Effects of DPX-4189 (2-chloro-N-(4-methoxy-6-methyl-1,3,5-triazin-2-yl)aminocarbonyl)benzenesulfonamide) on anthocyanin synthesis, phenylalanine ammonia lyase activity, and ethylene production in soybean hypocotyls

1982 ◽  
Vol 60 (6) ◽  
pp. 741-745 ◽  
Author(s):  
Jeffrey C. Suttle ◽  
Donald R. Schreiner
Keyword(s):  
Ethylene Production ◽  
Phenylalanine Ammonia Lyase ◽  
Anthocyanin Synthesis ◽  
Anthocyanin Content ◽  
Anthocyanin Accumulation ◽  
Soybean Seedlings ◽  
Pal Activity ◽  
Lyase Activity ◽  
Endogenous Ethylene ◽  
Phenylalanine Ammonia Lyase Activity

The effects of the herbicide DPX-4189 (2-chloro-N-((4-methoxy-6-methyl-1,3,5-triazin-2-yl)aminocarbonyl)benzenesulfonamide) on anthocyanin accumulation, phenylalanine ammonia lyase (PAL) activity, and ethylene production in seedlings of soybean (Glycine max L.) were investigated. Application of 1 μg DPX-4189 per plant led to an increase in anthocyanin content in soybean hypocotyls. The increase in anthocyanin content became evident 4 days after application of the herbicide. Accompanying the increase in anthocyanin content was an eightfold increase in extractable PAL activity. An increase in endogenous ethylene evolution also accompanied the increase in anthocyanin content. Application of silver nitrate (an inhibitor of ethylene action) to herbicide-treated seedlings did not prevent the increase in anthocyanin content. Application of 2-chloroethylphosphonic acid (an ethylene-releasing compound) to soybean seedlings stimulated PAL activity but had no effect on anthocyanin content. These results indicated that ethylene did not play a role in DPX-4189 mediated anthocyanin accumulation.

Effects of Herbicides on Growth and Extractable Phenylalanine Ammonia-Lyase Activity in Light- and Dark-Grown Soybean (Glycine max) Seedlings

Weed Science ◽  
1981 ◽  
Vol 29 (4) ◽  
pp. 433-439 ◽  
Author(s):  
Robert E. Hoagland ◽  
Stephen O. Duke
Keyword(s):  
Glycine Max ◽  
Phenylalanine Ammonia Lyase ◽  
Dry Weight ◽  
Root Tip ◽  
Continuous Darkness ◽  
Soybean Seedlings ◽  
Pal Activity ◽  
Lyase Activity ◽  
Phenylalanine Ammonia Lyase Activity

Effects of 16 herbicides representing 14 herbicide classes on growth and extractable phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) were examined in light- and dark-grown soybean [Glycine max(L.) Merr. ‘Hill’] seedlings. High purity (96 to 100%) herbicides were supplied via aqueous culture at various concentrations: 0.5 mM amitrole (3-amino-s-triazole), 0.1 mM atrazine [2-chloro-4-(ethylamino)-6-(isopropylamino)-s-triazine], 0.07 mM diclofop-methyl {methyl ester of 2-[4-(2,4-dichlorophenoxy)phenoxy] propanoicacid}, 0.5 mM DSMA (disodium methanearsonate), 0.2 mM fenuron (1,1-dimethyl-3-phenylurea), 0.05 mM fluridone {1-methyl-3-phenyl-[3-(trifluoromethyl)phenyl]-4(1H)-pyridinone}, 0.5 mM MH (1,2-dihydro-3,6-pyridazinedione), 0.5 mM metribuzin [4-amino-6-tert-butyl-3-(methylthio)-as-triazin-5(4H)-one], 1.8 μM nitralin [4-(methylsulfonyl)-2,6-dinitro-N,N-dipropylaniline], 0.5 mM norflurazon [4-chloro-5-(methylamino)-2-(α,α,α-trifluoro-m-tolyl)-3(2H)-pyridazinone], 0.05 mM paraquat (1,1′-dimethyl-4,4′-bipyridinium ion), 0.15 mM perfluidone {1,1,1-trifluoro-N-[2-methyl-4-(phenylsulfonyl)phenyl] methanesulfonamide}, 0.2 mM propanil (3′,4′-dichloropropionanilide), 0.1 mM propham (isopropyl carbanilate), 0.5 mM TCA (trichloroacetic acid), and 0.05 mM 2,4-D [(2,4-dichlorophenoxy)acetic acid]. Dark-grown soybean seedlings (3-day-old) were transferred to control solutions (2 mM CaSO4) or to herbicide solutions (in 2 mM CaSO4) and grown at 25 C in continuous white light (200 μE•m-2•s-1) or continuous darkness until harvested 24 or 48 h after transfer. After 48 h, growth (fresh weight, dry weight, elongation) was inhibited by most of the chemicals. Other signs of toxicity (necrosis, secondary root stunting, and root tip swelling) were noted for some treatments. Roots were most affected, although hypocotyls were generally not changed. Hypocotyl elongation was stimulated by atrazine, fluridone, and norflurazon after 48 h light. Extractable PAL activity from soybean axes was decreased by atrazine, fenuron, metribuzin, norflurazon, propanil, propham, and 2,4-D. Amitrole and paraquat were the only herbicides that increased extractable PAL activity. Other compounds tested had no effect on the enzyme. None of the herbicides significantly affected in vitro PAL activity.

