Clinical significance of an equivocal interferon γ release assay result

2011 ◽  
Vol 96 (2) ◽  
pp. 284-288 ◽  
Author(s):  
Marcus Ang ◽  
Wong Wanling ◽  
Soon-Phaik Chee
Keyword(s):  
Clinical Significance ◽  
Interferon Γ ◽  
Assay Result ◽  
Release Assay

Use of a T Cell Interferon-γ Release Assay to Evaluate Tuberculosis Risk in Newly Qualified Physicians in Singapore Healthcare Institutions

2009 ◽  
Vol 30 (9) ◽  
pp. 870-875 ◽  
Author(s):  
C. B. E. Chee ◽  
L. K. Y. Lim ◽  
T. M. Barkham ◽  
D. R. Koh ◽  
S. O. Lam ◽  
...  
Keyword(s):  
Healthcare Workers ◽  
Latent Tuberculosis ◽  
Risk Groups ◽  
Interferon Γ ◽  
Public Hospitals ◽  
High Rate ◽  
Assay Result ◽  
Release Assay ◽  
Baseline Test ◽  
Tuberculosis Risk

Background.Surveillance for latent tuberculosis in high-risk groups such as healthcare workers is limited by the nonspecificity of the tuberculin skin test (TST) in BCG-vaccinated individuals. The Mycobacterium tuberculosis antigen-specific interferon-γ release assays (IGRAs) show promise for more accurate latent tuberculosis detection in such groups.Objective.To compare the utility of an IGRA, the T-SPOT.TB assay, with that of the TST in healthcare workers with a high rate of BCG vaccination.Methods.Two hundred seven medical students from 2 consecutive cohorts underwent the T-SPOT.TB test and the TST in their final year of study. Subjects with negative baseline test results underwent repeat testing after working for 1 year as junior physicians in Singapore's public hospitals.Results.The baseline TST result was an induration 10 mm or greater in diameter in 177 of the 205 students who returned to have their TST results evaluated (86.3%), while the baseline T-SPOT.TB assay result was positive in 9 (4.3%) of the students. Repeat T-SPOT.TB testing in 182 baseline-negative subjects showed conversion in 9 (4.9%). A repeat TST in 18 subjects with baseline-negative TST results did not reveal any TST result conversion.Conclusions.The high rate of positive baseline TST results in our BCG-vaccinated healthcare workers renders the TST unsuitable as a surveillance tool in this tuberculosis risk group. Use of an IGRA has enabled the detection and treatment of latent tuberculosis in this group. Our T-SPOT.TB conversion rate highlights the need for greater tuberculosis awareness and improved infection control practices in our healthcare institutions.

Einführung des Interferon-γ-Release Assay bei Tuberkulose-Umgebungsuntersuchungen – ein 2-Jahres Projekt in Niedersachsen

2010 ◽  
Vol 73 (06) ◽  
pp. 363-368 ◽  
Author(s):  
D. Wagner ◽  
M. Scharlach ◽  
J. Sielski ◽  
J. Dreesman ◽  
M. Pulz
Keyword(s):  
Interferon Γ ◽  
Release Assay

scholarly journals Feasibility of the Interferon-γ Release Assay for the Diagnosis of Genitourinary Tuberculosis in an Endemic Area

2013 ◽  
Vol 54 (2) ◽  
pp. 123 ◽  
Author(s):  
Jong Keun Kim ◽  
Woo Jin Bang ◽  
Cheol Young Oh ◽  
Changhee Yoo ◽  
Jin Seon Cho
Keyword(s):  
Endemic Area ◽  
Interferon Γ ◽  
Genitourinary Tuberculosis ◽  
Release Assay

scholarly journals Development of an interferon-γ release assay (IGRA) for detection of Brucella abortus and clinical diagnosis of brucellosis

