scholarly journals Isolation and spectral characterization of phycobiliproteins

1970 ◽  
Vol 116 (2) ◽  
pp. 161-169 ◽  
Author(s):  
F. W. J. Teale ◽  
R. E. Dale
Keyword(s):  
Energy Transfer ◽  
Ionic Strength ◽  
Aqueous Medium ◽  
Fluorescence Emission ◽  
Protein Subunit ◽  
Fluorescence Excitation ◽  
Different Types ◽  
Single Protein ◽  
Polarization Spectra

Several phycobiliproteins were prepared chromatographically pure and their absorption, fluorescence-emission, fluorescence-excitation and fluorescence-excitation polarization spectra determined. Changes in these spectra with ionic strength of the aqueous medium and chromoprotein concentration were interpreted in terms of interchromophore energy transfer and protein subunit equilibria. The complexity of the polarization spectra confirms the presence of different types of chromophore, designated sensitizing (‘s’) and fluorescing (‘f’), in a single protein.

Characterization of an array of honeys of different types and botanical origins through fluorescence emission based on LEDs

Talanta ◽  
2018 ◽  
Vol 185 ◽  
pp. 196-202 ◽  
Author(s):  
Miguel Lastra-Mejías ◽  
Albertina Torreblanca-Zanca ◽  
Regina Aroca-Santos ◽  
John C. Cancilla ◽  
Jesús.G. Izquierdo ◽  
...  
Keyword(s):  
Fluorescence Emission ◽  
Different Types

Characterization of the single protein and two nucleic acids of peach rosette mosaic virus

1980 ◽  
Vol 58 (16) ◽  
pp. 1747-1754 ◽  
Author(s):  
H. F. Dias ◽  
W. R. Allen
Keyword(s):  
Mosaic Virus ◽  
Nucleotide Composition ◽  
Temperature Transition ◽  
Protein Subunit ◽  
Spot Virus ◽  
Molecular Weights ◽  
Single Protein ◽  
Ring Spot ◽  
Equilibrium Centrifugation

Purified preparations of peach rosette mosaic virus (PRMV), were shown by rate-zonal centrifugation in sucrose and equilibrium centrifugation in CsCl to be composed of two nucleoprotein components with buoyant densities of 1.47 (middle) and 1.51 (bottom) g/cm3. The virus contains two RNA species with molecular weights of 2.5 × 106 (RNA 1) and 2.2 × I06 (RNA 2), and a single protein subunit with a molecular weight of 57 000. RNA 1 and RNA 2 reside separately in components B and M, respectively. Both RNAs are required for infection thus indicating that the virus has a divided genome. The nucleotide composition of both RNAs is similar except for cytidilic acid. The hyperchromic profile for the M component is broader than that of B and the Tm value is higher (for M Tm = 55 °C; for B Tm = 48 °C). Particle disruption and release of RNA progresses slowly over the absorbance–temperature transition. Only half of the particles were dissociated at the Tm value. Freezing dissociates most of M component into RNA 2 and protein but had no effect on the B component. Sodium chloride protected the M particles from low temperature disruption. The data support the conclusion that PRMV is a nepovirus with particular properties of the tomato ring-spot virus (TomRSV) subgroup.

Qualitative changes in the fluorescence spectra of intact pea leaves after photoinhibition

1991 ◽  
Vol 69 (5-6) ◽  
pp. 399-403 ◽  
Author(s):  
Wiesław I. Gruszecki ◽  
Konka Veeranjaneyulu ◽  
Roger M. Leblanc
Keyword(s):  
Energy Transfer ◽  
Chlorophyll Fluorescence ◽  
Fluorescence Intensity ◽  
Fluorescence Spectra ◽  
Fluorescence Emission ◽  
Heat Emission ◽  
High Light ◽  
Fluorescence Excitation ◽  
As Effects ◽  
Qualitative Changes

In high light (1400 W m−2) treated, intact pea leaves, a decrease in the ratio of fluorescence emission at 685 to 730 nm and an increase in fluorescence intensity between 500 and 600 nm were observed. Furthermore, photoacoustically monitored heat emission increased slightly, and O2 evolution decreased significantly. These findings are interpreted as effects of a photoprotective mechanism separating the carotenoid pool from the chlorophylls. This is supported by fluorescence excitation measurements and the results of a study on the reversibility of the process.Key words: chlorophyll fluorescence, carotenoid fluorescence, photoinhibition, photosynthesis, energy transfer.

Electron microscopic characterization of diamond thin films and substrates for diamond heteroepitaxial growth

1989 ◽  
Vol 47 ◽  
pp. 592-593
Author(s):  
J.B. Posthill ◽  
R.P. Burns ◽  
R.A. Rudder ◽  
Y.H. Lee ◽  
R.J. Markunas ◽  
...  
Keyword(s):  
Thin Films ◽  
Wide Band ◽  
Deposition Process ◽  
Heteroepitaxial Growth ◽  
Electron Microscopic ◽  
Wide Band Gap ◽  
Lattice Matching ◽  
Different Types ◽  
Diamond Thin Films

Because of diamond’s wide band gap, high thermal conductivity, high breakdown voltage and high radiation resistance, there is a growing interest in developing diamond-based devices for several new and demanding electronic applications. In developing this technology, there are several new challenges to be overcome. Much of our effort has been directed at developing a diamond deposition process that will permit controlled, epitaxial growth. Also, because of cost and size considerations, it is mandatory that a non-native substrate be developed for heteroepitaxial nucleation and growth of diamond thin films. To this end, we are currently investigating the use of Ni single crystals on which different types of epitaxial metals are grown by molecular beam epitaxy (MBE) for lattice matching to diamond as well as surface chemistry modification. This contribution reports briefly on our microscopic observations that are integral to these endeavors.

