Bud development in Picea glauca. II. Cone differentiation and early development

1977 ◽  
Vol 55 (21) ◽  
pp. 2746-2760 ◽  
Author(s):  
John N. Owens ◽  
Marje Molder
Keyword(s):  
Picea Glauca ◽  
Shoot Elongation ◽  
Accurate Method ◽  
Optimal Time ◽  
Seed Cone ◽  
Mitotic Frequency ◽  
Mother Cells ◽  
Pollen Cone ◽  
Vegetative Bud ◽  
Made In

Pollen-cone and seed-cone buds of Picea glauca (Moench) Voss occurred as either terminal or axillary buds. All apices initiated bud scales from late April until mid-July and then differentiated into vegetative, pollen-cone, or seed-cone apices. Potentially pollen-cone apices were usually smaller, had a lower mitotic frequency, and initiated fewer bud scales than potentially vegetative or seed-cone apices. In late July a marked increase in mitotic frequency occurred in differentiating reproductive apices resulting in changes in apical size, shape, and zonation. Leaf, bract, and microsporophyll initiation began at the end of July. All microsporophylls were initiated by early October when pollen-cone buds became dormant. Sporogenous cells had differentiated but meiosis had not begun. Bract initiation began in early August and ovuliferous scale initiation began in late August. Initiation of both stopped in mid-October when seed-cone buds became dormant. Megaspore mother cells were present in dormant seed-cone buds but had not begun meiosis.There was no difference in the time of vegetative, pollen-cone, or seed-cone bud differentiation at the four locations from which collections were made in 1975 and 1976. Differentiation coincided with the end of lateral shoot elongation which was during the last half of July. These results agree with another report from one location in Ontario. Temperature sums also could be an accurate method of determining the time of cone-bud differentiation if calculations were based on the end of vegetative bud dormancy rather than on more arbitrary starting dates. The methods may be applicable to other members of the Pinaceae to determine the optimal time for cone induction treatments.

Vegetative bud development and cone differentiation in Abies amabilis

1977 ◽  
Vol 55 (8) ◽  
pp. 992-1008 ◽  
Author(s):  
John N. Owens ◽  
Marje Molder
Keyword(s):  
Shoot Elongation ◽  
Leaf Primordia ◽  
Bud Burst ◽  
Lateral Shoot ◽  
Vegetative Buds ◽  
Seed Cone ◽  
Mitotic Frequency ◽  
Mother Cells ◽  
Pollen Cone ◽  
Vegetative Bud

In the trees studied, vegetative buds began development in early April, bud burst occurred in early June and shoot elongation was completed by late July. Vegetative buds initiated bud scales from mid-April until mid-July and then initiated leaf primordia until the vegetative buds became dormant in November. All axillary buds were initiated in mid-May and their bud scales were initiated until early July. During bud-scale initiation, distal vegetative lateral apices were more conical but had a mitotic frequency similar to other lateral apices. Near the end of bud-scale initiation, vegetative apices accumulated more phenolic and ergastic compounds in future pith cells than did potential seed-cone or pollen-cone apices. Bud differentiation occurred in mid-July at the end of lateral shoot elongation. During bud differentiation the mitotic frequency of pollen-cone and seed-cone apices increased much more than that of distal vegetative apices. This resulted in a marked increase in apical size and a change in apical shape and zonation that made reproductive apices easily distinguishable from vegetative apices. Bracts began to be initiated in mid-July, and ovuliferous scales, in mid-August. Both continued to be initiated until seed-cone buds became dormant in November. A single megaspore mother cell formed in each ovule before dormancy. Microsporophylls were initiated from mid-July until early September. Microsporangia began to differentiate in September and contained microspore mother cells when pollen cones became dormant in mid-October. Meiosis did not begin before dormancy. A few potential vegetative and many potential seed-cone and potential pollen-cone apices became latent during bud-scale initiation. Some potential seed-cone apices became vegetative buds. Consequently, the number of cone buds formed was determined primarily by the proportion of apices that developed fully and the pathway along which they developed.