Changes in L-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with Sclerotinia sclerotiorum

2013 ◽  
Vol 8 (9) ◽  
pp. 921-929
Author(s):  
Djordje Malenčić ◽  
Jelena Cvejić ◽  
Vesna Tepavčević ◽  
Mira Bursać ◽  
Biljana Kiprovski ◽  
...  
Keyword(s):  
Glycine Max ◽  
Sclerotinia Sclerotiorum ◽  
Phenylalanine Ammonia Lyase ◽  
Soybean Seedlings ◽  
Soybean Cultivars ◽  
Pal Activity ◽  
Isoflavone Content ◽  
Lyase Activity ◽  
Glycine Max L ◽  
Phenylalanine Ammonia Lyase Activity

AbstractSoybean [Glycine max (L.) Merr.] cultivars (Meli, Alisa, Sava and 1511/99) were grown up to V1 phase (first trifoliate and one node above unifoliate) and then inoculated with Sclerotinia sclerotiorum (Lib.) de Bary under controlled conditions. Changes in L-phenylalanine ammonia-lyase (PAL) activity and isoflavone phytoalexins were recorded 12, 24, 48 and 72 h after the inoculation. Results showed an increase in PAL activity in all four examined soybean cultivars 48 h after the inoculation, being the highest in Alisa (2-fold higher). Different contents of total daidzein, genistein, glycitein and coumestrol were detected in all samples. Alisa and Sava increased their total isoflavone content (33.9% and 6.2% higher than control, respectively) as well as 1511/99, although 48 h after the inoculation its content decreased significantly. Meli exhibited the highest rate of coumestrol biosynthesis (72 h after the inoculation) and PAL activity (48 h after the inoculation). All investigated cultivars are invariably susceptible to this pathogen. Recorded changes could point to possible differences in mechanisms of tolerance among them.

Relationships Between Phenylalanine Ammonia-Lyase Activity and Physiological Responses of Soybean (Glycine max) Seedlings to Herbicides

Weed Science ◽  
1983 ◽  
Vol 31 (6) ◽  
pp. 845-852 ◽  
Author(s):  
Robert E. Hoagland ◽  
Stephen O. Duke
Keyword(s):  
Glycine Max ◽  
Soluble Protein ◽  
Phenylalanine Ammonia Lyase ◽  
Soybean Seedlings ◽  
Chemical Treatments ◽  
Pal Activity ◽  
Lyase Activity ◽  
Phenylalanine Ammonia Lyase Activity ◽  
Dichlorophenoxy Acetic Acid

The effects of 16 herbicides from 14 different chemical classes on levels of soluble protein, hydroxyphenolics, anthocyanin, and chlorophyll were determined in light- and dark-grown soybean [Glycine max(L.) Merr. ‘Hill’] seedlings. Growth-reducing concentrations of the herbicides were supplied to 3-day-old dark-grown soybean seedlings in liquid culture. Soluble protein (per axis) was reduced by fluridone {1-methyl-3-phenyl-5-[3-(trifluoromethyl)phenyl]-4(1H)-pyridinone}, paraquat (1,1′-dimethyl-4,4′-bipyridinium ion), perfluidone {1,1,1-trifluoro-N-[2-methyl-4-(phenylsulfonyl)phenyl] methanesulfonamide}, and propanil (3′,4′-dichloropropionanilide) 24 or 48 h after treatment. In light-grown plants, soluble hydroxyphenolic compound levels were decreased on a per axis basis after 48 h by all chemical treatments except by atrazine [2-chloro-4-(ethylamino)-6-(isopropylamino)-s-triazine], the methyl ester of diclofop {2-[4-(2,4-dichlorophenoxy)phenoxy] propanoate}, DSMA (disodium methanearsonate), fluridone, MH (1,2-dihydro-3,6-pyridazinedione), nitralin [4-(methylsulfonyl)-2,6-dinitro-N,N-dipropylaniline], TCA (trichloroacetic acid), and 2,4-D [(2,4-dichlorophenoxy)acetic acid]. Total chlorophyll content in hypocotyls of these seedlings was decreased by fluridone, metribuzin [4-amino-6-tert-butyl-3-(methylthio)-as-triazin-5 (4H)-one], norflurazon [4-chloro-5-(methylamino)-2-(α,α,α-trifluoro-m-tolyl)-3 (2H)-pyridazinone], paraquat, and 2,4-D, but others had no significant effect. Anthocyanin accumulation in hypocotyls of 48-h light-grown seedlings was decreased by atrazine, fenuron (1,1-dimethyl-3-phenylurea), metribuzin, norflurazon, paraquat, propanil, and propham (isopropyl carbanilate). Phenylalanine ammonia-lyase (PAL) activity (previously reported work) was positively correlated with anthocyanin levels in tissues after treatment with these 16 herbicides, but not with glyphosate [N-(phosphonomethyl)glycine]. There was, however, no correlation between extractable PAL activity and chlorophyll, soluble hydroxyphenolic compounds, or soluble protein content. These results indicated that extracted PAL activities usually reflect relative in vivo activities and that PAL activity is limiting to phenylpropanoid synthesis in vivo.