Characterization of Different Types of Biomass Wastes Using Thermogravimetric and ICP-MS Analyses

2020 ◽  
Vol 70 (12) ◽  
pp. 4594-4600
Keyword(s):  
Thermogravimetric Analysis ◽  
Food Industry ◽  
Wood Waste ◽  
Biomass Ash ◽  
Minor Elements ◽  
Ms Analysis ◽  
Icp Ms ◽  
Different Types

The purpose of this study was to characterize some types of biomass wastes resulted from different activities such as: agriculture, forestry and food industry using thermogravimetric and ICP-MS analyses. Also, it was optimized an ICP-MS method for the determination of As, Cd and Pb from biomass ash samples. The ICP-MS analysis revealed that the highest concentration of metals (As, Cd, Pb) was recorded in the wood waste ash sample, also the thermogravimetric analysis indicated that the highest amount of ash was obtained for the same sample (26.82%). The biomass wastes mentioned in this study are alternative recyclable materials, reusable as pellets and briquettes. Keywords: ash, biomass, ICP-MS, minor elements, TG

scholarly journals Regulation of bi-color fluorescence changes of AIE supramolecular self-assembly gels by the interaction with Al3+ and energy transfer

2021 ◽  
Author(s):  
Xinxian Ma ◽  
Jinlong Yue ◽  
Bo Qiao ◽  
LIli Zhou ◽  
Yang Gao ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Self Assembly ◽  
Fluorescence Emission ◽  
Fluorescent Materials

Supramolecular fluorescent materials have attracted considerable attention in recent years since they endow specific and unique properties to materials. Nevertheless, the utilization of photo-responsive characteristics to modulate their fluorescence emission...

Characterization of different types of broiler bedding including dehydrated grass and their influence on production

2021 ◽  
Vol 92 (1) ◽  
Author(s):  
Bruna B. Przybulinski ◽  
Rodrigo G. Garcia ◽  
Maria Fernanda de C. Burbarelli ◽  
Claudia M. Komiyama ◽  
Deivid Kelly Barbosa ◽  
...  
Keyword(s):  
Different Types

scholarly journals Mutator-Suppressible Alleles of rough sheath1 and liguleless3 in Maize Reveal Multiple Mechanisms for Suppression

Genetics ◽  
2000 ◽  
Vol 154 (1) ◽  
pp. 437-446 ◽  
Author(s):  
Lisa Girard ◽  
Michael Freeling
Keyword(s):  
Untranslated Region ◽  
Mutant Allele ◽  
Negative Regulation ◽  
Wild Type ◽  
Knox Gene ◽  
Transcript Accumulation ◽  
Mu Suppression ◽  
Different Types ◽  
Rna Blot Analysis

Abstract Insertions of Mutator transposons into maize genes can generate suppressible alleles. Mu suppression is when, in the absence of Mu activity, the phenotype of a mutant allele reverts to that of its progenitor. Here we present the characterization of five dominant Mu-suppressible alleles of the knox (knotted1-like homeobox) genes liguleless3 and rough sheath1, which exhibit neomorphic phenotypes in the leaves. RNA blot analysis suggests that Mu suppression affects only the neomorphic aspect of the allele, not the wild-type aspect. Additionally, Mu suppression appears to be exerting its effects at the level of transcription or transcript accumulation. We show that truncated transcripts are produced by three alleles, implying a mechanism for Mu suppression of 5′ untranslated region insertion alleles distinct from that which has been described previously. Additionally, it is found that Mu suppression can be caused by at least three different types of Mutator elements. Evidence presented here suggests that whether an allele is suppressible or not may depend upon the site of insertion. We cite previous work on the knox gene kn1, and discuss our results in the context of interactions between Mu-encoded products and the inherently negative regulation of neomorphic liguleless3 and rough sheath1 transcription.

scholarly journals Engineering nucleosomes for generating diverse chromatin assemblies

2021 ◽  
Author(s):  
Zenita Adhireksan ◽  
Deepti Sharma ◽  
Phoi Leng Lee ◽  
Qiuye Bao ◽  
Sivaraman Padavattan ◽  
...  
Keyword(s):  
Dynamic Properties ◽  
Dna Nanostructures ◽  
Macromolecular Assemblies ◽  
Maximum Resolution ◽  
Different Types ◽  
Chromatin Structural ◽  
Dna Fragment

Abstract Structural characterization of chromatin is challenging due to conformational and compositional heterogeneity in vivo and dynamic properties that limit achievable resolution in vitro. Although the maximum resolution for solving structures of large macromolecular assemblies by electron microscopy has recently undergone profound increases, X-ray crystallographic approaches may still offer advantages for certain systems. One such system is compact chromatin, wherein the crystalline state recapitulates the crowded molecular environment within the nucleus. Here we show that nucleosomal constructs with cohesive-ended DNA can be designed that assemble into different types of circular configurations or continuous fibers extending throughout crystals. We demonstrate the utility of the method for characterizing nucleosome compaction and linker histone binding at near-atomic resolution but also advance its application for tackling further problems in chromatin structural biology and for generating novel types of DNA nanostructures. We provide a library of cohesive-ended DNA fragment expression constructs and a strategy for engineering DNA-based nanomaterials with a seemingly vast potential variety of architectures and histone chemistries.

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