Bud development in mountain hemlock (Tsuga mertensiana). II. Cone-bud differentiation and predormancy development

1984 ◽  
Vol 62 (3) ◽  
pp. 484-494 ◽  
Author(s):  
John N. Owens
Keyword(s):  
Shoot Elongation ◽  
Bud Development ◽  
Seed Cone ◽  
Cone Apex ◽  
Tsuga Mertensiana ◽  
Lateral Branches ◽  
Mother Cells ◽  
Pollen Cone ◽  
Bud Initiation ◽  
Vegetative Bud

Seed cones of Tsuga mertensiana (Bong) Carr. occur terminally on distal lateral branches and form from the differentiation of a terminal, previously vegetative apex, into a seed-cone apex. Pollen cones commonly occur on lateral branches and form from the differentiation of an undetermined axillary apex about 6 weeks after axillary bud initiation. Pollen cones also occasionally occur terminally. All cone buds began differentiation in late July after bud-scale initiation was complete and at about the end of lateral shoot elongation. Seed-cone buds initiated bracts and ovuliferous scales, but not ovules, before they became dormant at the end of October. Pollen-cone buds initiated all microsporophylls by early September. Microsporangia containing microspore mother cells differentiated before pollen-cone buds became dormant in mid-October. The time of cone-bud differentiation is related to vegetative bud and shoot development. The time and method of cone-bud differentiation is discussed in relation to T. heterophylla and other conifers having similar bud development.

Bud development in Sitka spruce. II. Cone differentiation and early development

1976 ◽  
Vol 54 (8) ◽  
pp. 766-779 ◽  
Author(s):  
John N. Owens ◽  
Marje Molder
Keyword(s):  
Phenolic Compounds ◽  
Early Development ◽  
Shoot Elongation ◽  
Picea Sitchensis ◽  
Lateral Shoot ◽  
Bud Development ◽  
Seed Cone ◽  
Mitotic Frequency ◽  
Mother Cells ◽  
Pollen Cone

Pollen-cone and seed-cone buds of Picea sitchensis (Bong.) Carr. are found as either terminal or axillary buds. Pollen cones are most likely to develop from small axillary apices on vigorous distal shoots or small terminal apices on less vigorous, proximal shoots. Seed cones are most likely to develop from large, distal axillary apices on vigorous shoots or smaller terminal apices on less vigorous shoots. All apices became mitotically active late in March, passed through a 3.5-month period of bud-scale initiation, and in mid-July became differentiated as vegetative, pollen-cone, or seed-cone apices. Potentially pollen-cone apices were smaller, had a lower mitotic frequency during bud-scale initiation, and produced fewer bud scales than apices which developed into seed-cone or vegetative buds. During bud-scale initiation all apices had a few strands of cells containing phenolic compounds in the developing pith. At the time of bud differentiation, the pith of vegetative apices accumulated more phenolic compounds and non-phenolic ergastic materials, whereas the pith of reproductive apices did not. This was followed by a marked increase in mitotic frequency in reproductive apices, resulting in changes in apical size and shape. Leaf, bract, and microsporophyll initiation began about the end of July. All microsporophylls were initiated by the end of August. Sporogenous cells developed, but meiosis did not occur before the pollen cones became dormant at the end of October. Two-thirds of the bracts were initiated by the end of August. The remaining bracts were initiated more slowly until dormancy. Ovuliferous scales were initiated for 3 months beginning in September, and megaspore mother cells appeared but did not undergo meiosis before seed cones became dormant at the end of November. There was no difference in the time of vegetative, pollen-cone, and seed-cone bud differentiation, which occurred at the end of lateral shoot elongation.

