A technique for blood sampling the chicken from the jugular vein

AbstractA device for the collection of discrete blood samples from large animals has been developed to allow studies of physiology to be undertaken without the confounding effect of restraint. A microprocessor controlled unit (measuring 180 × 110 × 90 mm), weighing less than 1-2 kg, is mounted on the back of the experimental subject using a simple harness. A sampling line is connected to a previously inserted jugular vein catheter. Samples of blood (approx. 5 ml) are collected at pre-determined times following a start time which can be delayed by up to 48 h to allow the subject to recover from any effects of attachment of the sampler. The results from three studies suggest that the device offers a novel way to overcome a number of difficulties which occur when conventional methods are used to collect blood in experiments.

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A non-surgical jugular catheterization technique for multiple blood sampling in cats

Laboratory Animals ◽

10.1258/002367703766453029 ◽

2003 ◽

Vol 37 (3) ◽

pp. 188-192 ◽

Cited By ~ 15

Author(s):

G. A. Albarellos ◽

R. R. Bonafine ◽

V. E. Kreil ◽

L. A. Ambros ◽

L. Montoya ◽

...

Keyword(s):

Jugular Vein ◽

Clinical Signs ◽

Blood Sampling ◽

Pharmacokinetic Studies ◽

Blood Withdrawal ◽

Needle Type ◽

Catheterization Technique ◽

Minimal Damage ◽

Multiple Blood ◽

Indwelling Time

In order to perform pharmacokinetic studies involving multiple blood sampling, repeated at variable intervals of time, a simple and reliable non-surgical jugular catheterization technique was developed. Six cats were catheterized 48 times using an indwelling through-the-needle type catheter (22G and 20.3 cm) placed into the jugular vein through an over-the-needle type (20G and 32 mm). Catheters remained in place for 1–13 days (median 3 days) without loss of patency until removal. Each jugular was catheterized a range of 2–6 times, with a total indwelling time of 4–33 days. No clinical signs of phlebitis, thrombosis or sepsis were observed either during or after the studies. This technique allows an easy, non-painful, non-stressful blood withdrawal during extended sampling periods, with minimal damage of the veins.

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Standardization of a novel blood-sampling method through the jugular vein for use in the quantified [14C] 2-deoxyglucose method

Journal of Neuroscience Methods ◽

10.1016/j.jneumeth.2005.05.018 ◽

2006 ◽

Vol 150 (2) ◽

pp. 143-149 ◽

Cited By ~ 4

Author(s):

Annelyn Torres-Reverón ◽

Susan M. Melnick ◽

Stacy I. Stephenson ◽

Diana L. Dow-Edwards

Keyword(s):

Sampling Method ◽

Jugular Vein ◽

Blood Sampling ◽

Deoxyglucose Method

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Microsurgical Skills of Establishing Permanent Jugular Vein Cannulation in Rats for Serial Blood Sampling of Orally Administered Drug

Journal of Visualized Experiments ◽

10.3791/63167 ◽

2021 ◽

Author(s):

Weijia Lu ◽

Ruimin Miao ◽

Sijun Hu ◽

Junhong Liu ◽

Fanqi Jin ◽

...

Keyword(s):

Jugular Vein ◽

Blood Sampling ◽

Serial Blood ◽

Vein Cannulation ◽

Serial Blood Sampling

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Long-term blood-sampling technique in piglets

Laboratory Animals ◽

10.1258/002367786780865539 ◽

1986 ◽

Vol 20 (3) ◽

pp. 206-209 ◽

Cited By ~ 9

Author(s):

H. Takahashi

Keyword(s):

Heart Rate ◽

Jugular Vein ◽

Sampling Technique ◽

External Jugular Vein ◽

Blood Sampling ◽

Blood Cell Count ◽

Blood Samples ◽

Polyurethane Catheter ◽

Expandable Cages

A technique to enable long-term blood sampling from piglets aged 2-3 months is described. Piglets were housed individually in expandable cages and a heparinized polyurethane catheter was inserted into the external jugular vein. A technique was used which prevented the catheter from pulling out of the vein with growth of the animals. Blood samples could be obtained for more than 1 month, and levels of cortisol, glucose, white blood cell count, haematocrit, rectal temperature and heart rate were compared for samples obtained from simulated conventional venepunctures and from the cannula using this technique. It was shown that restraint and needle pricks raised these levels considerably.

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Jugular vein catheterization for repeated blood sampling in the unrestrained conscious rat

Brain Research Protocols ◽

10.1016/s1385-299x(02)00185-x ◽

2002 ◽

Vol 10 (2) ◽

pp. 84-94 ◽

Cited By ~ 110

Author(s):

K.V Thrivikraman ◽

Rebecca L Huot ◽

Paul M Plotsky

Keyword(s):

Jugular Vein ◽

Blood Sampling ◽

Conscious Rat ◽

Vein Catheterization

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Long-term blood sampling technique in the marmoset

Laboratory Animals ◽

10.1258/002367788780864475 ◽

1988 ◽

Vol 22 (2) ◽

pp. 151-153 ◽

Cited By ~ 8

Author(s):

K. T. O'Byrne

Keyword(s):

Detailed Analysis ◽

Internal Jugular Vein ◽

Reliable Method ◽

Jugular Vein ◽

Sampling Technique ◽

Blood Sampling ◽

Blood Samples ◽

Hormone Levels

A simple and reliable method for long-term blood sampling from the restrained marmoset is described. Cannulae were chronically inserted into the internal jugular vein, exteriorized and housed in a backpack system. The cannulae remained patent for 1-7 months. This system allows for rapid collection of blood samples and hence detailed analysis of circulating hormone levels.

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