Control and Eradication of Brucella melitensis Infection in Sheep and Goats

2011 ◽  
Vol 27 (1) ◽  
pp. 95-104 ◽  
Author(s):  
José M. Blasco ◽  
Baldomero Molina-Flores
Keyword(s):  
Brucella Melitensis ◽  
Sheep And Goats ◽  
Control And Eradication

scholarly journals Immunohistochemical Detection of Brucella Melitensis Antigens in Cases of Naturally Occurring Abortions in Sheep

2008 ◽  
Vol 20 (6) ◽  
pp. 803-806 ◽  
Author(s):  
Fatma Ilhan ◽  
Zabit Yener
Keyword(s):  
Kupffer Cells ◽  
Brucella Melitensis ◽  
Immunohistochemical Detection ◽  
Zoonotic Pathogen ◽  
Sheep And Goats ◽  
Naturally Occurring ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Microscopic Studies ◽  
Formalin Fixed

Brucella melitensis, a worldwide zoonotic pathogen, is a significant cause of abortion in sheep and goats in some countries. The present study was carried out to determine, by immunohistochemistry, the presence of B. melitensis antigens in 110 naturally occurring aborted sheep fetuses. Sections of lung, liver, kidney, and spleen of each fetus were stained with immunoperoxidase to detect Brucella antigens. Brucella melitensis antigens were detected in 33 of 110 fetuses (30%). In the 33 positive cases, Brucella antigens were found in lung (25 [22.7%]), liver (21 [19%]), spleen (13 [11.8%]), and kidney (6 [5.4%]). Microscopic studies demonstrated that Brucella antigens were mainly located in the cytoplasm of macrophages and neutrophils of the lung, and in the cytoplasm of macrophages in the portal infiltrates and Kupffer cells of the liver. It was concluded that immunohistochemistry in formalin-fixed, paraffin-embedded tissues is a useful tool for the diagnosis of spontaneous ovine abortion caused by B. melitensis.

scholarly journals REV-1 conjunctival vaccine administration in Turkey

2021 ◽  
Vol 77 (10) ◽  
pp. 6582-2021
Author(s):  
GULSEN GONCAGUL ◽  
ELCİN GUNAYDİN ◽  
OZLEM KARDOGAN ◽  
YAVUZ COKAL ◽  
EMSAL SİNEM OZDEMİR SALCİ
Keyword(s):  
Small Ruminants ◽  
Subcutaneous Route ◽  
Third Trimester ◽  
Serological Tests ◽  
Vaccine Administration ◽  
Sheep And Goats ◽  
Endemic Areas ◽  
Positive Results ◽  
Vaccination Period ◽  
Control And Eradication

Brucellosis in sheep and goats has a major economic and zoonotic importance, and implementation of strategies for its control and eradication is essential in endemic areas. In this study, the enhanced abortion cases in small ruminants after conjunctival Rev-1 vaccine administration was examined by PCR in terms of probability of vaccine induced abort cases. Of the examined 77 cotyledons from the aborted fetuses belonging to 68 sheep and 9 goats, 70 (90.90%) were found to harbour Brucella spp. DNA. In the first, second and third trimester of gestation, the rate of 100%, 40% and 42.85% positive results were determined from the cotyledons of the small ruminants, respectively. In conclusion, the conjunctival route of Rev-1 vaccine administration was safe during field vaccination, compatible with the serological tests and induces less abortion compared with the subcutaneous route as long as the period of pregnancy is taken into consideration during the vaccination period.

Efficacy of different Rose Bengal and complement fixation antigens for the diagnosis of Brucella melitensis infection in sheep and goats

1994 ◽  
Vol 134 (16) ◽  
pp. 415-420 ◽  
Author(s):  
J. Blasco ◽  
B. Garin-Bastuji ◽  
C. Marin ◽  
G. Gerbier ◽  
J. Fanlo ◽  
...  
Keyword(s):  
Rose Bengal ◽  
Complement Fixation ◽  
Brucella Melitensis ◽  
Sheep And Goats

Comparison of two selective media for the isolation of Brucella melitensis from naturally infected sheep and goats

1996 ◽  
Vol 138 (17) ◽  
pp. 409-411 ◽  
Author(s):  
C. M. Marin ◽  
M. P. Jimenez de Bagues ◽  
M. Barberan ◽  
J. M. Blasco
Keyword(s):  
Brucella Melitensis ◽  
Infected Sheep ◽  
Selective Media ◽  
Sheep And Goats

scholarly journals ISOLATION AND MOLECULAR IDENTIFICATION OF BRUCELLA MELITENSIS IN ABORTED FETUSES OF SHEEP AND GOATS IN SULAIMANI PROVINCE, IRAQ

