KINETIC STUDIES OF PLANT DECARBOXYLASES AND CARBONIC ANHYDRASE

1952 ◽  
Vol 30 (3) ◽  
pp. 306-317 ◽  
Author(s):  
N. Hansl ◽  
E. R. Waygood
Keyword(s):  
Carbon Dioxide ◽  
Carbonic Anhydrase ◽  
Kinetic Studies ◽  
Enzyme Systems ◽  
Spinach Leaf ◽  
Plant Enzyme

Spinach leaf carbonic anhydrase has been used as a tool in the Krebs-Roughton technique to determine whether several plant decarboxylase systems give rise to carbon dioxide or bicarbonate as the primary end product. The results show that in addition to the urease – urea and yeast carboxylase – pyruvic systems, the plant enzyme systems decarboxylating pyruvic, oxalacetic, glutamic, and α-ketoglutaric acids produce carbon dioxide and not bicarbonate as the primary end product.

Use and Dosage of Hydrocortisone in Endotoxic Shock

PEDIATRICS ◽  
1970 ◽  
Vol 45 (4) ◽  
pp. 720-720
Author(s):  
Harry Sonnenschein ◽  
Howard A. Joos
Keyword(s):  
Carbon Dioxide ◽  
Endotoxic Shock ◽  
Endotoxin Shock ◽  
Enzyme Systems ◽  
Shwartzman Reaction ◽  
Excellent Article

We enjoyed reading Dr. Hodes's excellent article on endotoxin shock.1 It was particularly interesting to us to note that the Shwartzman reaction could be discounted in these children, and that large doses of intravenous hydrocortisone should be administered promptly on admission to the hospital, or as soon as the diagnosis is made. Dr. Hodes presented several important reasons for using hydrocortisone in endotoxin shock, to which we should like to add another. It has been well documented2 that bacteria possess enzyme systems that can modify and degrade steroids, both partially and completely to carbon dioxide and water.

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