Promotion of flowering in western hemlock by gibberellin A4/7 applied at different stages of bud and shoot development

1989 ◽  
Vol 19 (8) ◽  
pp. 1051-1058 ◽  
Author(s):  
John N. Owens ◽  
Anna M. Colangeli
Keyword(s):  
Effective Treatment ◽  
Shoot Elongation ◽  
Shoot Development ◽  
Western Hemlock ◽  
Bud Burst ◽  
Cone Production ◽  
Seed Cone ◽  
Pollen Cone ◽  
Vegetative Bud

Cone buds were induced on container-grown and field-grown western hemlock (Tsugaheterophylla (Raf.) Sarg.) clones during a 3-year period to study the effects of time and duration of gibberellin A4/7 treatment on cone induction, sexuality of cones, and to relate these results to bud and shoot development. The most effective treatment times preceded anatomical differentiation. The most abundant pollen cones and seed cones were produced when trees were sprayed with gibberellin A4/7 before vegetative bud burst and early shoot elongation. Two to three weekly gibberellin A4/7 applications starting at preswollen and swollen-bud stages were adequate for pollen-cone production. Pollen-cone production decreased when the applications were started at vegetative bud burst or during early shoot elongation. A minimum of three weekly applications were required for seed-cone production, and applications were equally effective when started at preswollen, swollen, and vegetative bud burst stages. Seed-cone production decreased when three weekly applications were started during early shoot elongation; however, this was overcome by increasing the number of applications.

Vegetative bud development and the time and method of cone initiation in subalpine fir (Abies lasiocarpa)

1982 ◽  
Vol 60 (11) ◽  
pp. 2249-2262 ◽  
Author(s):  
John N. Owens ◽  
Hardev Singh
Keyword(s):  
Shoot Elongation ◽  
Axillary Bud ◽  
Abies Lasiocarpa ◽  
Bud Burst ◽  
Rapid Phase ◽  
Bud Development ◽  
Vegetative Buds ◽  
Seed Cone ◽  
Pollen Cone ◽  
Vegetative Bud

Vegetative terminal and axillary bud development and the time and method of cone initiation and cone bud development are described for Abies lasiocarpa (Hook.) Nutt.Cell divisions began in vegetative buds early in April. A brief period of apical enlargement was followed by bud-scale initiation for 10 weeks. Buds were initiated in the axils of some leaf primordia about the time of vegetative bud burst, 1 month after vegetative bud dormancy ended. All buds completed bud-scale initiation by the end of June, which coincided with the end of the rapid phase of lateral shoot elongation. This was followed by a 2-week period of bud differentiation, during which time few primordia were initiated, apical size increased, and apical shape and zonation changed more in reproductive than in vegetative apices. Leaf and bract initiation began by mid-July and continued until mid-October, when vegetative and seed-cone buds became dormant. Microsporophyll initiation began earlier and was nearly completed by the end of July; pollen-cone buds became dormant in mid-September.The number of cone buds is determined by the proportion of axillary bud primordia that fully developed and the pathway along which they developed. Potential seed-cone buds may become latent but more commonly differentiate into vegetative buds of low vigor. Potential pollen-cone buds frequently become latent but have not been observed to differentiate into vegetative buds. The position of the axillary bud on the shoot and of the shoot in the tree strongly influences axillary bud development in Abies.

Bud development in Larix occidentalis. II. Cone differentiation and early development

1979 ◽  
Vol 57 (14) ◽  
pp. 1557-1572 ◽  
Author(s):  
John N. Owens ◽  
Marje Molder
Keyword(s):  
Shoot Elongation ◽  
Larix Occidentalis ◽  
Short Shoot ◽  
Bud Development ◽  
Seed Cone ◽  
Long Shoots ◽  
Short Shoots ◽  
Old Trees ◽  
Mother Cells ◽  
Pollen Cone

