Maize AUXIN-BINDING PROTEIN 1 and AUXIN-BINDING PROTEIN 4 impact on leaf growth, elongation, and seedling responsiveness to auxin and light

Botany ◽  
2012 ◽  
Vol 90 (10) ◽  
pp. 990-1006 ◽  
Author(s):  
Dejana Jurišić-Knežev ◽  
Mária Čudejková ◽  
David Zalabák ◽  
Marta Hlobilová ◽  
Jakub Rolčík ◽  
...  
Keyword(s):  
Binding Protein ◽  
Leaf Growth ◽  
Red Light ◽  
Maize Seedlings ◽  
Exogenous Auxin ◽  
Auxin Binding ◽  
Auxin Binding Protein ◽  
Auxin Binding Proteins ◽  
The Relationship

In maize, at least five auxin-binding proteins (ABPs) have been identified, yet their functions remain unclear. The present study reports the use of maize abp1, abp4, and abp1abp4 mutants to investigate the role of ABPs during maize growth and development. Single and double abp mutant plants grown in a greenhouse differ from the wild type (WT) in their leaf declination and leaf blade growth. The effect of the dark (D), blue light (BL), red light (RL), and exogenous auxin on the development of mutant seedlings was also studied. Relative to WT, etiolated mutant seedlings were shorter and showed a reduced responsiveness to exogenous auxin. In BL or RL, the responsiveness of maize seedlings to auxin was distinctly less than in D. The reducing effect of light on seedling responsiveness to auxin is mediated at least by phytochromes. The suppression of ABP1 and (or) ABP4 led to a distinct accumulation of free indole-3-acetic acid (IAA) in etiolated and light-grown seedling organs. We concluded that ABP1 and ABP4 participate in the growth of maize seedlings, mediate seedling responses to auxin, and interact with light signaling pathway(s). We also deduce a functional interaction between ABP1 and ABP4, which is that the relationship between them is light-, organ- and response-dependent.

scholarly journals The role of auxin-binding protein 1 in the expansion of tobacco leaf cells

2002 ◽  
Vol 28 (6) ◽  
pp. 607-617 ◽  
Author(s):  
Jin-Gui Chen ◽  
Shoji Shimomura ◽  
Folke Sitbon ◽  
Göran Sandberg ◽  
Alan M Jones
Keyword(s):  
Binding Protein ◽  
Tobacco Leaf ◽  
Auxin Binding ◽  
Auxin Binding Protein

AUXIN BINDING PROTEIN 4 is involved in the Ca2+/auxin-regulated expression of ZCAX3 gene in maize (Zea mays)

Botany ◽  
2014 ◽  
Vol 92 (5) ◽  
pp. 332-339
Author(s):  
Dejana Jurišić-Knežev ◽  
Véronique Bergougnoux ◽  
David Milde ◽  
Martin Fellner
Keyword(s):  
Zea Mays ◽  
Inhibitory Effect ◽  
Auxin Signaling ◽  
Loss Of Function ◽  
Significant Drop ◽  
Exogenous Auxin ◽  
Regulated Expression ◽  
Auxin Binding ◽  
Auxin Binding Proteins

As a second messenger, calcium is involved in auxin signaling, and previous studies demonstrated that Arabidopsis CAX1 (Ca2+/H+ exchanger) is involved in the auxin transduction pathway. This study was performed to investigate the possible role of auxin-binding proteins ABP1 and ABP4 in Ca2+/auxin-regulated growth in maize (Zea mays L.). We identified and cloned two AtCAX1 homologs in maize, ZCAX2 and ZCAX3. Using maize loss-of-function abp1 and abp4 mutants, the role of ABPs in Ca2+-dependent growth and in the Ca2+/auxin-regulated expression of the CAX genes was investigated in etiolated maize seedlings. Exogenous Ca2+ enhanced mesocotyl but not coleoptile growth in WT, abp1, abp4, and abp1/abp4 mutants, but the maximum stimulation was in abp4. As well, in the abp4 mutant, maximum accumulation of Ca2+ was observed when seedlings were exposed to exogenous Ca2+. In the mesocotyl of abp4 and double mutants, the expression of ZCAX3 was significantly reduced in the absence of exogenous Ca2+, whereas exogenous Ca2+ significantly up-regulated its expression in both mutants. This effect of Ca2+ was not observed in the coleoptile. In the absence of NAA, knockout of ABP4 led to significant drop of ZCAX3 expression in the mesocotyl, and exogenous auxin significantly inhibited expression of the ZCAX3 in WT, but not in abp mutants. This effect of auxin was not observed in the coleoptile. Our results indicate that in the absence of NAA, functional ABP4 is required for ZCAX3 expression and that ABP4 mediates the inhibitory effect of NAA on ZCAX3 expression. We provided evidence for a cross talk between ABP4, exogenous auxin, Ca2+, and ZCAX3 during growth of etiolated maize mesocotyl.

