scholarly journals In vitro diagnosis of cow's milk protein sensitive enteropathy by organ culture method.

Gut ◽  
1981 ◽  
Vol 22 (3) ◽  
pp. 199-202 ◽  
Author(s):  
N Iyngkaran ◽  
M Yadav ◽  
S Balabaskaran ◽  
E Sumithran
Keyword(s):  
Organ Culture ◽  
Milk Protein ◽  
Culture Method ◽  
Cow’S Milk ◽  
Cow's Milk ◽  
Cow’S Milk Protein ◽  
In Vitro Diagnosis

scholarly journals Peptide Release after Simulated Infant In Vitro Digestion of Dry Heated Cow’s Milk Protein and Transport of Potentially Immunoreactive Peptides across the Caco-2 Cell Monolayer

Nutrients ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 2483 ◽  
Author(s):  
Hannah E. Zenker ◽  
Harry J. Wichers ◽  
Monic M. M. Tomassen ◽  
Sjef Boeren ◽  
Nicolette W. De Jong ◽  
...  
Keyword(s):  
T Cell ◽  
Milk Protein ◽  
Cell Monolayer ◽  
In Vitro Digestion ◽  
Cow’S Milk ◽  
T Cell Epitopes ◽  
Peptide Profile ◽  
Cow's Milk ◽  
Cow’S Milk Protein

Dry heating of cow’s milk protein, as applied in the production of “baked milk”, facilitates the resolution of cow’s milk allergy symptoms upon digestion. The heating and glycation-induced changes of the protein structure can affect both digestibility and immunoreactivity. The immunological consequences may be due to changes in the peptide profile of the digested dry heated milk protein. Therefore, cow’s milk protein powder was heated at low temperature (60 °C) and high temperature (130 °C) and applied to simulated infant in vitro digestion. Digestion-derived peptides after 10 min and 60 min in the intestinal phase were measured using LC-MS/MS. Moreover, digests after 10 min intestinal digestion were applied to a Caco-2 cell monolayer. T-cell epitopes were analysed using prediction software, while specific immunoglobin E (sIgE) binding epitopes were identified based on the existing literature. The largest number of sIgE binding epitopes was found in unheated samples, while T-cell epitopes were equally represented in all samples. Transport of glycated peptide indicated a preference for glucosyl lysine and lactosyl-lysine-modified peptides, while transport of peptides containing epitope structures was limited. This showed that the release of immunoreactive peptides can be affected by the applied heating conditions; however, availability of peptides containing epitopes might be limited.

scholarly journals Kinetics of in Vitro Response to β-Lactoglobulin in Children Allergic to and Tolerant of Cow’s Milk Protein

2016 ◽  
Vol 26 (4) ◽  
pp. 275-277
Author(s):  
L Chini ◽  
V Pacciani ◽  
S Corrente ◽  
E Monteferrario ◽  
ML Romiti ◽  
...  
Keyword(s):  
Milk Protein ◽  
Cow’S Milk ◽  
Cow's Milk ◽  
In Vitro Response ◽  
Cow’S Milk Protein ◽  
Β Lactoglobulin ◽  
Kinetics Of

scholarly journals 52 In Vitro Evidence of Cow's Milk Protein Sensitisation in Neonatal Necrotising Enterocolitis

2004 ◽  
Vol 56 (3) ◽  
pp. 473-473
Author(s):  
S L Chuang ◽  
P Hayes ◽  
E Ogundipe ◽  
N Modi ◽  
M Haddad ◽  
...  
Keyword(s):  
Milk Protein ◽  
Necrotising Enterocolitis ◽  
Cow’S Milk ◽  
Cow's Milk ◽  
Neonatal Necrotising Enterocolitis ◽  
Cow’S Milk Protein

scholarly journals O5 The prevalence of cow’s milk protein allergy in infants with gastroschisis and intestinal failure type 2 and 3

2021 ◽  
Author(s):  
Kornilia Nikaki ◽  
Tracey Johnson ◽  
Haidee Norton ◽  
Gabis Chana ◽  
Amrita Garcha ◽  
...  
Keyword(s):  
Milk Protein ◽  
Intestinal Failure ◽  
Cow’S Milk ◽  
Cow's Milk ◽  
Failure Type ◽  
Cow’S Milk Protein

scholarly journals Fecal Calprotectin and Eosinophil-Derived Neurotoxin in Children with Non-IgE-Mediated Cow’s Milk Protein Allergy

2021 ◽  
Vol 10 (8) ◽  
pp. 1595
Author(s):  
María Roca ◽  
Ester Donat ◽  
Ana Rodriguez Varela ◽  
Eva Carvajal ◽  
Francisco Cano ◽  
...  
Keyword(s):  
Milk Protein ◽  
Statistical Significance ◽  
Functional Gastrointestinal Disorders ◽  
Fecal Calprotectin ◽  
Cow’S Milk ◽  
Cow's Milk ◽  
Young Infants ◽  
Healthy Infants ◽  
Ige Mediated ◽  
Cow’S Milk Protein

Our aim is to assess the efficacy of fecal calprotectin (fCP) and fecal eosinophil-derived neurotoxin (fEDN) as diagnostic markers of cow’s milk protein allergy (CMPA) and for monitoring the infants’ response to a non-IgE mediated cow’s milk protein (CMP)-free diet. We prospectively recruited infants aged 0 to 9 months. Stool samples were taken from 30 infants with CMPA, 19 with mild functional gastrointestinal disorders, 28 healthy infants, and 28 children who presented mild infections. Despite the fact that levels of fCP and fEDN in CMPA infants were higher than in healthy infants at month 0, differences for both parameters did not reach statistical significance (p-value 0.119 and 0.506). After 1 month of an elimination diet, no statistically significant differences in fCP with basal levels were found (p-values 0.184) in the CMPA group. We found a high variability in the fCP and fEDN levels of young infants, and discrepancies in individual behavior of these markers after a CMP-free diet was started. It seems that neither fCP nor fEDN levels are helpful to discriminate between healthy infants and those with signs or symptoms related to non-IgE-mediated CMPA. Additionally, it is debatable if on an individual basis, fCP or fEDN levels could be used for clinical follow-up and dietary compliance monitoring. However, prospective studies with larger populations are needed to draw robust conclusions.

Sign in / Sign up

Export Citation Format

Share Document