The time and method of cone-bud differentiation and the phenology of cone-bud development were studied in 10- to 20-year-old trees growing outside their natural range and three 50-year-old trees growing within their natural range.Both pollen-cone and seed-cone buds of western larch (Larix occidentalis Nutt.) normally differentiated on short shoots that were at least 1 year old. Pollen-cone buds were commonly on proximal nonvigorous, often pendant vegetative long shoots in lower regions of the crown, whereas seed-cone buds were usually found on distal short shoots on vigorous but less pendant vegetative long shoots in upper regions of the crown.All potential cone buds were indistinguishable from potential vegetative short shoot buds during bud-scale initiation. In early June, when vegetative short shoots had begun to initiate leaves, cone-bud apices entered a period of differentiation during which time the mitotic frequency of the apices greatly increased followed by a marked increase in apical size. During differentiation, pollen-cone apices did not initiate any basal foliar organs and a short stalk resulted at the base of the cone, whereas seed-cone apices initiated a few basal foliar primordia before bract initiation began. Microsporophyll initiation began during the last half of June and initiation occurred rapidly until the end of July. Micros porangial development occurred from August to late October when fully developed pollen-cone buds became dormant. Pollen mother cells began meiosis before dormancy and overwintered at the diffuse stage. Bract initiation began about the end of June, was rapid until mid-August, then continued more slowly until seed-cone buds became dormant in late October. Ovuliferous scales were initiated acropetally from mid-August until dormancy. Cone-bud differentiation occurred at about the end of the period of vegetative lateral long shoot elongation at all locations.

Interaction between gibberellin A4/7 and root-pruning on the reproductive and vegetative processes in Douglas-fir. IV. Effects on lateral bud development

1986 ◽  
Vol 16 (2) ◽  
pp. 211-221 ◽  
Author(s):  
J. N. Owens ◽  
J. E. Webber ◽  
S. D. Ross ◽  
R. P. Pharis
Keyword(s):  
Normal Development ◽  
Shoot Elongation ◽  
Douglas Fir ◽  
Root Pruning ◽  
Lateral Shoot ◽  
Bud Development ◽  
Lateral Bud ◽  
Mitotic Frequency ◽  
Bud Initiation ◽  
Vegetative Bud

The anatomy, mitotic frequency, size, and total insoluble carbohydrate histochemistry was studied in axillary apices from 9- and 10-year-old Douglas-fir (Pseudotsugamenziesii (Mirb.) Franco) trees after cone induction treatments of root-pruning and (or) stem injections of a gibberellin A4 and A7 (GA4/7) mixture. Axillary buds were initiated at the time of root-pruning, but root-pruning treatment had no effect on axillary bud initiation. Axillary apices from control and gibberellin-treated trees were similar and followed the normal sequence of bud-scale initiation, differentiation, and leaf initiation (described previously) and no cone buds differentiated. Early development of axillary apices from root-pruned and root-pruned, gibberellin-treated trees was normal, but development became retarded near the time of vegetative bud flush. Retarded apices were small with low mitotic frequency and developed many features characteristics of latent apices. Retardation of axillary apices continued until mid-July when normal development resumed and apices differentiated into reproductive buds or vegetative buds, or became latent. The trees in which the greatest retardation of apical development occurred during lateral shoot elongation produced the most cone buds. These results are discussed in relation to hypotheses proposed to explain how cultural and gibberellin treatments affect cone induction in the Pinaceae.

Gibberellin A4/7 enhances seed-cone production in field-grown black spruce

1988 ◽  
Vol 18 (1) ◽  
pp. 139-142 ◽  
Author(s):  
Rong H. Ho
Keyword(s):  
Vitamin E ◽  
Black Spruce ◽  
Seed Orchard ◽  
Shoot Elongation ◽  
Bud Burst ◽  
Lateral Shoot ◽  
Cone Production ◽  
Seed Cone ◽  
Vegetative Bud ◽  
Needle Primordia

Black spruce (Piceamariana (Mill.) B.S.P.) grafts growing in a seed orchard were sprayed with gibberellin A4/7, and grafts and trees in families growing in arboreta were sprayed with gibberellin A4/7 and (or) vitamin E from vegetative bud burst to the end of shoot elongation. Gibberellin A4/7 was very effective in promoting seed cones and 400 mg/L appeared optimal. Vitamin E at 1000 mg/L was not effective. Vegetative bud burst occurred in mid-May and shoot elongation ended in late June. Needle primordia were visible on the apices of newly formed buds at the end of June. Reproductive buds had fewer bud scales than vegetative buds. It appeared that potential reproductive buds terminated their bud scale initiation earlier. Gibberellin A4/7 application to promote seed-cone production should be carried out before bud-type differentiation. This coincides with the end of lateral shoot elongation.

