Serological response of cattle, sheep and goats in Kenya vaccinated with killed Brucella melitensis strain H38 adjuvant vaccine

1983 ◽  
Vol 112 (20) ◽  
pp. 476-479 ◽  
Author(s):  
S. Waghela
Keyword(s):  
Brucella Melitensis ◽  
Serological Response ◽  
Sheep And Goats ◽  
Adjuvant Vaccine

scholarly journals Immunohistochemical Detection of Brucella Melitensis Antigens in Cases of Naturally Occurring Abortions in Sheep

2008 ◽  
Vol 20 (6) ◽  
pp. 803-806 ◽  
Author(s):  
Fatma Ilhan ◽  
Zabit Yener
Keyword(s):  
Kupffer Cells ◽  
Brucella Melitensis ◽  
Immunohistochemical Detection ◽  
Zoonotic Pathogen ◽  
Sheep And Goats ◽  
Naturally Occurring ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Microscopic Studies ◽  
Formalin Fixed

Brucella melitensis, a worldwide zoonotic pathogen, is a significant cause of abortion in sheep and goats in some countries. The present study was carried out to determine, by immunohistochemistry, the presence of B. melitensis antigens in 110 naturally occurring aborted sheep fetuses. Sections of lung, liver, kidney, and spleen of each fetus were stained with immunoperoxidase to detect Brucella antigens. Brucella melitensis antigens were detected in 33 of 110 fetuses (30%). In the 33 positive cases, Brucella antigens were found in lung (25 [22.7%]), liver (21 [19%]), spleen (13 [11.8%]), and kidney (6 [5.4%]). Microscopic studies demonstrated that Brucella antigens were mainly located in the cytoplasm of macrophages and neutrophils of the lung, and in the cytoplasm of macrophages in the portal infiltrates and Kupffer cells of the liver. It was concluded that immunohistochemistry in formalin-fixed, paraffin-embedded tissues is a useful tool for the diagnosis of spontaneous ovine abortion caused by B. melitensis.

Efficacy of different Rose Bengal and complement fixation antigens for the diagnosis of Brucella melitensis infection in sheep and goats

1994 ◽  
Vol 134 (16) ◽  
pp. 415-420 ◽  
Author(s):  
J. Blasco ◽  
B. Garin-Bastuji ◽  
C. Marin ◽  
G. Gerbier ◽  
J. Fanlo ◽  
...  
Keyword(s):  
Rose Bengal ◽  
Complement Fixation ◽  
Brucella Melitensis ◽  
Sheep And Goats

Serological response of dromedary camels vaccinated with Brucella abortus RB51 and Brucella melitensis REV 1 Vaccines

2019 ◽  
Vol 26 (2) ◽  
pp. 121
Author(s):  
U Wernery ◽  
M Rodriguez Caveney ◽  
Sh Jose ◽  
B Johnson ◽  
R Raghavan ◽  
...  
Keyword(s):  
Brucella Abortus ◽  
Brucella Melitensis ◽  
Serological Response ◽  
Dromedary Camels

Comparison of two selective media for the isolation of Brucella melitensis from naturally infected sheep and goats

1996 ◽  
Vol 138 (17) ◽  
pp. 409-411 ◽  
Author(s):  
C. M. Marin ◽  
M. P. Jimenez de Bagues ◽  
M. Barberan ◽  
J. M. Blasco
Keyword(s):  
Brucella Melitensis ◽  
Infected Sheep ◽  
Selective Media ◽  
Sheep And Goats

scholarly journals ISOLATION AND MOLECULAR IDENTIFICATION OF BRUCELLA MELITENSIS IN ABORTED FETUSES OF SHEEP AND GOATS IN SULAIMANI PROVINCE, IRAQ

2020 ◽  
Vol 10 (3) ◽  
pp. 257-265
Author(s):  
Othman Jamal Nassrullah ◽  
◽  
Mohammed Omer Mohammad ◽  
Shwan Kamal Rachid ◽  
◽  
...  
Keyword(s):  
Molecular Identification ◽  
Brucella Melitensis ◽  
Sheep And Goats

scholarly journals VirB12 Is a Serological Marker of Brucella Infection in Experimental and Natural Hosts

2007 ◽  
Vol 15 (2) ◽  
pp. 208-214 ◽  
Author(s):  
Hortensia G. Rolán ◽  
Andreas B. den Hartigh ◽  
Melissa Kahl-McDonagh ◽  
Thomas Ficht ◽  
L. Garry Adams ◽  
...  
Keyword(s):  
Brucella Melitensis ◽  
Antibody Responses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serological Response ◽  
Serum Samples ◽  
Positive Serology ◽  
Type Iv ◽  
Natural Hosts ◽  
Species Type

ABSTRACT The Brucella species type IV secretion system, encoded by the virB1-12 locus, is required for intracellular replication and persistent infection in vivo. The requirement of VirB proteins for infection suggests that they are expressed in vivo and may therefore represent serological markers of infection. To test this idea, we purified recombinant VirB1, VirB5, VirB11, and VirB12 and tested for their recognition by antibodies in sera from experimentally infected mice and goats by using an indirect enzyme-linked immunosorbent assay. Antibody responses to VirB12 but not to VirB1, VirB5, or VirB11 were detected in 20/20 mice experimentally inoculated with Brucella abortus and 12/12 goats experimentally infected with Brucella melitensis. The potential use of VirB12 as a serological tool for the diagnosis of brucellosis was evaluated in the natural bovine host. Serum samples from 145 cattle of known serology (29% negative and 71% positive) were analyzed for the production of antibody responses to VirB12. One hundred two cattle samples (70.3%) were positive for antibodies to VirB12, while 43 samples were negative (29.7%). A positive serological response to VirB12 correlated with positive serology to whole B. abortus antigen in 99% of samples tested. These results show that VirB12 is expressed during infection of both experimental and natural hosts of Brucella species, and they suggest that VirB12 may be a useful serodiagnostic marker for brucellosis.

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