Calculation of thermodynamic data from UV melting curves of triple helical nucleic acids: A comparison of methods

1999 ◽  
Author(s):  
Uwe Parsch ◽  
Joachim W. Engels
Keyword(s):  
Nucleic Acids ◽  
Thermodynamic Data ◽  
Comparison Of Methods ◽  
Melting Curves ◽  
Uv Melting

Automatic Recording of the Melting Curves of Nucleic Acids

1969 ◽  
Vol 65 (3) ◽  
pp. 443-450 ◽  
Author(s):  
SADATO YABUKI ◽  
AKIYOSHI WADA ◽  
KIN-ICHI UEMURA
Keyword(s):  
Nucleic Acids ◽  
Automatic Recording ◽  
Melting Curves

DUPLEX–TRIPLEX TRANSITION IN COMPLEXES OF NUCLEIC ACIDS WITH HOECHST 33258

2021 ◽  
Vol 55 (1 (254)) ◽  
pp. 39-45
Author(s):  
Marine A. Parsadanyan
Keyword(s):  
Ionic Strength ◽  
Nucleic Acids ◽  
Calf Thymus Dna ◽  
Hoechst 33258 ◽  
Groove Binding ◽  
Melting Curves ◽  
Calf Thymus ◽  
High Concentrations

The study of complexes of groove binding ligand Hoechst 33258 (H33258) with Calf Thymus DNA has been carried out. The data obtained revealed that the melting curves of the complexes of H33258 with DNA are monophasic at low ligand concentrations (0 < r ≤ 0.2) and become biphasic at relatively high concentrations (0.2 < r ≤ 0.33). This effect was revealed to depend on the ionic strength of the solution, and can also occur at high concentrations of the ligand. Comparison of the obtained data with the results for poly(rA)-poly(rU) and poly(dA)-poly(dT) shows a coincidence in the case of DNA and poly(rA)-poly(rU), while in the case of poly(dA)-poly(dT) the melting curves become biphasic at low ligand concentrations and actually do not depend on the ionic strength of the solution.

A comparison of methods for blocking staining of nucleic acids

1978 ◽  
Vol 61 (2) ◽  
pp. 192-196 ◽  
Author(s):  
George Clark ◽  
Sandra Meischen
Keyword(s):  
Nucleic Acids ◽  
Comparison Of Methods

scholarly journals Design and In Vitro Evaluation of Splice-Switching Oligonucleotides Bearing Locked Nucleic Acids, Amido-Bridged Nucleic Acids, and Guanidine-Bridged Nucleic Acids

2021 ◽  
Vol 22 (7) ◽  
pp. 3526
Author(s):  
Takenori Shimo ◽  
Yusuke Nakatsuji ◽  
Keisuke Tachibana ◽  
Satoshi Obika
Keyword(s):  
Nucleic Acids ◽  
Exon Skipping ◽  
Secondary Structures ◽  
High Affinity ◽  
Secondary Structure Formation ◽  
Locked Nucleic Acids ◽  
Mrna And Protein Expression ◽  
Melting Analysis ◽  
Uv Melting

Our group previously developed a series of bridged nucleic acids (BNAs), including locked nucleic acids (LNAs), amido-bridged nucleic acids (AmNAs), and guanidine-bridged nucleic acids (GuNAs), to impart specific characteristics to oligonucleotides such as high-affinity binding and enhanced enzymatic resistance. In this study, we designed a series of LNA-, AmNA-, and GuNA-modified splice-switching oligonucleotides (SSOs) with different lengths and content modifications. We measured the melting temperature (Tm) of each designed SSO to investigate its binding affinity for RNA strands. We also investigated whether the single-stranded SSOs formed secondary structures using UV melting analysis without complementary RNA. As a result, the AmNA-modified SSOs showed almost the same Tm values as the LNA-modified SSOs, with decreased secondary structure formation in the former. In contrast, the GuNA-modified SSOs showed slightly lower Tm values than the LNA-modified SSOs, with no inhibition of secondary structures. We also evaluated the exon skipping activities of the BNAs in vitro at both the mRNA and protein expression levels. We found that both AmNA-modified SSOs and GuNA-modified SSOs showed higher exon skipping activities than LNA-modified SSOs but each class must be appropriately designed in terms of length and modification content.

Comparison of methods in the recovery of nucleic acids from archival formalin-fixed paraffin-embedded autopsy tissues

2010 ◽  
Vol 400 (1) ◽  
pp. 110-117 ◽  
Author(s):  
John B.A. Okello ◽  
Jaymi Zurek ◽  
Alison M. Devault ◽  
Melanie Kuch ◽  
Andrew L. Okwi ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Comparison Of Methods ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Autopsy Tissues ◽  
Formalin Fixed
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