scholarly journals EFFECT OF TIME/TEMPERATURE TREATMENTS ON PHENYLALANINE AMMONIA-LYASE ACTIVITY AND DEVELOPMENT OF RUSSET SPOTTING IN ICEBERG LETTUCE

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1127c-1127
Author(s):  
M.A. Ritenour ◽  
M.E. Saltveit ◽  
M.J. Ahrens
Keyword(s):  
Phenylalanine Ammonia Lyase ◽  
Phenolic Content ◽  
Subjective Evaluation ◽  
Pal Activity ◽  
Iceberg Lettuce ◽  
Lyase Activity ◽  
Highly Correlated ◽  
Phenylalanine Ammonia Lyase Activity ◽  
Kinetics Of ◽  
Practical Implications

Russet spotting (RS) is an important postharvest disorder in Iceberg lettuce (Lactuca sativa L.). Previous studies showed that RS is induced by exposure to ∼5 ppm (ul/l) ethylene at ∼5C for 3 days and is characterized by the appearance of 1 to 2 mm diam. oval, brown sunken spots along the midrib. Increases in phenylalanine ammonia-lyase (PAL) activity and phenolic content are highly correlated with RS development. Ethylene-induced PAL activity is much less at higher (12C) or lower (0C) temperatures. In this study isolated whole leaves were exposed to a log series of ethylene concentrations from 0.1 to 10 ppm at temperatures from 0.0C to 20C for up to 8 days. Tissue was transferred among these various treatments to investigate the kinetics of PAL induction, activity and deactivation, phenolic accumulation, and RS development. A subjective evaluation was then made of RS development using a 1 to 9 scoring system in which 1 was no RS, and then PAL activity and phenolic content were measured. Preliminary results indicate that ethylene-induced PAL activity was decreased more rapidly upon transfer to temperatures above 10C than to 0.0C. Accumulation of phenolic compounds and development of RS paralleled each other, and were positively related to PAL activity. Practical implications of these results will be discussed.

Regulation of Anthocyanin Synthesis in Apple Skin. III. Involvement of Phenylalanine Ammonia-lyase

1977 ◽  
Vol 4 (1) ◽  
pp. 133 ◽  
Author(s):  
JD Faragher ◽  
DJ Chalmers
Keyword(s):  
Phenylalanine Ammonia Lyase ◽  
Anthocyanin Synthesis ◽  
Lag Phase ◽  
Anthocyanin Accumulation ◽  
Single Drop ◽  
Anthocyanin Formation ◽  
Pal Activity ◽  
Fruit Skin ◽  
Constant Rate ◽  
The Relationship

The relationship between L-phenylalanine ammonia-lyase (PAL) activity and anthocyanin synthesis in apple skin was investigated. Enzyme activity was induced by exposure to white light. After a lag phase of less than 10 h, PAL activity increased to a maximum at 30 h and then declined. Anthocyanin accumuIation, after a lag phase of 20 h, continued at a constant rate for a further 100 h. The increased rate of anthocyanin accumulation which resulted from u.v. light and cycloheximide treatment of whole fruit was preceded by comparable increases in the level of PAL activity. Wounding, which stimulated anthocyanin synthesis in skin discs, caused a far greater increase in PAL activity. In wounded tissue (skin discs) neither u.v. light or cycloheximide treatment caused any increase in anthocyanin accumulation or PAL activity. Cycloheximide inhibited anthocyanin formation in skin discs and, to a lesser extent, reduced PAL activity. When cycloheximide was applied as a single drop to a whole fruit, it inhibited anthocyanin formation but increased PAL activity in the skin to which it was applied. In a surrounding ring of skin, both anthocyanin level and PAL activity were increased. Without light, anthocyanin synthesis did not occur and there was virtually no PAL activity in whole fruit skin. However, in skin discs held in the dark, PAL activity developed to the same level as in illuminated discs, but no anthocyanin accumulated. The results indicate that, although the level of PAL activity may control the rate of anthocyanin synthesis in whole fruit, it is not the only critical enzyme regulating anthocyanin synthesis in apple skin. The mechanisms by which treatments may stimulate PAL activity and anthocyanin synthesis and the possible involvement of ethylene are discussed.

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