Comparison of two selective media for the isolation of Brucella melitensis from naturally infected sheep and goats

1996 ◽  
Vol 138 (17) ◽  
pp. 409-411 ◽  
Author(s):  
C. M. Marin ◽  
M. P. Jimenez de Bagues ◽  
M. Barberan ◽  
J. M. Blasco
Keyword(s):  
Brucella Melitensis ◽  
Infected Sheep ◽  
Selective Media ◽  
Sheep And Goats

scholarly journals Immunohistochemical Detection of Brucella Melitensis Antigens in Cases of Naturally Occurring Abortions in Sheep

2008 ◽  
Vol 20 (6) ◽  
pp. 803-806 ◽  
Author(s):  
Fatma Ilhan ◽  
Zabit Yener
Keyword(s):  
Kupffer Cells ◽  
Brucella Melitensis ◽  
Immunohistochemical Detection ◽  
Zoonotic Pathogen ◽  
Sheep And Goats ◽  
Naturally Occurring ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Microscopic Studies ◽  
Formalin Fixed

Brucella melitensis, a worldwide zoonotic pathogen, is a significant cause of abortion in sheep and goats in some countries. The present study was carried out to determine, by immunohistochemistry, the presence of B. melitensis antigens in 110 naturally occurring aborted sheep fetuses. Sections of lung, liver, kidney, and spleen of each fetus were stained with immunoperoxidase to detect Brucella antigens. Brucella melitensis antigens were detected in 33 of 110 fetuses (30%). In the 33 positive cases, Brucella antigens were found in lung (25 [22.7%]), liver (21 [19%]), spleen (13 [11.8%]), and kidney (6 [5.4%]). Microscopic studies demonstrated that Brucella antigens were mainly located in the cytoplasm of macrophages and neutrophils of the lung, and in the cytoplasm of macrophages in the portal infiltrates and Kupffer cells of the liver. It was concluded that immunohistochemistry in formalin-fixed, paraffin-embedded tissues is a useful tool for the diagnosis of spontaneous ovine abortion caused by B. melitensis.

The Conglutinating Complement Absorption Test for the Sero-diagnosis of Cysticercus tenuicollis Infection in Sheep and Goats

1973 ◽  
Vol 47 (2) ◽  
pp. 191-197 ◽  
Author(s):  
T. L. Varma ◽  
S. B. Kulshrespha ◽  
B. V. Rao ◽  
S. Kumar
Keyword(s):  
Antibody Response ◽  
Infected Sheep ◽  
Absorption Test ◽  
Taenia Hydatigena ◽  
Sheep And Goats ◽  
Cysticercus Tenuicollis

The conglutinating complement absorption test (CCAT) has been used to detect Cysticercus tenuicollis (Taenia hydatigena cysticercus) infection in sheep and goats. Sera from naturally infected sheep and goats showing a titre above 1:40 were considered positive for cysticercosis. Experimentally infected lambs gave a higher antibody response than sheep and goats.

scholarly journals Cloning, Nucleotide Sequence, and Expression of theBrucella melitensis sucB Gene Coding for an Immunogenic Dihydrolipoamide Succinyltransferase Homologous Protein

2001 ◽  
Vol 69 (10) ◽  
pp. 6537-6540 ◽  
Author(s):  
Michel S. Zygmunt ◽  
Marı́a A. Dı́az ◽  
Ana P. Teixeira-Gomes ◽  
Axel Cloeckaert
Keyword(s):  
Escherichia Coli ◽  
Brucella Abortus ◽  
Brucella Melitensis ◽  
Infected Sheep ◽  
Immunogenic Protein ◽  
Gene Encoding ◽  
Homologous Protein ◽  
Gene Coding ◽  
Dihydrolipoamide Succinyltransferase ◽  
Cloned Gene

ABSTRACT The Brucella melitensis sucB gene encoding the dihydrolipoamide succinyltransferase (E2o) enzyme (previously identified as an immunogenic protein in infected sheep) was cloned and sequenced. The amino acid sequence predicted from the cloned gene revealed 88.8 and 51.2% identity to the dihydrolipoamide succinyltransferase SucB protein from Brucella abortus andEscherichia coli, respectively. Sera from naturally infected sheep showed antibody reactivity against the recombinant SucB protein.

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