2017 ◽  
Vol 11 (11) ◽  
pp. 847-853 ◽  
Author(s):  
Yu Feng ◽  
Liangquan Zhu ◽  
Xiaowei Peng ◽  
Hui Jiang ◽  
Ge Zhang ◽  
...  
Keyword(s):  
Clinical Diagnosis ◽  
Great Risk ◽  
Brucella Abortus ◽  
Interferon Γ ◽  
Clinical Samples ◽  
Somatic Antigen ◽  
Release Assay ◽  
Ifn Γ ◽  
Negative Controls ◽  
Somatic Antigens

Introduction: Brucellosis, caused by Brucella abortus (B. abortus), is an important zoonosis posing a great risk to both livestock and humans. Currently, most assays for clinical diagnosis of brucellosis have been developed based on serological principles; however, these assays have a number of limitations and disadvantages. Methodology: To address this concern, the aim of this study was to develop a gamma interferon (IFN-γ) release assay (IGRA) for the diagnosis of brucellosis. Towards this end, the stimulating effect induced by different somatic antigens of B. abortus on the secretion of IFN-γ was evaluated. Results: The best antigen candidate, B. abortus strain 2308, able to induce high levels of IFN-γ expression in peripheral blood (PB) cells from cattle, was used for the development of the IGRA. The optimal concentration for stimulation was determined as 1.0×107 CFU/mL. This study demonstrated that IFN-γ was detectable on day 5 post infection (p.i.) and peaked on day 14 p.i.. Finally, the IGRA developed was used for detection of B. abortus in clinical samples, and a higher level of IFN-γ was detected in Brucella-infected samples compared to vaccination samples and negative controls. Conclusions: The optimal somatic antigen for B. abortus was identified and used to establish a robust IGRA. The IGRA developed is suitable for clinical diagnosis of brucellosis, especially in the early stages of infection.

Prediction of the risk of active tuberculosis in HIV-infection with an interferon-γ release assay

The Lancet ◽  
2013 ◽  
Vol 382 ◽  
pp. S16
Author(s):  
Susan Shin-Jung Lee ◽  
Hsi-Hsun Lin ◽  
Hung-Chin Tsai ◽  
Ih-Jen Su ◽  
Cheng-Len Sy ◽  
...  
Keyword(s):  
Hiv Infection ◽  
Interferon Γ ◽  
Active Tuberculosis ◽  
Release Assay

scholarly journals Abdominal tuberculosis with a Pseudo-Sister Mary Joseph nodule mimicking peritoneal carcinomatosis

2019 ◽  
Vol 12 (6) ◽  
pp. e229624 ◽  
Author(s):  
Amine Awad ◽  
Tom Pampiglione ◽  
Zaker Ullah
Keyword(s):  
Transvaginal Ultrasound ◽  
Positive Culture ◽  
Ct Scanning ◽  
Abdominal Tuberculosis ◽  
Tumour Marker ◽  
Interferon Γ ◽  
Ca 125 ◽  
Fluid Analysis ◽  
Release Assay ◽  
Sister Mary Joseph

A 46-year-old woman presented in severe abdominal pain on a background of 3 months of weight loss and intermittent vomiting. She had visited East Africa 6 months prior but reported no unwell contacts. On examination, she had generalised abdominal tenderness, distension and a painful paraumbilical swelling. CT scanning confirmed small bowel obstruction and revealed widespread peritoneal nodules, lymphadenopathy, ascites and a soft tissue paraumbilical mass. CA-125 tumour marker was elevated. However, transvaginal ultrasound scanning showed normal-appearing ovaries. She underwent a diagnostic laparoscopy for ascitic fluid analysis and biopsy of omental and peritoneal nodules, which revealed a lymphocytic exudate and caseating granulomas, respectively. Interferon-γ release assay and repeated stains for acid-fast bacilli were negative. She was commenced on antituberculous chemotherapy for a presumed diagnosis of abdominal tuberculosis. Positive culture results 2 weeks later confirmed Mycobacterium tuberculosis infection. The patient experienced a complete resolution of symptoms within 6 weeks of treatment.

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