Major Indole Alkaloids Produced in Cell Suspension Cultures of Rhazya strict a Decaisne

1986 ◽  
Vol 41 (4) ◽  
pp. 385-390 ◽  
Author(s):  
Karl-Heinz Pawelka ◽  
Joachim Stöckigt
Keyword(s):  
Cell Suspension ◽  
Indole Alkaloids ◽  
Cell Suspension Cultures ◽  
Structural Heterogeneity ◽  
Suspension Cultures ◽  
Cell Suspensions ◽  
Minor Components ◽  
Rhazya Stricta ◽  
First Time

Eleven main alkaloids were identified from cell suspension cultures of Rhazya stricta grown in 4X-medium for 15 days. The alkaloids comprised the five groups Corynanthe, Strychnos, Eburnan, Secodine, and Aspidosperma and can be regarded as being typical Rhazya alkaloids, although the Strychnos alkaloid akuammicine has been isolated for the first time from the genus Rhazya. The most abundant compound was ( + )-1,2-dehydroaspidospermidine (15 mg/l medium) whereas all other constituents were synthesized in about 5 - 10 times lower amounts. More than 15 further alkaloids were formed as minor components which have not yet been identified. Cultivated Rhazya cells have been shown to be one of the richest alkaloid sources from apocynaceous cell suspensions. The isolated compounds, because of their structural heterogeneity, cannot be presently arranged into a scheme with coherent biosynthetic sequences.

Characterization and plant regeneration of cell suspension cultures of Lycopersicon chilense

1991 ◽  
Vol 69 (10) ◽  
pp. 2257-2260 ◽  
Author(s):  
Ann Francine Greer ◽  
Zohreh Tabaeizadeh
Keyword(s):  
Plant Regeneration ◽  
Cell Suspension ◽  
Casein Hydrolysate ◽  
Leaf Explants ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Cell Suspensions ◽  
Lag Phase ◽  
Petiole Explants ◽  
Lycopersicon Chilense

To produce calli for the establishment of a cell suspension, leaf, stem, and petiole explants of Lycopersicon chilense Dun., grown in vitro and in the soil, were cultured on media containing 15 different combinations of benzylaminopurine, kinetin, and indole acetic acid. Among the three types of tissues, leaf explants showed the best response. Cell suspension cultures of L. chilense were established from leaf callus derived from soil grown plants using Murashige and Skoog's medium supplemented with casein hydrolysate (250 mg/L), coconut water (5%), and 2,4-dichlorophenoxyacetic acid (2 mg/L). Once established, cell suspensions showed a rapid growth rate with no marked lag phase. Shooting via organogenesis occurred from callus derived from cell suspensions on medium containing 2 mg/L benzylaminopurine. Regenerated plants had the same morphology as the original plants. Key words: Lycopersicon chilense, tomato, tissue culture, cell suspensions, organogenesis, plant regeneration.

Indole Alkaloids from Cell Suspension Cultures of Stemmadenia tomentosa and Voacanga africana

1982 ◽  
Vol 37 (10) ◽  
pp. 857-860 ◽  
Author(s):  
Joachim Stöckigt ◽  
Karl-Heinz Pawelka ◽  
Ana Rother ◽  
Brigitte Deus
Keyword(s):  
Cell Culture ◽  
Cell Suspension ◽  
Growth Condition ◽  
Indole Alkaloids ◽  
Normal Growth ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Normal Growth Condition ◽  
Different Types

Abstract Cell suspension cultures of Stemmadenia tomentosa synthesized under normal growth condition the eight major indole alkaloids: (-)-tabersonine, (-)-minovincinine, (+)-conoflorine (voaphyl-line), condylocarpine, (+)-tubotaiwine (dihydrocondylocarpine), (-)-norfluorocurarine (vin-canine), (-)-vinervine, and (-)-coronaridine. These alkaloids consist of the three different types, Aspidosperma, Strychnos and Iboga. In contrast, cultures o f Voacanga africana produced mainly one alkaloid group (Aspidosperma-type) represented by (-)-tabersonine, lochnericine and (-)-minovincinine. Therefore this cell culture seems to be qualified for investigation concerning the biosynthesis of Aspidosperma alkaloids.

Ketoisophorone Transformation by Marchantia polymorpha and Nicotiana tabacum Cultured Cells

2008 ◽  
Vol 63 (5-6) ◽  
pp. 403-408 ◽  
Author(s):  
Mohamed-Elamir F. Hegazy ◽  
Toshifumi Hirata ◽  
Ahmed Abdel-Lateff ◽  
Mohamed H. Abd El-Razek ◽  
Abou El-Hamd H. Mohamed ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Cell Suspension ◽  
Cultured Cells ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Marchantia Polymorpha ◽  
Minor Components

Stereospecific olefin (C=C) and carbonyl (C=O) reduction of the readily available prochiral compound ketoisophorone (2,2,6-trimethyl-2-cyclohexene-1,4-dione) (1) by Marchantia polymorpha and Nicotiana tabacum cell suspension cultures produce the chiral products (6R)-levodione (2), (4R,5S)-4-hydroxy-3,3,5-trimethylcyclohexanone (3), and (4R,6R)-actinol (4) as well as the minor components (4R)-hydroxyisophorone (5) and (4S)-phorenol (6).

Selective Biotransformation of Monoterpenoids by Cell Suspensions of Petroselinum crispum

1989 ◽  
Vol 44 (11-12) ◽  
pp. 1066-1068 ◽  
Author(s):  
A. A. Gbolade ◽  
G. B. Lockwood
Keyword(s):  
Cell Suspension ◽  
Incubation Period ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Cell Suspensions ◽  
Petroselinum Crispum

Cell suspension cultures of Petroselinum crispum (Mill) Nyman cultivars “Paramount” and “Plain-leaved” were capable of biotransforming exogenously supplied geraniol largely into nerol and minor quantities of neral and geranial. Maximal conversions into nerol (32-36%); and neral: < 1% (cult. “Plain-leaved”), <5% (cult. “Paramount”) were usually recorded by 24 h. Over the incubation period a low proportion of the substrate, 32-40% was involved in biotransformation. Cyclic and other acyclic monoterpenoids were not biotransformed.

Voafrine A and Voafrine B, New Dimeric Indole Alkaloids from Cell Suspension Cultures ofVoacanga africanaStapf

1983 ◽  
Vol 66 (8) ◽  
pp. 2525-2533 ◽  
Author(s):  
Joachim Stöckigt ◽  
Karl-Heinz Pawelka ◽  
Takao Tanahashi ◽  
Bruno Danieli ◽  
William E. Hull
Keyword(s):  
Cell Suspension ◽  
Indole Alkaloids ◽  
Cell Suspension Cultures ◽  
Suspension Cultures
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