scholarly journals The role of Zn2+, dimerization and N-glycosylation in the interaction of Auxin-Binding Protein 1 (ABP1) with different auxins

Glycobiology ◽  
2017 ◽  
Vol 27 (12) ◽  
pp. 1109-1119 ◽  
Author(s):  
Cibele Tesser da Costa ◽  
Conrado Pedebos ◽  
Hugo Verli ◽  
Arthur Germano Fett-Neto
Keyword(s):  
Binding Protein ◽  
Auxin Binding ◽  
Auxin Binding Protein

Retention of maize auxin-binding protein in the endoplasmic reticulum: quantifying escape and the role of auxin

Planta ◽  
1997 ◽  
Vol 202 (3) ◽  
pp. 313-323 ◽  
Author(s):  
J. Henderson ◽  
J. M. Bauly ◽  
D. A. Ashford ◽  
S. C. Oliver ◽  
C. R. Hawes ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Binding Protein ◽  
Auxin Binding ◽  
Auxin Binding Protein

scholarly journals A Soluble Auxin-Binding Protein from Hyoscyamus muticus Is a Glutathione S-Transferase

1993 ◽  
Vol 102 (1) ◽  
pp. 29-34 ◽  
Author(s):  
J. Bilang ◽  
H. Macdonald ◽  
P. J. King ◽  
A. Sturm
Keyword(s):  
Binding Protein ◽  
Hyoscyamus Muticus ◽  
Glutathione S Transferase ◽  
Auxin Binding ◽  
Auxin Binding Protein

scholarly journals In silico promoter and expression analyses of rice Auxin Binding Protein 57 (ABP57)

2021 ◽  
Vol 8 (3) ◽  
pp. 741-748
Author(s):  
Farah Afiqah Baharuddin ◽  
Zhan Xuan Khong ◽  
Zamri Zainal ◽  
Noor Liyana Sukiran
Keyword(s):  
Abiotic Stress ◽  
In Silico ◽  
Binding Protein ◽  
Transcript Level ◽  
Regulatory Elements ◽  
Biochemical Properties ◽  
Auxin Receptor ◽  
Reverse Transcription Pcr ◽  
Auxin Binding ◽  
Auxin Binding Protein

Auxin Binding Protein 57 (ABP57) is one of the molecular components involved in rice response to abiotic stress. The ABP57 gene encodes an auxin receptor which functions in activating the plasma membrane H+-ATPase. Biochemical properties of ABP57 have been characterized; however, the function of ABP57, particularly on stress and hormone responses is still limited. This study was conducted to understand the regulation of ABP57 expression under abiotic stress. Thus, in silico identification of cis-acting regulatory elements (CAREs) in the promoter region of ABP57 was performed. Several motifs and transcription factor binding site (TFBS) that are involved in abiotic stress such as ABRE, DRE, AP2/EREBP, WRKY and NAC were identified. Next, expression analysis of ABP57 under drought, salt, auxin (IAA) and abscisic acid (ABA) was conducted by reverse transcription-PCR (RT-PCR) to verify the effect of these treatments on ABP57 transcript level. ABP57 was expressed at different levels in the shoot and root under drought conditions, and its expression was increased under IAA and ABA treatments. Moreover, our results showed that ABP57 expression in the root was more responsive to drought, auxin and ABA treatments compared to its transcript in the shoot. This finding suggests that ABP57 is a drought-responsive gene and possibly regulated by IAA and ABA.

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