scholarly journals AB0663 COMPARING METHODS FOR DETERMINING ANTIBODIES TO SARS-CoV-2 USING A RAPID TEST AND ENZYME-LINKED IMMUNOSORBENT ASSAY

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 1363.3-1364
Author(s):  
G. Gridneva ◽  
E. Aronova ◽  
S. Glukhova ◽  
M. Cherkasova ◽  
B. Belov ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
False Negative ◽  
Connective Tissue Diseases ◽  
Rapid Test ◽  
Test Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Igm Antibodies ◽  
History Of

Background:An accessible and sensitive and sensitive method for determining antibodies to a new coronavirus infection is often the key to timely provision of the necessary medical care to patients with rheumatic diseasesObjectives:Compare methods for determining antibodies to SARS-CoV-2 using a rapid test and enzyme-linked immunosorbent assay (ELISA)Methods:Methods for determining antibodies to SARS-CoV-2 using an express test (Chromatographic express test SARS-CoV-2 IgG / IgM (Xiamen Biotime Biotechnology, China)) and by ELISA (Reagent kit for enzyme immunoassay of class G immunoglobulins and class M to SARS-CoV-2 (Vector-Best, Russia)) were compared.80 patients were included with a diagnosis of rheumatoid arthritis 26 (33%), psoriatic arthritis - 9 (11%), osteoarthritis - 15 (19%), rheumatic heart disease 1 (1%), SLE 2 (3%), deramtomyositis 3 (4%), systemic sclerosis 5 (6%), systemic connective tissue diseases 4 (5%), including Sjogren’s syndrome, spondyloarthritis 15 (19%).17 (21%) denied a history of COVID-19 symptoms. 63 (79%) noted any signs of COVID-19 3.095 ± 1.45 months before the test (Median 3 [2; 4] months). 63 (79%) noted any signs of COVID-19 109 ± 43 days before the test (Median 111 [78; 135] months). The ELISA method was considered the standard.Results:When comparing the results of the express test and the determination of IgG antibodies to SARS-CoV-2 in serum, the following was obtained: the sensitivity of the express test is 99%. When comparing the results of the express test and the determination of IgM antibodies to SARS-CoV-2 in serum, it was obtained: among 66 samples with a negative result by the express test method, IgM was detected in 6 cases by ELISA/ So, 7.5% of 80 samples were false negative. In 3 of 14 samples with a positive result by the express test, IgM by ELISA was not detected. So, 3.75% of 80 samples were false-positive. (Table 1). When comparing the results of the IgM express test and ELISA, the following was obtained: the sensitivity of the express test was 33%, the specificity was 85%.Table 1.Antibodies, express-testAntibodies (ELISA), absentAntibodies (ELISA), presentRowTotalsIgGabsent011Row %0.00%100.00%present37679Row %3.80%96.20%Totals37780IgМabsent60666Row %90.91%9.09%present11314Row %78.57%21.43%Totals71980Conclusion:When comparing the results of the express test and the determination of IgG antibodies to SARS-CoV-2 in serum, the sensitivity of the express test is 99%. Determination of IgM antibodies to SARS-CoV-2 using a rapid test is less reliable than determination using ELISA.Disclosure of Interests:None declared.

scholarly journals Sensitivity and Kinetics of an NS1-Based Zika Virus Enzyme-Linked Immunosorbent Assay in Zika Virus-Infected Travelers from Israel, the Czech Republic, Italy, Belgium, Germany, and Chile

2017 ◽  
Vol 55 (6) ◽  
pp. 1894-1901 ◽  
Author(s):  
Yaniv Lustig ◽  
Hana Zelena ◽  
Giulietta Venturi ◽  
Marjan Van Esbroeck ◽  
Camilla Rothe ◽  
...  
Keyword(s):  
Czech Republic ◽  
Immunosorbent Assay ◽  
Zika Virus ◽  
False Negative ◽  
Cross Reactivity ◽  
Diagnostic Methods ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Kinetics Of

