scholarly journals Some problems found in HIV-1 RNA quantification

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S. Yoshida
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2009 ◽  
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PLoS ONE ◽  
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David E. Barker

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pp. 76-81 ◽  
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AIDS ◽  
2001 ◽  
Vol 15 (18) ◽  
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Angeles Muñóz-Fernández ◽  
Eduardo Fernández-Cruz

2016 ◽  
Vol 54 (6) ◽  
pp. 1641-1643 ◽  
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Edouard Tuaillon ◽  
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Arnaud Kwimatouo Lekpa Franchard ◽  
Thérèse Kagoné ◽  
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The impact of HIV-1 DNA coamplification during HIV-1 RNA quantification on dried blood spots (DBS) was explored. False-positive HIV RNA detection (22/62, 35%) was associated with high HIV-1 DNA levels. Specificity of HIV-1 RNA assays on DBS should be evaluated following manufacturer protocols on samples with HIV-1 DNA levels of ≥1,000 copies/106peripheral blood mononuclear cells.


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