vitro activity of a new water-soluble polyene, SPA-S-843, was evaluated against 116 strains of Candida,Cryptococcus, and Saccharomyces spp. and compared with that of amphotericin B. SPA-S-843 demonstrated better inhibitory activity against all of the yeasts examined and better fungicidal activity against Candida albicans, Candida glabrata, Candida krusei, and Candida tropicalis than did amphotericin B.

Actividad in vitro de la anfotericina B y la anidulafungina sobre biopelículas de Candida albicans y Candida tropicalis

2007 ◽  
Vol 24 (4) ◽  
pp. 272-277 ◽  
Author(s):  
Amparo Valentín ◽  
Emilia Cantón ◽  
Javier Pemán ◽  
Guillermo Quindós
Keyword(s):  
Candida Albicans ◽  
Candida Tropicalis

scholarly journals Penatalaksanaan kandidiasis oral disebabkan Candida tropicalis pada anak dengan gangguan sistemik

2010 ◽  
Vol 9 (2) ◽  
pp. 78
Author(s):  
Cane Lukisari ◽  
Dwi Setyaningtyas ◽  
Mintarsih Djamhari
Keyword(s):  
Candida Albicans ◽  
Incidence Rate ◽  
Candida Tropicalis ◽  
Oral Candidiasis ◽  
Supportive Therapy ◽  
Good Prognosis ◽  
Oral Infection ◽  
Mucosal Infection ◽  
Adherence Ability

Oral candidiasis is an oral mucosal infection caused by Candida albicans (CA) ornon-Candida albicans Candida (NCAC). C. tropicalis (CT) is most virulent NCACbecause it has the most adherence ability to epithelial cells in vitro as well as mediumlevel proteinase secretion. In addition, CT is the second most common colony found inhuman. Although incidence rate of oral infection was low, this species has virulencepotency, lately reported as the mayor cause of candidemia in immunocompromisedpatients. This paper reports and discusses oral candidiasis due to CT in a child whosuffered anemia with clinical sign of malnutrition. Oral nystatin suspension is therapyof choice because of patient well response, and in vitro studies reported lack ofresponse of fluconazole to candidemia due to C.tropicalis. However, nystatin oralsuspension, should be synchronized with hygiene optimalization of elimination ofpredisposition factors, and supportive therapy would provide a good prognosis.

scholarly journals Penatalaksanaan infeksi Candida tropicalis pada penderita median rhomboid glossitis The management of infection of Candida tropicalis at median rhomboid glossitis patient

2011 ◽  
Vol 10 (1) ◽  
pp. 13
Author(s):  
Cane Lukisari ◽  
Kus Harijanti
Keyword(s):  
Candida Albicans ◽  
Candida Tropicalis ◽  
Oral Candidiasis ◽  
Good Prognosis ◽  
Broad Spectrum Antibiotic ◽  
Trigger Factors ◽  
Paper Report ◽  
Medium Level ◽  
Anti Fungal

Oral candidiasis is oral mucosal infectious caused by Candida albicans or non-candida albicans candida (NCAC).Each Candida species infection has same clinical manifestation, the different only in invasive nature and antifungiresponse. C.tropicalis is most virulence NCAC because the most adherence ability to epithelial cells in vitro as wellas medium level proteinase secretion. In addition, C.topicalis is the second most common colony on human. A studyreported of C.tropicalis as causa of 16% hospitalized oral candidiasis, anti fungal drugs and systemic antibioticwas known as trigger factors. This paper report and discuss oral candidiasis due to C.tropicalis in patient, adultmale, with median rhomboid glossitis. The patient also used broad spectrum antibiotic and topical corticosteroidwith imprecise dose. The management of this case was terminated antibiotic and corticosteroid, oral hygieneoptimalization and was given 0.25% chlorhexidine gluconate. However, oral hygiene optimalization, predispositionfactors elimination, and, precise therapy would give a good prognosis.

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