ABSTRACT Serological diagnosis of Zika virus is challenging due to high cross-reactivity of Zika virus with other flavivirus antibodies. Recently, a Zika NS1-based enzyme-linked immunosorbent assay (ELISA) was developed and shown to be highly specific for Zika antibody detection; however, sensitivity was evaluated for only a small number of confirmed Zika-infected patients. In this study, we measured the sensitivity and kinetics of Zika IgM and IgG antibodies using the Zika NS1-based ELISA in 105 samples from 63 returning travelers infected with Zika virus (proven by PCR or neutralization assay) from Israel, Czech Republic, Italy, Belgium, Germany, and Chile. Zika virus IgM was detected from 2 to 42 days post-symptom onset (PSO) with an overall sensitivity of 79% in the first month and 68% until 2 months PSO, while IgG antibodies were detected from 5 days to 3 years PSO with 79% sensitivity. Interestingly, significant differences in IgM sensitivity and IgM detection period were observed between Israeli and European/Chilean Zika-infected travelers, adding to the complexity of Zika infection diagnosis and suggesting that other diagnostic methods should be complemented to reduce false-negative results.

scholarly journals IMMUNOLOGICAL EFFICIENCY OF THE TETANUS TOXOID USE FOR THE URGENT SPECIFIC TETANUS PREVENTION IN PATIENTS WITH THE WOUND INFECTION

2020 ◽  
pp. 54-57
Author(s):  
E. I. Hirka ◽  
M. S. Popov
Keyword(s):  
Wound Infection ◽  
Immunosorbent Assay ◽  
Tetanus Toxoid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Clostridium Tetani ◽  
Test Systems ◽  
Tetanus Immunity

Summary. Aim. The present study aimed to analyze the effectiveness of tetanus toxoid administration during the urgent specific tetanus prevention. Materials and methods. The determination of tetanus immunity levels in patients with the wound infection before immunization, then 2 and 4 weeks after vaccination. Studies of blood sera of patients were carried out in dynamics by enzyme-linked immunosorbent assay using a set of test systems for determining IgG antibodies to tetanus toxoid «Clostridium tetani toxin IgG», ELISA, Nova Tec Immundiagnostica GmbH, Germany. Results of the study were measured in International Units per milliliter (IU / ml). Results. The tetanus toxoid AP-Biolik using for the urgent specific tetanus prevention is induce protective tetanus immunity levels 2 weeks after vaccination and long-term specific immunity protection 4 weeks after vaccination. Conclusions. The immunological efficiency of AP-Biolik using was established during its applying for urgent specific tetanus prevention in patients with the wound infection.

Comparison of an ELISA-based Screening Test with Liquid Chromatography for the Determination of Aflatoxins in Corn

1991 ◽  
Vol 74 (5) ◽  
pp. 827-829
Author(s):  
Rodney W Beaver ◽  
Mary A James ◽  
Tay Y Lin
Keyword(s):  
Liquid Chromatography ◽  
Immunosorbent Assay ◽  
False Positive ◽  
Screening Test ◽  
False Negative ◽  
Screening Method ◽  
Incorrect Response ◽  
False Positives ◽  
Enzyme Linked Immunosorbent Assay

Abstract An enzyme-linked immunosorbent assay (ELISA) screening test (CITE PROBE) was compared to liquid chromatography (LC) for the determination of aflatoxins in naturally contaminated corn samples. The CITE PROBE, with a positive/negative cutoff of 5 ng/g aflatoxin Bi, was correct (based on LC results) on 47 of 51 samples. Two of the Incorrect responses by the CITE PROBE were false positives on samples containing 4.4 ng/g and 4.1 ng/g aflatoxins by LC. Another incorrect response was a false negative on a sample containing 5.5 ng/g aflatoxins by LC. The fourth Incorrect response was a false positive on a sample containing 1.9 ng/g aflatoxins by LC. On the basis of these results, the CITE PROBE was determined to be a reliable screening method for the detection of % 5 ng/g aflatoxins in corn.

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