Eosinophil granule proteins: what they can tell us about asthma

Thorax ◽  
2021 ◽  
pp. thoraxjnl-2021-218268
Author(s):  
Ji-Hyang Lee ◽  
Tae-Bum Kim
Keyword(s):  
Eosinophil Granule Proteins ◽  
Granule Proteins ◽  
Eosinophil Granule

Increased Eosinophil Granule Proteins in Gut Lavage Fluid From Patients With Inflammatory Bowel Disease

1997 ◽  
Vol 72 (2) ◽  
pp. 117-123 ◽  
Author(s):  
Aaron M. Levy ◽  
Gerald J. Gleich ◽  
William J. Sandborn ◽  
William J. Tremaine ◽  
Betty L. Steiner ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Lavage Fluid ◽  
Eosinophil Granule Proteins ◽  
Inflammatory Bowel ◽  
Granule Proteins ◽  
Eosinophil Granule ◽  
Gut Lavage

scholarly journals Dexamethasone and lidocaine suppress eosinophilopoiesis from umbilical cord blood cells

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Masato Muraki ◽  
Hirohito Kita ◽  
Gerald J. Gleich
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Cultured Cells ◽  
Eosinophil Infiltration ◽  
Human Umbilical Cord ◽  
Dependent Manner ◽  
Eosinophil Granule Proteins ◽  
Granule Proteins ◽  
Eosinophil Granule

Abstract Background Eosinophils play an important role in allergic inflammation. Glucocorticosteroids have been used as an anti-inflammatory medication for inflammatory diseases involving eosinophil infiltration. Some effect of nebulized lidocaine has been reported when treating certain patients with asthma, which is also an inflammatory disease. The goal of this study was to examine the effects of dexamethasone and lidocaine on eosinophil proliferation and differentiation using a model of human umbilical cord blood mononuclear cells (UCMC) cultured with IL-5. Methods UCMC were cultured with IL-5 (5 ng/mL) for 4 weeks. The effects of dexamethasone and lidocaine on the number and morphology of eosinophilic cells were visualized with Wright-Giemsa and cyanide-resistant peroxidase stains. Moreover, the effect on eosinophil-derived neurotoxin (EDN) and eosinophil peroxidase (EPX) contents in cultured cells were evaluated using radioimmunoassay. Results The number of eosinophilic cells and EDN and EPX content in cultured cells increased in a time-dependent manner in the presence of IL-5. Dexamethasone treatment slightly decreased the number of eosinophilic cells in one week, but this effect was lost in 2–4 weeks. Macrophages in cultured UCMC treated with dexamethasone contained more eosinophil granule proteins. Both EDN and EPX content in cultured cells were reduced by dexamethasone. Lidocaine decreased the number of eosinophilic cells and reduced both EDN and EPX contents in cultured cells. Conclusions Dexamethasone suppressed the production of eosinophil granule proteins and may also induce apoptosis of eosinophils, while lidocaine suppresses eosinophilopoiesis.

Sign in / Sign up

Export Citation Format

Share Document