Sexual reproduction of white spruce (Picea glauca)

1979 ◽  
Vol 57 (2) ◽  
pp. 152-169 ◽  
Author(s):  
John N. Owens ◽  
Marje Molder
Keyword(s):  
Picea Glauca ◽  
Early Embryo ◽  
Bud Dormancy ◽  
Embryo Abortion ◽  
Lower Elevation ◽  
High Incidence ◽  
Cone Development ◽  
Mother Cells ◽  
Vegetative Bud ◽  
Prophase Of Meiosis

Reproductive buds broke dormancy at the same time as vegetative buds. Pollen mother cells entered prophase of meiosis immediately after dormancy and five-celled, winged pollen was mature about 6 weeks later. Megasporogenesis occurred 3 weeks after microsporogenesis and the female gametophyte was mature in about 6 weeks. Pollination occurred over about 1 week in late May or early June and fertilization occurred about 3 weeks after pollination. One to four archegonia developed. A comparable number of 16-celled proembryos usually developed within 1 week after fertilization and cotyledons began to develop about 1 month after fertilization. Simple polyembryony occurred in most ovules but cleavage polyembryony was not observed. Embryos were fully developed in late August and seeds were mature and shed in September.The small number of archegonia often present, the high incidence of self-pollination, which may have been the cause of the high frequency of early embryo abortion, and the failure of basal and distal ovules to become pollinated were major causes of empty seed.The phenology of reproductive development varied with the site and the elevation but varied little at one site in successive years. Differences were greatest early in the growing season, but by the time of fertilization, higher elevation trees which began development much later had nearly caught up with lower elevation trees and seeds from all sites were mature and shed at about the same time.Reproductive bud dormancy like vegetative bud dormancy was broken in response to photo-period rather than temperature; however, subsequent cone development was greatly affected by temperature.

Effect of GA3 treatment timing in relation to natural day length on flowering and sex expression in Chamaecyparisnootkatensis

1989 ◽  
Vol 19 (11) ◽  
pp. 1422-1428 ◽  
Author(s):  
R. C. Bower ◽  
S. D. Ross ◽  
B. G. Dunsworth
Keyword(s):  
Treatment Time ◽  
Sex Expression ◽  
Day Length ◽  
Optimal Time ◽  
Bud Development ◽  
Treatment Timing ◽  
Seed Cone ◽  
Specific Stage ◽  
Pollen Cone ◽  
Weekly Dosage

Experiments on field-grown and potted trees investigated the effect of timing of gibberellin A3 treatment in relation to natural day length on flowering and sex expression in Chamaecyparisnootkatensis. When gibberellin A3 treatment began was more important than the number of weekly sprays (two to eight), and the optimal time of treatment was similar for promotion of seed and pollen cone buds. The optimal treatment time was independent of photoperiod or its direction of change, but instead appeared to be related to a specific stage of ontogeny during early development of the bud apex. Trees growing at progressively lower elevations attained this stage earlier in spring, suggesting that the mechanism triggering initiation of bud development involved heat sums. A higher concentration of gibberellin A3 was required to initiate seed cone buds (200 mg•L−1) than pollen cone buds (100 mg•L−1), and the average weekly dosage was more important than whether gibberellin A3 was applied weekly or biweekly. Young, potted trees within a greenhouse flowered more profusely than larger but similarly treated field-grown trees, indicating that the species is a good candidate for containerized seed orchards.

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