2020 ◽  
Vol 10 (3) ◽  
pp. 257-265
Author(s):  
Othman Jamal Nassrullah ◽  
◽  
Mohammed Omer Mohammad ◽  
Shwan Kamal Rachid ◽  
◽  
...  
Keyword(s):  
Molecular Identification ◽  
Brucella Melitensis ◽  
Sheep And Goats

scholarly journals INVESTIGATION OF THE HUMAN RESOURCES NEEDS FOR THE EFFECTIVE AND EFFICIENT IMPLEMENTATION OF THE SHEEP AND GOAT BRUCELLOSIS PROGRAM IN GREECE

2019 ◽  
Vol 18 (2) ◽  
Author(s):  
Aristomenis KATSIOLIS ◽  
Olga THANOU ◽  
Myrsini TZANI ◽  
Chrysoula DILE ◽  
Marilina KOROU ◽  
...  
Keyword(s):  
Public Health ◽  
Human Resources ◽  
Control Strategy ◽  
Animal Health ◽  
Efficient Implementation ◽  
External Factors ◽  
Veterinary Services ◽  
Sheep And Goats ◽  
The Poor ◽  
Control And Eradication

The sheep and goats brucellosis program is one of the most important animalhealth programs with significant animal health, public health, and internationaltrade consequences. Several factors are involved in the implementation of thisprogram, while its success depends on the availability of an effective, well-trained,and fully engaged staff. Considering the poor results of sheep and goats brucellosisprogram in Greece mainly related to the limited number of veterinarians workingin the regional veterinary services, the present study aims to approach the numberof the human resources and more precisely of veterinarians that are essential forthe effective and efficient brucellosis control strategy in Greece. Therefore, the dataderived by the performance of the program for the years 2012 to 2015 are analyzed.Finally, economic and political circumstances – external factors that affect thefunctionality of the program as well as all relevant bottlenecks are being discussed.The findings of the present study indicate that approximately 485 veterinarians with485 assistants working for the needs of the brucellosis program would significantlyassist on the complete implementation of the brucellosis control and eradication ofthe disease in Greek sheep and goat holdings.

scholarly journals Molecular Study of the Prevalence of Brucella Abortus and Brucella Melitensis in the Blood and Lymph Node Samples of Slaughtered Camels by Polymerase Chain Reaction (PCR) in Iran

2014 ◽  
Vol 64 (2) ◽  
pp. 245-256 ◽  
Author(s):  
Faham Khamesipour ◽  
Ebrahim Rahimi ◽  
Amir Shakerian ◽  
Abbas Doosti ◽  
Hassan Momtaz
Keyword(s):  
Lymph Node ◽  
Brucella Abortus ◽  
Brucella Melitensis ◽  
Molecular Study ◽  
Confirmatory Test ◽  
Age Group ◽  
Blood Samples ◽  
Sensitive Technique ◽  
Polymerase Chain ◽  
Control And Eradication

Abstract Brucellosis is a zoonotic disease which is characterized by reduced fertility and abortion in several species of animals, as well as humans. Camel brucellosis is caused by Brucella abortus and Brucella melitensis. To overcome the limitations posed by other techniques such as culture and serology, a sensitive technique (PCR) was employed for the detection of brucellosis in 123 camels. Findings from this PCR study indicated a total of 11.38% of blood samples as positive for Brucella spp. and 13.01% of the lymph node samples were positive for Brucella spp. In this study, 5 out of 123 (4.065%) and 3 out of 123 (2.439%) camel blood samples were positive for B. abortus and B. melitensis, respectively. Also, 4 out of 123 (3.252%) and 2 out of 123 (1.626%) camel lymph node samples were positive for B. abortus and B. melitensis, respectively. Young camels were the most commonly infected age group, while adult camels were the less often infected age group. Also, higher prevalence of brucellosis was observed in female camels. These results have indicated that PCR is a sensitive technique which could be used as a confirmatory test for the detection of brucellosis in live camels, at the same time with the lowest risk of infection of laboratory personnel. The obtained results suggest that control and eradication programs for Brucella spp. infection seem to be necessary in camels. Our findings support the power of PCR test for Brucella spp. detection in the blood and lymph node samples and it could be easily used for routine diagnosis.

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